Function of Vibrio vulnificus MARTX toxin during early gut infection
创伤弧菌 MARTX 毒素在早期肠道感染过程中的作用
基本信息
- 批准号:8586298
- 负责人:
- 金额:$ 25.33万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-12-01 至 2016-11-30
- 项目状态:已结题
- 来源:
- 关键词:AccountingBacteriaBiochemicalCaspase-1Cell Culture TechniquesCell DeathCellsCessation of lifeConsumptionDendritic CellsDiseaseEpithelialEpithelial CellsFactor VFlesh-Eating BacteriaFoodFood SafetyFutureGenetic RecombinationGrowthHarvestHistologyHospitalizationImmune systemIn VitroIndustryInfectionIntestinesInvestigationKnock-outKnowledgeLeadLibrariesLinkLiver DysfunctionMapsModelingMonitorNecrotizing fasciitisOystersParentsPathogenesisPathway interactionsPatientsPersonsPoliciesProcessPublic PolicyResistanceRoleRouteSeafoodSepsisSepticemiaShellfishSignal PathwaySystemTestingTissuesToxinVariantVibrio vulnificusVirulenceVirulence FactorsVirulentYeastscoastal watercytotoxicityin vitro Assayin vivoin vivo imagingintestinal epitheliumkillingsmacrophagemeetingsnovelnovel therapeuticspathogenscreeningsurveillance strategytissue/cell culturewhole animal imagingyeast genetics
项目摘要
DESCRIPTION (provided by applicant): Vibrio vulnificus is a natural inhabitant of coastal waters, including the US Gulf. The bacterium causes rapid septicemia after consumption of contaminated seafood (especially raw oysters), predominantly in persons with liver dysfunction. Among food-bourne pathogens, V. vulnificus is most notable for its high rates of hospitalization and death. Indeed, V. vulnificus accounts for 1% of deaths from food-bourne illness despite causing only 0.003% of illnesses. Attempts by the FDA to protect consumers by implementation of post- harvest processing mandates met with significant resistance from the shellfish harvesting industry forcing the FDA to review its policies. Thus, the study of V. vulnificus pathogenesis has become a food safety and public policy priority. Recent studies establish that cytotoxicity of V. vulnificus is predominantly associated with a large Multifunctional-Autoprocessing RTX toxin (MARTXVv). In this study, we demonstrate that this toxin is directly linked to pathogenesis by the intragastric route of infection. We propose to study the role of the MARTXVv toxin in pathogenesis using in vivo, cell culture, and biochemical systems. The focus of the proposal will be mechanisms directly impacting early infection after consumption of contaminated food and identification and characterization of specific regions of the toxin linked to tissue damage and immune system evasion.
描述(申请人提供):创伤弧菌是沿海水域的天然居民,包括美国海湾。这种细菌在食用受污染的海鲜(特别是生牡蛎)后会导致迅速败血症,主要发生在有肝功能障碍的人身上。在食源性致病菌中,创伤弧菌以其较高的住院率和死亡率而闻名。事实上,创伤弧菌占食物伯恩病死亡的1%,尽管只有0.003%的疾病是由创伤弧菌引起。FDA试图通过实施收获后加工任务来保护消费者,但遭到了贝类捕捞行业的强烈抵制,迫使FDA审查其政策。因此,创伤弧菌致病机理的研究已成为食品安全和公共政策的重点。最近的研究表明,创伤弧菌的细胞毒性主要与一种大的多功能自动处理RTX毒素(MARTXVv)有关。在这项研究中,我们证明这种毒素通过胃内感染途径与发病直接相关。我们建议利用体内、细胞培养和生化系统来研究MARTXVv毒素在发病机制中的作用。该提案的重点将是在食用受污染的食物后直接影响早期感染的机制,以及确定和表征与组织损伤和免疫系统逃避有关的毒素的特定区域。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Karla J F Satchell其他文献
Karla J F Satchell的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Karla J F Satchell', 18)}}的其他基金
Lakeside Conference on Protein Toxins and Effectors 2021
2021 年湖畔蛋白质毒素和效应物会议
- 批准号:
10318832 - 财政年份:2022
- 资助金额:
$ 25.33万 - 项目类别:
Structural Genomics Centers for Infectious Diseases
传染病结构基因组学中心
- 批准号:
9919432 - 财政年份:2017
- 资助金额:
$ 25.33万 - 项目类别:
Structural Genomics Centers for Infectious Diseases
传染病结构基因组学中心
- 批准号:
9573719 - 财政年份:2017
- 资助金额:
$ 25.33万 - 项目类别:
Structural Genomics Centers for Infectious Diseases - SARS-CoV-2 Research Activities
传染病结构基因组学中心 - SARS-CoV-2 研究活动
- 批准号:
10439426 - 财政年份:2017
- 资助金额:
$ 25.33万 - 项目类别:
Structural Genomics Centers for Infectious Diseases
传染病结构基因组学中心
- 批准号:
9573746 - 财政年份:2017
- 资助金额:
$ 25.33万 - 项目类别:
Structural Genomics Centers for Infectious Diseases
传染病结构基因组学中心
- 批准号:
9919438 - 财政年份:2017
- 资助金额:
$ 25.33万 - 项目类别:
Structural Genomics Centers for Infectious Diseases
传染病结构基因组学中心
- 批准号:
9573699 - 财政年份:2017
- 资助金额:
$ 25.33万 - 项目类别:
Structural Genomics Centers for Infectious Diseases
传染病结构基因组学中心
- 批准号:
9573713 - 财政年份:2017
- 资助金额:
$ 25.33万 - 项目类别:
相似国自然基金
Segmented Filamentous Bacteria激活宿主免疫系统抑制其拮抗菌 Enterobacteriaceae维持菌群平衡及其机制研究
- 批准号:81971557
- 批准年份:2019
- 资助金额:65.0 万元
- 项目类别:面上项目
电缆细菌(Cable bacteria)对水体沉积物有机污染的响应与调控机制
- 批准号:51678163
- 批准年份:2016
- 资助金额:64.0 万元
- 项目类别:面上项目
相似海外基金
Biochemical and functional characterization of proteins and enzymes for heme-iron acquisition and utilisation in bacteria
细菌中血红素铁获取和利用的蛋白质和酶的生化和功能表征
- 批准号:
RGPIN-2019-05182 - 财政年份:2022
- 资助金额:
$ 25.33万 - 项目类别:
Discovery Grants Program - Individual
Biochemical and functional characterization of proteins and enzymes for heme-iron acquisition and utilisation in bacteria
细菌中血红素铁获取和利用的蛋白质和酶的生化和功能表征
- 批准号:
RGPIN-2019-05182 - 财政年份:2021
- 资助金额:
$ 25.33万 - 项目类别:
Discovery Grants Program - Individual
Biochemical and functional characterization of proteins and enzymes for heme-iron acquisition and utilisation in bacteria
细菌中血红素铁获取和利用的蛋白质和酶的生化和功能表征
- 批准号:
RGPIN-2019-05182 - 财政年份:2020
- 资助金额:
$ 25.33万 - 项目类别:
Discovery Grants Program - Individual
Biochemical and functional characterization of proteins and enzymes for heme-iron acquisition and utilisation in bacteria
细菌中血红素铁获取和利用的蛋白质和酶的生化和功能表征
- 批准号:
RGPIN-2019-05182 - 财政年份:2019
- 资助金额:
$ 25.33万 - 项目类别:
Discovery Grants Program - Individual
Physiological, biochemical and engineering study of immobilized actinomycetes, bacteria, and yeasts
固定化放线菌、细菌、酵母的生理生化及工程研究
- 批准号:
16K07678 - 财政年份:2016
- 资助金额:
$ 25.33万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Biochemical and genetic characterization of mechanisms used by soil bacteria to enhance plant growth
土壤细菌促进植物生长机制的生化和遗传特征
- 批准号:
312491-2011 - 财政年份:2015
- 资助金额:
$ 25.33万 - 项目类别:
Discovery Grants Program - Individual
Biochemical and genetic characterization of mechanisms used by soil bacteria to enhance plant growth
土壤细菌促进植物生长机制的生化和遗传特征
- 批准号:
312491-2011 - 财政年份:2014
- 资助金额:
$ 25.33万 - 项目类别:
Discovery Grants Program - Individual
Genetic and biochemical characterization of cellulases from nobel deep-sea cellulolytic bacteria
诺贝尔深海纤维素分解菌纤维素酶的遗传和生化特征
- 批准号:
26850226 - 财政年份:2014
- 资助金额:
$ 25.33万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Biochemical and genetic characterization of mechanisms used by soil bacteria to enhance plant growth
土壤细菌促进植物生长机制的生化和遗传特征
- 批准号:
312491-2011 - 财政年份:2013
- 资助金额:
$ 25.33万 - 项目类别:
Discovery Grants Program - Individual
SBIR Phase I: Engineering Bacteria for Low Cost Renewable Biochemical Production
SBIR 第一阶段:用于低成本可再生生化生产的工程细菌
- 批准号:
1248229 - 财政年份:2013
- 资助金额:
$ 25.33万 - 项目类别:
Standard Grant