Translational Profile of Perivascular Fibroblasts after Spinal Cord Injury

描述(申请人提供)：脊髓损伤(SCI)导致疤痕组织的形成，发挥多种病理生理作用，包括促进伤口愈合和抑制轴突再生。这种疤痕是由啮齿动物和人类体内细胞外基质(ECM)分子的过度沉积组成的。体外试验表明成纤维细胞是抑制性细胞外基质分子的主要来源，但在体内是否也是如此尚不清楚。虽然脑膜被认为是脊髓损伤后成纤维细胞的主要来源，但最近的证据表明，血管周围的壁龛可能是瘢痕组织的替代来源。这项提议的总体目标是确定血管周围成纤维细胞的翻译图谱。 在脊髓挫伤后的疤痕形成过程中。通过结合细胞特异性mRNA分离和下一代测序，我们的研究将为了解血管周围成纤维细胞在脊髓损伤后纤维性瘢痕形成中的作用提供蓝图。

DESCRIPTION (provided by applicant): Spinal cord injury (SCI) results in formation of scar tissue that plays multiple pathophysiological roles including promoting wound healing and inhibiting axon regeneration. This scar is comprised of excess deposition of extracellular matrix (ECM) molecules in both rodents and humans. In vitro assays have implicated fibroblasts as a major source of inhibitory ECM molecules, but whether this also true in vivo is not clear. While the meninges were thought to be the primary source of fibroblasts after SCI, recent evidence indicates that the perivascular niche could be an alternative source of the scar tissue. The overall goal of this proposal is to determine the translational profile of perivascular fibroblasts during scar formation after contusive SCI. By combining cell-specific mRNA isolation with Next Generation sequencing, our studies will provide a blue-print for understanding the role of perivascular fibroblasts in fibrotic scar formation after SCI.