Translational Profile of Perivascular Fibroblasts after Spinal Cord Injury

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DESCRIPTION (provided by applicant): Spinal cord injury (SCI) results in formation of scar tissue that plays multiple pathophysiological roles including promoting wound healing and inhibiting axon regeneration. This scar is comprised of excess deposition of extracellular matrix (ECM) molecules in both rodents and humans. In vitro assays have implicated fibroblasts as a major source of inhibitory ECM molecules, but whether this also true in vivo is not clear. While the meninges were thought to be the primary source of fibroblasts after SCI, recent evidence indicates that the perivascular niche could be an alternative source of the scar tissue. The overall goal of this proposal is to determine the translational profile of perivascular fibroblasts during scar formation after contusive SCI. By combining cell-specific mRNA isolation with Next Generation sequencing, our studies will provide a blue-print for understanding the role of perivascular fibroblasts in fibrotic scar formation after SCI.