Translational Profile of Perivascular Fibroblasts after Spinal Cord Injury

脊髓损伤后血管周围成纤维细胞的转化概况

• 批准号: 8607220
• 负责人: Jae K Lee
• 金额: $ 22.79万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-01 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8607220
• 关键词: Accounting; Address; Animals; Brain; Cells; Cicatrix; Collagen; Complex; Contusions; Deposition; Dura Mater; Extracellular Matrix; Fibroblasts; Functional disorder; Goals; Green Fluorescent Proteins; Heterogeneity; Human; Infiltration; Injury; Lead; Lesion; Maps; Meningeal; Meninges; Messenger RNA; Methods; Morphology; Mus; Patients; Pattern; Pericytes; Play; Polyribosomes; Population; Printing; Process; RNA; Recovery of Function; Ribosomal Proteins; Rodent; Role; Site; Source; Spinal Cord; Spinal cord injury; Testing; Therapeutic; Time; Tissues; Transgenic Mice; Traumatic Brain Injury; Wound Healing; axon regeneration; central nervous system injury; clinical application; disability; in vitro Assay; in vivo; insight; man; mutant mouse model; next generation sequencing; novel; promoter; public health relevance; response; transcriptome sequencing

DESCRIPTION (provided by applicant): Spinal cord injury (SCI) results in formation of scar tissue that plays multiple pathophysiological roles including promoting wound healing and inhibiting axon regeneration. This scar is comprised of excess deposition of extracellular matrix (ECM) molecules in both rodents and humans. In vitro assays have implicated fibroblasts as a major source of inhibitory ECM molecules, but whether this also true in vivo is not clear. While the meninges were thought to be the primary source of fibroblasts after SCI, recent evidence indicates that the perivascular niche could be an alternative source of the scar tissue. The overall goal of this proposal is to determine the translational profile of perivascular fibroblasts during scar formation after contusive SCI. By combining cell-specific mRNA isolation with Next Generation sequencing, our studies will provide a blue-print for understanding the role of perivascular fibroblasts in fibrotic scar formation after SCI.

描述（由申请人提供）：脊髓损伤（SCI）导致瘢痕组织的形成，其发挥多种病理生理作用，包括促进伤口愈合和抑制轴突再生。这种疤痕是由啮齿动物和人类的细胞外基质（ECM）分子过度沉积组成的。体外试验表明成纤维细胞是抑制性ECM分子的主要来源，但这在体内是否也是如此尚不清楚。虽然脑膜被认为是SCI后成纤维细胞的主要来源，但最近的证据表明血管周围的小生境可能是瘢痕组织的替代来源。本提案的总体目标是确定血管周围成纤维细胞的翻译概况 在挫伤性脊髓损伤后的疤痕形成过程中。通过将细胞特异性mRNA分离与下一代测序相结合，我们的研究将为理解血管周围成纤维细胞在SCI后纤维化瘢痕形成中的作用提供蓝图。