Staphylococcal biofilm and disease

葡萄球菌生物膜和疾病

• 批准号: 8496673
• 负责人: KENNETH W. BAYLES
• 金额: $ 182.95万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8496673
• 关键词: Acquired Immunodeficiency Syndrome; Affect; Antibiotic Resistance; Arginine; Cell Death; Cessation of life; Collaborations; Commit; Communities; Complex; Development; Developmental Process; Disease; Gene Expression Regulation; Genus staphylococcus; Growth; HIV; Human; Immune response; Immunology; Left; Medical center; Metabolism; Microbial Biofilms; Natural Immunity; Nebraska; Nosocomial Infections; Physiology; Public Health; Research; Research Personnel; Role; Staphylococcal Infections; Staphylococcus aureus; Testing; Therapeutic; Universities; bioimaging; methicillin resistant Staphylococcus aureus; novel therapeutic intervention; nuclease; pathogen

DESCRIPTION (provided by applicant): Staphylococcus aureus has historically been a leading cause of nosocomial infections in humans worldwide. In the past several decades the emergence of methicillin-resistant S. aureus (MRSA) harboring multiple antibiotic resistance determinants has left relatively few therapeutic options available. Today, these strains have made their way into the community and now pose a very serious public health threat, causing more deaths per year than HIV-AIDS. The University of Nebraska Medical Center has committed to advancing research into the staphylococci with the hiring of several new researchers, and the concerted collaboration with regional investigators, who are all focused on this important pathogen. These researchers have expertise in varied aspects of the staphylococci including: biofilm development, gene regulation, physiology, and the immunology of staphylococcal infections. The hypothesis to be tested by this team in this application is that S. aureus biofilm formation involves complex developmental processes that affect the host immune response. The application includes four projects centered around staphylococcal biofilm and disease including: regulated cell death during biofilm development (Project 1, K. Bayles); effect of arginine metabolism on biofilm formation in the staphylococci (Project 2, P. Fey); the role of nuclease in biofilm development and disease (Project 3, A. Horswill); and innate immunity to S. aureus biofilm (Project 4, T. Kielian). The cores include: Biofilm Growth and Analysis Core (Core A, J. Bose); Bioimaging Core (Core B, T. Fritz); and Administrative Core (Core C, K. Bayles). Each project involves highly collaborative and synergistic research endeavors and relies heavily on the cores. These efforts will have a dramatic impact on our understanding of biofilm formation in the staphylococci and the effect it has on the host response. Ultimately, these studies will pave the way for novel therapeutic approaches for the treatment of staphylococcal infections.

描述（由申请方提供）：金黄色葡萄球菌历来是全球人类医院感染的主要原因。在过去的几十年中，耐甲氧西林的S。携带多种抗生素抗性决定簇的金黄色葡萄球菌（MRSA）留下了相对较少的可用治疗选择。今天，这些菌株已经进入社区，现在构成了非常严重的公共卫生威胁，每年造成的死亡人数超过艾滋病毒/艾滋病。内布拉斯加大学医学中心致力于推进对葡萄球菌的研究，聘请了几名新的研究人员，并与区域研究人员进行了协调合作，他们都专注于这种重要的病原体。这些研究人员在葡萄球菌的各个方面都有专业知识，包括：生物膜发育，基因调控，生理学和葡萄球菌感染的免疫学。在这个应用程序中，这个团队要测试的假设是S。金黄色葡萄球菌生物膜的形成涉及影响宿主免疫应答的复杂发育过程。该应用程序包括四个项目围绕葡萄球菌生物膜和疾病，包括：生物膜发展过程中的细胞死亡（项目1，K。Bayles）;精氨酸代谢对葡萄球菌中生物膜形成的影响（项目2，P. Fey）;核酸酶在生物膜形成和疾病中的作用（项目3，A.马的意志）;和先天免疫S。金黄色葡萄球菌生物膜（Project 4，T. Kielian）。核心包括：生物膜生长和分析核心（核心A，J. Bose）;生物成像核心（核心B，T. Fritz）;和管理核心（核心C，K。Bayles）。每个项目都涉及高度协作和协同的研究工作，并严重依赖于核心。这些努力将对我们理解葡萄球菌中生物膜的形成及其对宿主反应的影响产生巨大影响。最终，这些研究将为治疗葡萄球菌感染的新治疗方法铺平道路。