Heat shock protein 27 (HSP27) as a marker of atherosclerosis

热休克蛋白 27 (HSP27) 作为动脉粥样硬化的标志物

• 批准号: 8609507
• 负责人: William T Gerthoffer
• 金额: $ 13.54万
• 依托单位: UNIVERSITY OF SOUTH ALABAMA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-01 至 2017-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8609507
• 关键词: Acute; Acute Coronary Event; Address; African American; Age; Aging; Anti-Inflammatory Agents; Anti-inflammatory; Antiatherogenic; Apoptotic; Arterial Fatty Streak; Atherosclerosis; Biochemical; Biochemical Markers; Biological Assay; Biological Markers; Blood; Blood Proteins; Blood Vessels; Blood specimen; Cardiac Catheterization Procedures; Cardiac Death; Cardiology; Cardiovascular Diseases; Cardiovascular system; Carotid Atherosclerotic Disease; Caucasians; Caucasoid Race; Cells; Clinical; Clinical Data; Clinical Research; Cohort Studies; Comorbidity; Complex; Coronary artery; Drops; Endothelial Cells; Event; Future; Goals; HSPB1 gene; Health; Health Services Accessibility; Health system; Heat Shock Protein 27; Hospitalization; Immunoassay; Individual; Instruction; Kinetics; Knowledge; Lead; Measures; Medical Staff; Methods; Modeling; Molecular Chaperones; Molecular Sieve Chromatography; Monitor; Myocardial Infarction; Outcome; Outpatients; Patients; Phosphorylation; Pilot Projects; Plasma; Population; Predictive Value of Tests; Pregnancy; Pregnancy-Associated Plasma Protein-A; Production; Prognostic Marker; Proteins; Recovery; Research; Risk; Risk Assessment; Role; Sampling; Smooth Muscle Myocytes; Stress Tests; Study Subject; Testing; Time; Tissues; Underserved Population; acute coronary syndrome; artery occlusion; base; cardiovascular risk factor; case control; effective therapy; high risk; medically underserved; medically underserved population; minority health; molecular size; patient population; protective effect; protein complex; volunteer

PROJECT SUMMARY (See instructions): African-Americans die from atherosclerotic cardiovascular disease at a higher rate than Caucasians. African-Americans present late and suffer from multiple co-morbidities. These factors all support the need for inexpensive, non-invasive outpatient methods to identify patients at risk for an acute coronary event. There are no established biochemical markers of acute coronary syndrome (ACS) that could be routinely employed to address the disparate risk in medically underserved populations. This issue will be addressed by a 5 yr cohort study with a nested case-control component, involving 200 subjects who are patients in the USA Health System. The patient demographic will be >40% medically underserved. Plasma levels of two candidate biomarkers of ACS, Hsp27 and pregnancy associated protein A (PAPPA), will be assayed biannually in volunteers 45-80 years old who are being monitored for cardiovascular disease by medical staff of the Division of Cardiology. Both Hsp27 and PAPP-A are biochemical components of atherosclerotic plaque that have been proposed as prognostic biomarkers of cardiovascular risk. Hsp27 in vascular smooth muscle and endothelial cells is considered an "antiatherogenic" protein because it has anti-proliferative and anti-inflammatory effects. Hsp27 is secreted into the blood in all individuals. Levels diminish during aging and appear to be further reduced as plaque burden increases. The major goal of the study is test the predictive value of Hsp27 compared to the better established PAPP-A as a biomarker of acute coronary syndrome and overall cardiovascular risk. The project has three specific aims: 1. Make repeated measures of plasma biomarker levels over a 5 yr time span that establishes the baseline variability in the biomarkers with age, 2. Make repeated measures of the biomarkers after an acute coronary event to determine the utility of the biomarkers in predicting cardiovascular risk, and 3. Conduct assays of the biochemical state of Hsp27 to test for predictive value of phosphorylation or oligomer size in assessing cardiovascular risk. Successful completion of the study would enhance the validity of plasma Hsp27 levels and Hsp27 phosphorylation as simple noninvasive outpatient tests for cardiovascular risk assessment in medically underserved populations.

项目概述（见说明）：