COBRE: UNV MED SCH: CORE B: MOLECULAR EXPRESSION & TRANGENICS

COBRE：UNV MED SCH：核心 B：分子表达

• 批准号: 7960570
• 负责人: William T Gerthoffer
• 金额: $ 21.07万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7960570
• 关键词: Adenovirus Vector; Bathing; Cells; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Consult; Electroporation; Equipment; Funding; Gene Delivery; Gene Transfer; Grant; In Situ; Incubators; Institution; Mechanics; Messenger RNA; Methods; Molecular; Muscle; Plasmids; Postdoctoral Fellow; Problem Solving; Production; Progress Reports; Proteins; Reagent; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Smooth Muscle; Smooth Muscle Myocytes; Source; Time; Tissues; Training; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Viral Packaging; Viral Vector; Virus; Water; Western Blotting; Work; design; gene cloning; gene transfer vector; interest; professor; protein expression; transgene expression; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Expression and Transgenic Core (Core B) will have three major functions: 1) To provide adenoviral gene transfer vectors and protein transduction reagents; 2) To assess mRNA and protein expression for all projects by RT-PCR and western blotting and 3) To procure transgenic mice and maintain transgenic mouse lines. Dr. William T. Gerthoffer will direct the core. He will work closely with Dr. Cherie Singer, Research Assistant Professor and two senior Research Associates to organize daily activities of the Core lab. The Director will consult with Project PIs during monthly progress reports and will solve problems that arise with production or quality of the gene transfer and protein transduction vectors. Dr. Cherie Singer, Research Assistant Professor will design all requested plasmid and virus constructs and will supervise Shanti Rawat, Research Associate who will serve as the chief technician for vector production. Mrs. Rawat will clone genes of interest, produce bacterial ceils for protein expression and propagation of plasmids and build adenovirus vectors. She will be assisted by a second full-time technician, Mariam Ba, who will assist in propagating bacterial and viral vectors and viral packaging cells. Ms Ba will also assist project PIs in assessing transgene expression in cultured smooth muscle cells and tissues by RT-PCR and western blotting. She is also trained in basic muscle mechanics and tissue electroporation methods for gene delivery in situ. Core equipment (hoods, incubator, water bath, centrifuges)will also be maintained by the Core B staff.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 分子表达和转基因核心（核心 B）将具有三个主要功能：1） 提供腺病毒基因转移载体和蛋白转导试剂； 2) 评估mRNA和蛋白质 通过 RT-PCR 和蛋白质印迹法对所有项目进行表达，3) 获得转基因小鼠并维持 转基因小鼠品系。 William T. Gerthoffer 博士将指导核心工作。他将与Cherie博士密切合作 辛格、研究助理教授和两名高级研究员组织日常活动 核心实验室。总监将在每月进度报告期间与项目 PI 协商并解决问题 因基因转移和蛋白质转导载体的生产或质量而产生。切丽·辛格博士， 研究助理教授将设计所有要求的质粒和病毒构建体，并监督 Shanti Rawat，研究员，将担任载体生产的首席技术人员。拉瓦特夫人将 克隆感兴趣的基因，产生用于蛋白质表达和质粒增殖的细菌细胞并构建 腺病毒载体。她将得到第二位全职技术员 Mariam Ba 的协助，她将协助 繁殖细菌和病毒载体以及病毒包装细胞。巴女士还将协助项目负责人 通过 RT-PCR 和蛋白质印迹评估培养的平滑肌细胞和组织中的转基因表达。她还接受过基本肌肉力学和原位基因传递组织电穿孔方法的培训。 核心设备（通风柜、培养箱、水浴锅、离心机）也将由核心 B 人员维护。