Non-Invasive Zoonotic Pathogen Exposure & Outcome Biomarkers

非侵入性人畜共患病原体暴露

• 批准号: 8699740
• 负责人: Christopher D Heaney
• 金额: $ 10.8万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8699740
• 关键词: Non; Invasive; Zoonotic; Pathogen; Exposure

DESCRIPTION (provided by applicant): This is a NIOSH Mentored Research Scientist Development Award (K01) grant proposal intended to promote the career of Dr. Christopher D. Heaney, PhD, MS, a W.K. Kellogg Health Scholar-Community Track post- doctoral fellow at the University of North Carolina, into an independent health scientist. I am a trained epidemiologist and microbiologist with a significant track record of research in community-based participatory research (CBPR) and environmental epidemiology. My goal is to integrate my existing experience with focused training in microbiology, immunology, and the molecular epidemiology of zoonotic pathogens, to become an independent scientist at the interface of basic science, occupational health practice, and population research. The topical area for the proposed career development is integration of bench science with methods for observational panel studies and CBPR. New disciplinary training will be integrated into my existing track record of CBPR with livestock workers and household members in NC to study the temporal nature of relationships between livestock exposure, zoonotic pathogen carriage, and symptoms of subclinical colonization versus infection. During this mentored award, I will expand my clinical microbiology and immunology training and epidemiologic research skills through mentorship from zoonotic infectious diseases physicians, clinical immunologists, microbiologists, and occupational health epidemiologists; engagement in formal didactics; attending multidisciplinary seminars, journal clubs, and scientific meetings; participation in study activities with my mentors; and by leadership of a project that integrates non-invasive zoonotic pathogen biomarkers into a community-based participatory panel study. These activities will be supervised by primary mentor Dr. David Weber MD, MPH, Director of the Occupational Health Services Clinic at UNC Hospitals and Professor of Epidemiology, Medicine, Pediatrics, and Infectious Diseases and co-mentor Dr. John Schmitz, PhD, Associate Director of Clinical Microbiology/Immunology and Associate Professor of Pathology and Laboratory Medicine at the UNC School of Medicine. My mentor, co-mentor, collaborators, and advisory committee members will supplement my training with their complementary expertise, and together are fully committed to helping me reach my higher disciplinary research training and career development goals, and ensuring my successful transition from mentored to independent research scientist. During year 1, I will meet weekly with mentors Drs. Weber and Schmitz to develop, optimize and validate an ELISA for detection of hepatitis E virus (HEV) antibodies (anti-HEV) in saliva. For this purpose co-mentor Dr. Schmitz is making available laboratory facilities and equipment to run ELISAs and de- identified saliva and serum for anti-HEV optimization and spiking experiments. In year 1, I will also visit collaborator Lance Price, PhD, Director of the Center for Microbiomics and Human Health at TGen, North, to continue training in multi-locus sequence typing (MLST) and whole genome SNP analysis (WGSA) of multidrug-resistant Staphylococcus aureus (MDRSA) isolated from nasal swabs of livestock workers and household members in an ongoing cross-sectional CBPR pilot study I am leading. For this purpose collaborator Price is making available his laboratory during my visit for training. During years 1 and 2, I will expand a CBPR partnership I initiated with collaborator Devon Hall of the Rural Empowerment Association for Community Help (REACH) - from a CBPR cross-sectional MDRSA prevalence study among livestock workers and household members to a community-based participatory panel study among livestock workers and household members in NC. The CBPR panel study will involve 200 livestock worker and household member participants with baseline and biweekly follow-ups for a period of 4 months. In years 2 and 3, I will meet weekly with Drs. Weber and Schmitz and collaborators to integrate the salivary anti-HEV ELISA and MDRSA genotyping methods (MLST and WGSA) into the CBPR panel study. Dr. Weber (primary mentor) and Stanley Lemon, MD (advisory committee member and Professor of Medicine and Infectious Diseases at UNC) have agreed to assist with medical referral and follow-up of CBPR panel study participants who develop symptoms of MDRSA and/or HEV infection after notification of carriage status (see plan in Protection of Human Subjects). With the support of my mentor, co-mentor, and collaborators, I am uniquely positioned to complete the proposed activities which build upon a strong CBPR partnership with IRB-approved protocols from an already-funded CBPR cross-sectional MDRSA prevalence study in NC. This information base will build a foundation to collect CBPR panel study data to characterize the temporal variation of livestock exposure, pathogen carriage, and symptoms of sub-clinical colonization versus infection - which will greatly improve current estimates of the burden of zoonotic pathogen exposure in livestock workers and household members in a region with intensive livestock production - NC. The proposed research is both novel and innovative because of its potential to advance causal inference in observational epidemiologic research, identify key risk factors of zoonotic pathogen exposure and points of intervention to reduce exposures and infection risks, and further promote the integration of CBPR with epidemiologic research15 to inform policy-making. The short-term benefits from this study will be a contribution to the under-studied area of the temporal trends and burden of two emerging zoonotic pathogens in livestock workers and household members. The long-term benefits will be translation of basic science methods for non-invasive measurement of zoonotic pathogens in nasal and saliva swabs from the bench to community to inform occupational health policies related to food animal production.

描述（由申请人提供）：这是一个NIOSH指导研究科学家发展奖（K 01）赠款提案，旨在促进克里斯托弗博士的职业生涯。希尼博士硕士W.K.凯洛格健康学者-社区跟踪博士后研究员在北卡罗来纳州大学，成为一个独立的健康科学家。我是一名训练有素的流行病学家和微生物学家，在基于社区的参与性研究（CBPR）和环境流行病学方面有着重要的研究记录。我的目标是将我现有的经验与微生物学，免疫学和人畜共患病病原体的分子流行病学的重点培训相结合，成为基础科学，职业卫生实践和人口研究界面的独立科学家。拟议职业发展的主题领域是将实验室科学与观察小组研究和CBPR方法相结合。新的学科培训将被整合到我现有的CBPR跟踪记录与畜牧业工人和家庭成员在NC研究牲畜暴露，人畜共患病病原体携带，亚临床定植与感染的症状之间的关系的时间性质。在这个指导奖，我将扩大我的临床微生物学和免疫学培训和流行病学研究技能，通过人兽共患传染病医生，临床免疫学家，微生物学家和职业健康流行病学家的指导;参与正式教学;参加多学科研讨会，期刊俱乐部和科学会议;与我的导师一起参加学习活动;以及领导一个将非侵入性人畜共患病病原体生物标志物纳入以社区为基础的参与性小组研究的项目。这些活动将由主要导师大卫韦伯医学博士，公共卫生硕士，职业健康服务诊所主任，流行病学，医学，儿科学和传染病教授和共同导师约翰施密茨博士，博士，临床微生物学/免疫学副主任和病理学和实验室医学副教授在医学院的监督。我的导师，共同导师，合作者和咨询委员会成员将用他们的互补专业知识补充我的培训，并共同致力于帮助我实现更高的学科研究培训和职业发展目标，并确保我从指导到独立研究科学家的成功过渡。在第一年，我将每周与导师Weber和Schmitz博士会面，开发，优化和验证用于检测唾液中戊型肝炎病毒（HEV）抗体（抗HEV）的ELISA。为此，共同导师Dr. Schmitz正在提供实验室设施和设备，以运行ELISA和去识别唾液和血清，用于抗HEV优化和加标实验。在第一年，我还将访问合作者Lance Price，博士，TGen的微生物与人类健康中心主任，继续培训多位点序列分型（MLST）和全基因组SNP分析（WGSA）从牲畜工人和家庭成员的鼻拭子中分离的多重耐药金黄色葡萄球菌（MDRSA）在我正在领导的一项正在进行的横断面CBPR试点研究中。为此目的，合作者普莱斯在我访问培训期间提供了他的实验室。在第1年和第2年，我将扩大CBPR伙伴关系，我发起了与合作者德文郡大厅的农村赋权协会社区帮助（REACH）-从CBPR的跨部门MDRSA流行率研究畜牧业工人和家庭成员之间的社区参与性小组研究畜牧业工人和家庭成员在NC。CBPR小组研究将涉及200名畜牧业工人和家庭成员参与者，基线和每两周一次的随访持续4个月。在第2年和第3年，我将每周与Weber和Schmitz博士以及合作者会面，将唾液抗HEV ELISA和MDRSA基因分型方法（MLST和WGSA）整合到CBPR小组研究中。Weber博士（主要导师）和Stanley Lemon医学博士（顾问委员会成员兼医学和传染病教授）已同意协助CBPR小组研究参与者的医疗转诊和随访，这些参与者在通知携带状态后出现MDRSA和/或HEV感染症状（见人类受试者保护中的计划）。在我的导师、共同导师和合作者的支持下，我处于独特的地位，能够完成拟议的活动，这些活动建立在与IRB批准的方案建立的强大的CBPR合作关系基础上，这些方案来自一项已资助的NC CBPR横断面MDRSA患病率研究。该信息库将为收集CBPR小组研究数据奠定基础，以表征牲畜暴露、病原体携带和亚临床定植与感染症状的时间变化-这将大大改善目前对集约化畜牧生产地区（NC）畜牧工人和家庭成员人畜共患病病原体暴露负担的估计。拟议的研究既新颖又创新，因为它有可能在观察性流行病学研究中推进因果推理，确定人畜共患病病原体暴露的关键风险因素和干预点，以减少暴露和感染风险，并进一步促进CBPR与流行病学研究的整合，为决策提供信息。这项研究的短期效益将有助于研究畜牧业工人和家庭成员中两种新出现的人畜共患病病原体的时间趋势和负担。长期利益将是将鼻拭子和唾液拭子中非侵入性测量人畜共患病病原体的基础科学方法从实验室转化为社区，以告知与食用动物生产相关的职业卫生政策。

项目成果

Water quality, weather and environmental factors associated with fecal indicator organism density in beach sand at two recreational marine beaches.

• DOI: 10.1016/j.scitotenv.2014.07.113
• 发表时间: 2014-11-01
• 期刊: The Science of the total environment
• 作者: Heaney CD;Exum NG;Dufour AP;Brenner KP;Haugland RA;Chern E;Schwab KJ;Love DC;Serre ML;Noble R;Wade TJ
• 通讯作者: Wade TJ

Challenges in Estimating Characteristics of Staphylococcus aureus Nasal Carriage Among Humans Enrolled in Surveillance Studies.

• DOI: 10.3389/fpubh.2018.00163
• 发表时间: 2018
• 期刊: Frontiers in public health
• 影响因子: 5.2
• 作者: Le TT;Nadimpalli M;Wu J;Heaney CD;Stewart JR
• 通讯作者: Stewart JR

Source tracking swine fecal waste in surface water proximal to swine concentrated animal feeding operations.

• DOI: 10.1016/j.scitotenv.2014.12.062
• 发表时间: 2015-04-01
• 期刊: The Science of the total environment
• 作者: Heaney CD;Myers K;Wing S;Hall D;Baron D;Stewart JR
• 通讯作者: Stewart JR