Lab-on-a-chip Flow Cytometer Using COlor-Space-Time (COST) Coding Method

使用颜色时空 (COST) 编码方法的芯片实验室流式细胞仪

基本信息

批准号：
8959759
负责人：
Lawrence S. Goldstein
金额：
$ 1.72万
依托单位：
NANOCELLECT BIOMEDICAL, INC.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-01 至 2017-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8959759
关键词：
Address Aerosols Affect Algorithms Alzheimer&apos s Disease Architecture Basic Science Biochemical Biohazardous Substance Biomedical Research Businesses Calibration Cancer Immunology Science Cell Cycle Cell Separation Cell Size Cell Survival Cell surface Cells Clinic Code Color Computer software Conflict (Psychology)Cycloparaffins Detection Devices Disease Evaluation Event Face Feedback Financial compensation Flow Cytometry Fluorescence-Activated Cell Sorting Goals Gold Health Human Immunology Incidence Individual Industry Injection of therapeutic agent Laboratories Laboratory Research Marketing Measures Methods Microfluidic Microchips Mission Molds Neurons Optics Performance Phase Ploidies Polymers Process Production Property Pump Reagent Research Research Personnel Resolution Sampling Schedule Scheme Scientist Shapes Signal Transduction Sorting - Cell Movement Source Staging Stem Cell Research Stem cells System Technology Testing Time Training base cancer stem cell cell injury cell type clinical application commercialization cost design electric impedance evaluation/testing fluorescence activated cell sorter device fluorophore improved induced pluripotent stem cell innovation instrument interest micro-total analysis system neoplastic cell particle physical separation polycarbonate prevent programs prototype public health relevance shear stress software systems system architecture tool user-friendly voltage

项目摘要

DESCRIPTION (provided by applicant): Fluorescence-activated-cell-sorting (FACS) or flow cytometry enables clinicians and researchers to quantitatively characterize the physical (cell size, shape, granularity) and biochemical (DNA content, cell cycle distribution, cell surface markers, and viability) properties of cells. With the capability of high-throughput sorting to enrih biospecimens and extract rare cell types, a state-of-the-art flow cytometer makes it possible to conduct rare-event studies such as the identification or isolation of bacterial cells, stem cells, r tumor cells. However, current flow cytometers that detect multiple colors and sort cells are expensive (~$500K), complicated, hazardous, large and bulky. For these reasons, flow cytometers are often shared amongst labs, leading to conflicts in sample handling and scheduling. With a growing market expected to reach over $3 billion by 2015, flow cytometry can address a diverse array of biomedical challenges. However, there are no high performance flow cytometers with cell sorting capabilities that are affordable for any lab. Despite the increasing demand for affordable cell sorters, the flow cytometry industry faces fundamental limits in the current technology and its evolutionary path. The current system architectures are highly inefficient in accommodating and fully utilizing the increasing number of available fluorescent colors, sensitivity, ease of use, and sorting capabilities. We propose an accessible, affordable, and high performance flow cytometry technology that will allow any scientist to perform cell analysis and sorting in their own laboratory. In this Lab-to-Market Phase II program, we propose to dramatically improve our flow cytometric cell sorting platform, the WOLF Cell Sorter, including extending the dynamic range of the fluorescent detection, implementing real time cell sorting verification, expanding chip volume manufacturing, and validating the system with early adopters such as induced pluripotent stem cell researchers excited by the unique features of our system. These technology and business goals will have a direct impact on basic research and clinical applications in human health and disease.

描述（由申请人提供）：荧光激活细胞分类（FACS）或流式细胞仪使临床医生和研究人员能够定量地表征物理（细胞大小，形状，粒度）和生化（DNA含量，细胞周期，细胞周期分布，细胞表面标记和通过稳定性）的特征。具有高通量分类对富集生物测量并提取稀有细胞类型的能力，最先进的流式细胞仪可以进行罕见的研究研究，例如鉴定或分离细菌细胞，干细胞，R肿瘤细胞。但是，当前检测多种颜色和排序单元的流式细胞仪很昂贵（约500万美元），复杂，危险，大且笨重。由于这些原因，流式细胞仪通常在实验室之间共享，从而导致样本处理和调度的冲突。随着市场预计到2015年将超过30亿美元，流式细胞仪可以解决各种各样的生物医学挑战。但是，没有具有细胞分选功能的高性能流式细胞仪，可以负担得起任何实验室。尽管对负担得起的细胞分类器的需求不断增加，但流式细胞仪行业仍面临当前技术及其进化路径的基本限制。当前的系统架构在适应和充分利用可用荧光颜色，灵敏度，易用性和分类功能的数量增加方面高效。我们提出了一种可访问，负担得起和高性能的流式细胞仪技术，该技术将使任何科学家都可以在自己的实验室中进行细胞分析和分类。 In this Lab-to-Market Phase II program, we propose to dramatically improve our flow cytometric cell sorting platform, the WOLF Cell Sorter, including extending the dynamic range of the fluorescent detection, implementing real time cell sorting verification, expanding chip volume manufacturing, and validating the system with early adopters such as induced pluripotent stem cell researchers excited by the unique features of our system.这些技术和业务目标将直接影响人类健康和疾病中的基础研究和临床应用。