Hybrid nanomaterials for dynamic, intracellular radioisotope detection

用于动态细胞内放射性同位素检测的混合纳米材料

• 批准号: 8854082
• 负责人: CRAIG A ASPINWALL
• 金额: $ 18.46万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8854082
• 关键词: Antibodies; Area; Beta Particle; Binding; Binding Proteins; Biological; Biological Assay; Caliber; Carbohydrates; Categories; Cells; Chemicals; Clinical; Collection; Deposition; Detection; Development; Diffusion; Disease; Dyes; Elements; Environment; Geometry; Glucose; Gray unit of radiation dose; Half-Life; Health; Human; Hybrids; Image; Integral Membrane Protein; Intracellular Transport; Investigation; Isotopes; Label; Lead; Life; Ligands; Liquid substance; Membrane Lipids; Modeling; Molds; Molecular; Molecular Conformation; Monitor; Nucleic Acids; Optics; Pathway interactions; Penetration; Phytic Acid; Play; Polymers; Prevalence; Proteins; Radioisotopes; Radiolabeled; Research; Research Project Grants; Role; Safety; Sampling; Scintillation Counting; Signal Transduction; Signal Transduction Pathway; Silicon Dioxide; Solubility; Solutions; Surface; System; Techniques; Technology; Thick; Time; Tracer; absorption; analytical tool; aqueous; biological systems; drug discovery; extracellular; human disease; improved; insight; insulin secretion; luminescence; molecular recognition; nanomaterials; novel; particle; radiotracer; reconstitution; research clinical testing; research study; signal processing; small molecule; temporal measurement

DESCRIPTION (provided by applicant): The capability to analyze cellular signal transduction pathways with increasing sensitivity and temporal resolution plays a key role in understanding the underlying molecular pathways involved in human diseases and disorders. Among the most challenging analytes are those present at very low concentrations with high temporal variability and small molecules lacking moieties amenable for optical and/or electrochemical detection. Radioisotopic labels play key roles in the investigation of biological systems in both the research and clinical environments, particularly for small molecule signals derived from carbohydrates. Radioisotopes facilitate highly sensitive detection with minimal perturbation of the analyte, compared to fluorescent labels, etc. Beta-particle emitters, including 32P, 35S, 14C and 3H are commonly used as biological tracers due to the prevalence of these atoms in biological molecules. The most universal radioisotopic label is 3H, due to the ubiquitous presence of H in molecular systems. 3H possesses a number of inherent advantages, including low mass differences between labeled and unlabeled compounds, a reasonable half-life for storage and low energy and short penetration depth that make 3H the safest b-emitting isotope commonly used for biological analysis. Unfortunately, the low energy and short penetration depth also complicate detection of 3H compared to other radioisotopes, minimizing the ability to analyze dynamic signaling processes in single cells or small groups of cells. We propose to develop and characterize a novel core-shell nanomaterial, termed nanoSPA, functionalized with scintillating dyes for sensitive detection of low energy radioisotopes in intracellular environments. This nanomaterial is prepared by depositing a thin silica shell onto a polymer core that is doped with radioisotope- responsive scintillants. The polymer matrix facilitates energy absorption and transfer from the radioisotope to the scintillant dye, whereas addition of the silica shell increass solubility in aqueous samples, and provides an easily modified surface. nanoSPA presents a number of advantages compared to existing technologies, including: a) enhanced compatibility with aqueous samples; b) a high-surface area to volume (SA/V) ratio for improved SPA; c) an easily modified surface for attachment of biomolecules and other chemical species; and d) applicability for intracellular radioisotope imaging. The nanoSPA platform that is proposed herein will provide a key enabling technology in a wide range of applications relevant to human health. Though we will focus our initial, proof-of-concept efforts on assays relevant to glucose-regulated insulin secretion, the range of applications for this technology is extremely broad.

描述（由申请人提供）：具有越来越高的灵敏度和时间分辨率分析细胞信号转导途径的能力，在理解涉及人类疾病和失调的潜在分子途径方面起着关键作用。最具挑战性的分析物是那些浓度极低且时间变异性高的分析物，以及缺乏可用于光学和/或电化学检测的小分子分析物。放射性同位素标记在这两种研究中对生物系统的研究起着关键作用

项目成果

Fate of fluorescent core-shell silica nanoparticles during simulated secondary wastewater treatment.

在模拟的继发废水处理过程中，荧光芯壳二氧化硅纳米颗粒的命运。

• DOI: 10.1016/j.watres.2015.03.021
• 发表时间: 2015-06-15
• 期刊: WATER RESEARCH
• 影响因子: 12.8
• 作者: Otero-Gonzalez, Lila;Field, Jim A.;Calderon, Isen A. C.;Aspinwall, Craig A.;Shadman, Farhang;Zeng, Chao;Sierra-Alvarez, Reyes
• 通讯作者: Sierra-Alvarez, Reyes