Single-molecule and ensemble imaging of GPCR-G protein complexes in live cells

活细胞中 GPCR-G 蛋白复合物的单分子和整体成像

• 批准号: 8880249
• 负责人: Jonathan A Javitch
• 金额: $ 27.21万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8880249
• 关键词: Cell Surface Receptors; Cell membrane; Cell physiology; Cell surface; Cells; Complex; Coupled; Disease; Dissociation; Drug Prescriptions; Drug Receptors; Dyes; Enzymes; Equilibrium; Event; Exhibits; Family; G-Protein-Coupled Receptors; G-substrate; GTP-Binding Proteins; Health; Heterotrimeric GTP-Binding Proteins; Image; Image Analysis; Imaging technology; Immobilization; Ion Channel; Label; Left; Life; Ligand Binding; Ligands; Macromolecular Complexes; Measurement; Measures; Mediating; Membrane Proteins; Methods; Modeling; Pathway interactions; Pharmaceutical Preparations; Physiological; Published Comment; Reaction; Receptor Activation; Resolution; Sampling; Side; Signal Transduction; System; Testing; Time; drug of abuse; experience; extracellular; geranylgeranyl pyrophosphate; indexing; overexpression; protein activation; protein complex; receptor; research study; scaffold; single molecule

DESCRIPTION (provided by applicant): G protein-coupled receptors (GPCRs) are the largest family of cell surface receptors, and are the targets of a substantial fraction of all prescribed ad abused drugs. GPCRs change cellular physiology primarily by activating intracellular heterotrimeric GTP-binding proteins (G proteins). The steps involved in G protein activation include ligand binding, receptor activation, and ultimately the formation of a "coupled" complex between a ligand, an active receptor and an inactive G protein (LR*GGDP), leading directly to G protein activation. It has long been hypothesized that inactive GPCRs and G proteins associate with each other prior to receptor activation in "precoupled" or "preassembled" RGGDP complexes. However, the existence and relevance of these complexes in cells have been difficult to document, largely because methods to study transient interactions between membrane proteins have not been available. We recently succeeded in detecting preassembled complexes between GPCRs and Gq heterotrimers in living cells, and in demonstrating their physiological significance. However, these studies left a number of important questions unanswered, in part because they relied exclusively on ensemble measurements. One critical question is the lifetime of inactive-state preassembled receptor-G protein (RGGDP) complexes. Ensemble experiments suggest that the lifetime of a preassembled RGGDP complex is long compared to the active-state, coupled complex, but neither of these lifetimes can be determined using existing methods. Long-lived preassembled RGGDP complexes would allow receptors to "self-scaffold" G proteins, i.e. maintain a high local concentration of heterotrimers ready for activation. Here we propose to study RGGDP complexes using quantitative ensemble and single-molecule imaging in living cells. Ensemble imaging will allow us to determine if preassembly serves as a self-scaffolding mechanism for several different GPCRs and G protein heterotrimers. Single-molecule imaging will allow us to directly observe the formation and dissociation of inactive-state preassembled RGGDP complexes. We will thus be able to quantitatively assess the lifetimes of the macromolecular complexes important for G protein signaling. The impact of these experiments on the immediate field will be to determine if preassembly is a significant step along the pathway to G protein activation, or alternatively if itis a rare side-reaction. With respect to macromolecular interactions in general, the impact of this project will include refinement of ensemble and single-molecule imaging technology to detect membrane protein interactions in living cells, including expression systems, dyes, labeling, immobilization, imaging and analysis strategies.

描述（由申请人提供）：G 蛋白偶联受体 (GPCR) 是最大的细胞表面受体家族，并且是大部分处方药物和滥用药物的靶标。 GPCR 主要通过激活细胞内异源三聚体 GTP 结合蛋白（G 蛋白）来改变细胞生理学。 G 蛋白激活涉及的步骤包括配体结合、受体激活，以及最终在配体、活性受体和非活性 G 蛋白 (LR*GGDP) 之间形成“偶联”复合物，直接导致 G 蛋白激活。长期以来，人们一直假设非活性 GPCR 和 G 蛋白在“预偶联”或“预组装”RGGDP 复合物中的受体激活之前相互结合。然而，这些复合物在细胞中的存在和相关性很难记录，很大程度上是因为尚无研究膜蛋白之间瞬时相互作用的方法。我们最近成功地检测了活细胞中 GPCR 和 Gq 异三聚体之间的预组装复合物，并证明了它们的生理意义。然而，这些研究留下了许多未解答的重要问题，部分原因是它们完全依赖于集合测量。一个关键问题是非活性状态预组装受体 G 蛋白 (RGGDP) 复合物的寿命。集成实验表明，预组装的 RGGDP 复合体的寿命比活性状态的耦合复合体长，但使用现有方法都无法确定这些寿命。长寿命的预组装 RGGDP 复合物将允许受体“自支架”G 蛋白，即保持异源三聚体的高局部浓度，准备激活。在这里，我们建议使用活细胞中的定量集成和单分子成像来研究 RGGDP 复合物。整体成像将使我们能够确定预组装是否可以作为几种不同 GPCR 和 G 蛋白异源三聚体的自支架机制。单分子成像将使我们能够直接观察非活性状态预组装 RGGDP 复合物的形成和解离。因此，我们将能够定量评估对 G 蛋白信号传导重要的大分子复合物的寿命。这些实验对直接领域的影响将是确定预组装是否是 G 蛋白激活途径中的一个重要步骤，或者是否是一种罕见的副反应。就一般大分子相互作用而言，该项目的影响将包括改进整体和单分子成像技术，以检测活细胞中的膜蛋白相互作用，包括表达系统、染料、标记、固定、成像和分析策略。