Core C: Imaging and Cell Structure Core

核心 C：成像和细胞结构核心

• 批准号: 8910688
• 负责人: Erik L. Snapp
• 金额: $ 8.46万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8910688
• 关键词: Antibodies; Binding; Biophotonics; Cataloging; Catalogs; Cell division; Cells; Cellular Structures; Chimeric Proteins; Consultations; Cryoelectron Microscopy; Data; Dyes; Electron Microscope; Electrons; Equipment; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Image; Imaging Techniques; Imaging technology; Individual; Institution; Label; Laboratories; Laser Scanning Confocal Microscopy; Light; Liver; Measurement; Methods; Microscopic; Microscopy; Organelles; Plasmids; Preparation; Process; Proteins; Reagent; Recommendation; Research; Research Personnel; Research Support; Sampling; Services; Techniques; Technology; Therapeutic Studies; Tissues; Training; Update; Vesicle; aerobic respiration control protein; analytical tool; design; fluorescence microscope; gene therapy; insight; instrument; instrumentation; multi-photon; research study; tool

The Imaging and Cell Structure Core (ICSC) of the Liver Center provides Liver Center Investigators with the reagents, equipment, analytic tools, and expertise to perform 'state of the art' microscopy techniques. The ICSC operates in conjunction with our institution-wide Analytical Imaging Facility (AIF), which provides access to and training on maintained top ofthe line fluorescence and electron microscopes. Advanced analytical imaging techniques, available in the Core, are not readily available in individual laboratories. The Core facilitates use of imaging techniques in liver research by supporting and assisting with use of specialized instrumentation, including laser scanning confocal microscopy, deconvolution microscopy, multi-photon microscopy, and cryo-electron microscopy. In addition, the Core provides expertise in and assistance with specialized imaging techniques such as correlative microscopy, vesicle tracking, volumetric measurements, ultrastructural sample preparation, fluorescence recovery after photobleaching (FRAP), and fluorescence resonance energy transfer (FRET). Technical support for assisted or independent use of such instrumentation is provided and Liver Center (LC) Investigators receive priority and reduced rates (10%) for use of facility instrumentation and services. In addition, assistance is available for planning experiments to utilize imaging techniques, interpret light and electron microscopic data, design fluorescent protein fusions, and select appropriate fluorescent dyes and proteins. The Core has an extensive catalog of fluorescent protein plasmids, dye-labeled antibodies, organelle markers, and other reagents available to LC Investigators. In conjunction with the Gruss Lipper Biophotonics Center, the Core provides recommendations to update equipment, anticipate emerging imaging and cell structure methods, and to remain at the leading edge of imaging technology for LC Investigators.

肝脏中心的成像和细胞结构核心（ICSC）为肝脏中心研究人员提供 试剂、设备、分析工具和专业知识，以执行“最先进的”显微镜技术。的 ICSC与我们的机构范围的分析成像设施（AIF）一起运作，该设施提供 获得并接受有关维护最佳荧光显微镜和电子显微镜的培训。先进 在堆芯中可用的分析成像技术在各个实验室中并不容易获得。的 核心通过支持和协助使用肝脏研究中的成像技术， 专业仪器，包括激光扫描共聚焦显微镜，去卷积显微镜，多光子 显微镜和冷冻电子显微镜。此外，核心小组还提供专门知识和援助， 利用专门的成像技术，如相关显微镜、囊泡跟踪、体积测量、 测量、超微结构样品制备、光漂白后的荧光恢复（FRAP），以及 荧光共振能量转移（FRET）。为协助或独立使用此类药物提供技术支持 提供仪器，肝脏中心（LC）研究者获得优先权和降低的费率（10%）， 设施仪表和服务的使用。此外，还可协助规划实验， 利用成像技术，解释光和电子显微镜数据，设计荧光蛋白融合体， 并选择合适的荧光染料和蛋白质。核心有一个广泛的目录荧光 蛋白质质粒、染料标记抗体、细胞器标记物和LC可用的其他试剂 Investigators.与Gruss Lipper生物光子学中心合作， 更新设备，预测新兴的成像和细胞结构方法，并保持领先地位 LC Investigators的最新成像技术。