Development of Digital Pneumatic Microfluidic Systems For NGS Sample Preparation

用于 NGS 样品制备的数字气动微流体系统的开发

• 批准号: 8980881
• 负责人: Seth Stern
• 金额: $ 65.98万
• 依托单位: GENAPSYS, INC.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-21 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8980881
• 关键词: Address; Air; Architecture; Biological; Biomedical Research; Characteristics; Chemicals; Clinic; Clinical; DNA; DNA Sequence; DNA Sequence Analysis; Development; Devices; Elastomers; Film; Furuncles; Future; Genomics; Glass; Health; Healthcare; High temperature of physical object; Housing; Human; Individual; Laboratories; Libraries; Link; Liquid substance; Magnetism; Medical Research; Methods; Microfluidic Microchips; Microfluidics; Molecular Biology; Output; Performance; Phase; Play; Preparation; Process; Production; Protocols documentation; Pump; Reagent; Research; Resources; Risk; Sampling; Small Business Innovation Research Grant; Structure; System; Technology; Temperature; Time; Training; Transition Temperature; Vacuum; Work; base; clinical practice; commercialization; cost; cost effective; design; digital; elastomeric; empowered; flexibility; improved; innovation; instrument; instrumentation; magnetic beads; miniaturize; next generation sequencing; pressure; prototype; public health relevance; solute; surfactant; targeted sequencing; trend; wasting

 DESCRIPTION (provided by applicant): In recent years rapid improvements in DNA sequencing technology have positively and dramatically impacted biomedical research in the US and around the world. This progress is on-going, as the capabilities of DNA sequencing instruments continue to increase, while their cost continues to decline. Moreover, DNA sequencing technology is now on the verge of directly impacting health care as it becomes an integral part of clinical practice. Sequencing of DNA, however, first requires its isolation from is normal biological context, as well as its adaptation to a form compatible with sequencing technology. These activities are referred to as sample and library preparation, respectively. Recently it has become clear that the costs and logistical burdens associated with sample and library preparation are now, and will increasingly in the future, negatively impact dissemination of DNA sequencing technology. This is because, unlike DNA sequencing itself, which is automated within increasingly efficient DNA sequencing instruments, the costs and user burdens associated with sample and library preparation have not declined significantly. The overall aim of this Phase II SBIR application is to eliminate this critical bottleneck for both research and clinical DNA sequencing applications. GenapSys has developed innovative Digital Pneumatic Microfluidic (DPM) technology to address directly the cost and user-burden deficiencies of existing sample and library preparation methods. DPM chips and instruments promise to transform expensive and logistically burdensome sample and library preparation tasks to a convenient plug-and-play format that will reduce costs in existing applications and remove barriers to deployment of new ones. Unlike previous microfluidic technologies, DPM chips will be fabricated from low-cost materials, and with low-cost mass-production methods. They will be manufactured pre-loaded with virtually all the chemicals and reagents necessary for their function, and will reduce costs further by requiring only small quantities of each of these. n addition DPM instruments will be relatively inexpensive and compact, requiring only a small space in the laboratory or clinic. The specific aims of this Phase II SBIR proposal are designed to de-risk commercialization of DPM technology by optimizing our existing methods and designs, and by providing additional proof-of-concept for certain important features. This work involves optimization of our designs and manufacturing methods for microfluidic pumps and valves, improved high-temperature performance, and demonstration of methods to package and store chemicals and reagents on-chip. The proposed work culminates in demonstration of reliable and high quality library preparation by DPM chips and prototype instruments.

 描述（由适用提供）：近年来，DNA测序技术的快速改进已经对美国和世界各地的生物医学研究产生了积极影响。随着DNA测序仪器的能力继续增加，而成本继续下降，这一进展正在进行中。此外，DNA测序技术现在正处于直接影响医疗保健的边缘，因为它已成为临床实践的组成部分。但是，DNA的测序首先需要与正常的生物学环境隔离，并且适应了与测序技术兼容的形式。这些活动分别称为样本和图书馆准备。最近，很明显，与样品和图书馆准备相关的成本和后勤伯恩根（Burnens）现在已经有所增加，并且将来会增加对DNA测序技术的传播产生负面影响。这是因为，与越来越有效的DNA测序仪器中自动化的DNA测序不同，与样品和图书馆制备相关的成本和用户伯恩斯尚未显着下降。该II期SBIR应用的总体目的是消除研究和临床DNA测序应用的关键瓶颈。 Genapsys开发了创新的数字气动微流体（DPM）技术DPM芯片和仪器有望将昂贵和逻辑上的样品和图书馆准备任务转换为方便的插件格式，以降低现有应用程序的成本，并删除新公司的部署障碍。与以前的微流体技术不同，DPM芯片将通过低成本材料和低成本的质量生产方法制造。它们将被预加载，实际上将其功能所需的所有化学药品和试剂制造，并通过仅需要少量的每一种来进一步降低成本。 n添加DPM仪器将相对便宜且紧凑，只需要在实验室或诊所中一个较小的空间。该II阶段SBIR提案的具体目的旨在通过优化我们现有的方法和设计，并为某些重要功能提供其他概念概念，以降低DPM技术的危险商业化。这项工作涉及对微流体泵和阀门的设计和制造方法的优化，改善了高温性能，并演示了包装和储存化学品和试剂片的方法。拟议的工作最终通过DPM芯片和原型工具来展示可靠和高质量的图书馆准备。