Development of retinofugal parallel pathways

项目摘要/摘要 这项研究计划的总体目标是了解平行的维甲酸 途径是在发育过程中建立的。这项建议的重点是……问题 视网膜神经节细胞(RGC)轴突如何选择正确的靶点和靶层 大脑在发育过程中。RGC轴突-靶点和轴突-层匹配都很关键 视觉回路组织的各个方面，然而，人们对它们是如何发展的知之甚少 在哺乳动物身上。我们建议研究转基因技术中这些连接的发展。 特定RGC亚型表达荧光蛋白的小鼠，以及特定RGC亚型中 基因在视黄醇逃逸途径中缺失或错误表达。这样做的具体目的是 建议是1)描述使RGC携带不同 识别大脑中合适目标的视觉信息质量，2)测试 黏附分子钙粘蛋白-6控制RGC轴突靶点的假说 匹配3)检验板层从方向特异性靶向轴突的假设 上丘选择性视网膜节细胞由黏附分子介导 钙粘附素-7。这些实验的结果应该会导致对如何 哺乳动物的视觉回路被建立，并为维持和 对损伤或疾病作出反应以补充视觉回路。

Project Summary/Abstract The overall goal of this research program is to understand how parallel retinofugal pathways are established during development. This proposal focuses on the question of how retinal ganglion cell (RGC) axons select their correct targets and target layers in the brain during development. RGC axon-target and axon-layer matching are both critical aspects of visual circuit organization and yet, very little is known about how they develop in mammals. We propose to study the development of these connections in transgenic mice where specific RGC subtypes express fluorescent proteins and in which specific genes are absent or misexpressed in the retinofugal pathway. The specific aims of this proposal are to 1) characterize the cellular events that enable RGCs carrying different qualities of visual information to recognize their appropriate targets in the brain, 2) test the hypothesis that the adhesion molecule cadherin-6 controls RGC axon-target matching 3) test the hypothesis that lamina specific targeting of axons from direction selective RGCs to the superior colliculus is mediated by the adhesion molecule cadherin-7. Results from these experiments should lead to new understanding of how mammalian visual circuits are established and inform strategies for maintaining and replenishing visual circuits in response to injury or disease.