Development of retinofugal parallel pathways

视网膜脱离平行通路的发展

• 批准号: 9003053
• 负责人: Andrew D Huberman
• 金额: $ 28.79万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-02-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9003053
• 关键词: Axon; Brain; Cadherins; Cell Adhesion Molecules; Data; Development; Disease; Embryo; Event; Family; Genes; Glaucoma; Goals; Injury; Knowledge; Lead; Left; Mammals; Mediating; Modeling; Molecular; Mus; Pathway interactions; Pattern; Process; Proteins; Research; Retinal; Retinal Ganglion Cells; Route; Specificity; Testing; Transgenic Mice; Visual; Visual Pathways; Visual system structure; abstracting; cadherin 7; cadherin-6; cohort; design; injured; knock-down; novel; postnatal; programs; research study; response; response to injury; selective expression; superior colliculus Corpora quadrigemina; visual information

Project Summary/Abstract The overall goal of this research program is to understand how parallel retinofugal pathways are established during development. This proposal focuses on the question of how retinal ganglion cell (RGC) axons select their correct targets and target layers in the brain during development. RGC axon-target and axon-layer matching are both critical aspects of visual circuit organization and yet, very little is known about how they develop in mammals. We propose to study the development of these connections in transgenic mice where specific RGC subtypes express fluorescent proteins and in which specific genes are absent or misexpressed in the retinofugal pathway. The specific aims of this proposal are to 1) characterize the cellular events that enable RGCs carrying different qualities of visual information to recognize their appropriate targets in the brain, 2) test the hypothesis that the adhesion molecule cadherin-6 controls RGC axon-target matching 3) test the hypothesis that lamina specific targeting of axons from direction selective RGCs to the superior colliculus is mediated by the adhesion molecule cadherin-7. Results from these experiments should lead to new understanding of how mammalian visual circuits are established and inform strategies for maintaining and replenishing visual circuits in response to injury or disease.

项目概要/摘要 该研究计划的总体目标是了解并行视网膜离断如何 路径是在开发过程中建立的。该提案关注的问题是 视网膜神经节细胞（RGC）轴突如何选择正确的目标和目标层 发育过程中的大脑。 RGC 轴突目标和轴突层匹配都很关键 视觉回路组织的各个方面，然而，人们对它们如何发展知之甚少 在哺乳动物中。我们建议研究转基因中这些连接的发展 特定 RGC 亚型表达荧光蛋白的小鼠，以及特定 RGC 亚型表达荧光蛋白的小鼠 视网膜脱落途径中基因缺失或错误表达。本次活动的具体目标 建议是 1）表征使 RGC 携带不同的细胞事件 视觉信息的质量，以识别大脑中适当的目标，2）测试 粘附分子 cadherin-6 控制 RGC 轴突靶点的假设 匹配3）测试以下假设：层板从方向特异性靶向轴突 上丘的选择性 RGC 由粘附分子介导 钙粘蛋白-7。这些实验的结果应该会导致人们对如何进行新的理解 哺乳动物视觉回路的建立并为维持和维持的策略提供信息 补充视觉回路以应对受伤或疾病。