A system approach to the analysis of Heterotaxy Candiate Genes

分析异序候选基因的系统方法

• 批准号: 8898862
• 负责人: Mustafa K Khokha
• 金额: $ 51.45万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2019-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8898862
• 关键词: Address; Affect; Alleles; Biological Assay; Biological Models; Biology; Candidate Disease Gene; Categories; Cause of Death; Child health care; Childhood; Cilia; Clustered Regularly Interspaced Short Palindromic Repeats; Congenital Abnormality; Development; Developmental Biology; Developmental Process; Disease; Embryonic Development; Environmental Exposure; Etiology; Europe; Failure; Future; Gene Expression; Genes; Genetic; Genetic Counseling; Genetic Predisposition to Disease; Genetic Screening; Genetic screening method; Genomics; Goals; Grant; Growth; Health; Heart; Heterogeneity; Human; Human Genetics; Immune; Individual; Infant; Infant Mortality; Lead; Left; Link; Liver; Lung; Modeling; Names; Nature; Organ; Pathology; Pathway interactions; Patients; Pattern; Play; Regulator Genes; Role; Signal Pathway; Signal Transduction; Signaling Pathway Gene; Situs Inversus; Sorting - Cell Movement; Spleen; Staging; Structural Congenital Anomalies; System; Systems Biology; Testing; Time; Transforming Growth Factor beta; Validation; Xenopus; base; congenital heart disorder; falls; fitness; gain of function; gene function; genetic analysis; genetic pedigree; improved; interest; loss of function; notch protein; novel; overexpression; prognostic

DESCRIPTION (provided by applicant): Congenital malformations are the major cause of infant mortality in the US and Europe. However, we have a poor understanding of the genetic causes of congenital malformations. In order to discover these genetic causes, we and others have employed human genomics analyses on patients. In particular we have focused on Heterotaxy, a disorder of left-right patterning. Normally, our internal organs are asymmetrically distributed along the left-right axis and failure to do so can lead to severe disease including congenital heart disease, gut malrotation, and immune deficiencies. Human genetic analysis of these patients has identified many candidate genes, but the functional relevance of these genes is unclear since strong genetic evidence (second unrelated alleles) is not available for most of them. In addition, these genes are diverse and do not fall into clear pathways~ in fact, the vast majority of these candidate genes are novel to left-right patterning. For this reason, we propose a systems approach to the analysis of these heterotaxy candidate genes. We will first prioritize these genes based on available genetic evidence and then use an unbiased approach, which will include gene expression, gain of function, and loss of function analysis to determine which of these genes play a role in left- right patterning using our high-throughput model, Xenopus. Our preliminary results indicate that many but not all of these candidate genes are important for left-right patterning. Then we will take an unbiased systems approach to placing these heterotaxy candidate genes into the left-right signaling gene regulatory network. Our preliminary results demonstrate that this systems approach identifies unexpected and interesting bridges between different pathways and identifies functions not otherwise expected of known gene and identifies specific functions of genes with no known function. In this way, we hope to improve our understanding of heterotaxy and develop a general model to approach many congenital malformations.

描述（由申请人提供）：先天性畸形是美国和欧洲婴儿死亡的主要原因。然而，我们对先天性畸形的遗传原因了解甚少。为了发现这些遗传原因，我们和其他人对患者进行了人类基因组学分析。我们特别关注了异位，一种左右模式的紊乱。正常情况下，我们的内脏沿着左右轴不对称分布，如果不这样做，可能会导致严重的疾病，包括先天性心脏病、肠道旋转不良和免疫缺陷。这些患者的人类遗传分析已经确定了许多候选基因，但这些基因的功能相关性尚不清楚，因为大多数基因没有强有力的遗传证据（第二无关等位基因）。此外，这些基因是多种多样的，并没有明确的途径~事实上，是巨大的