Developing transposon methods for insertional mutagenesis in Xenopus

开发非洲爪蟾插入突变的转座子方法

• 批准号: 7895047
• 负责人: Mustafa K Khokha
• 金额: $ 20.69万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-16 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7895047
• 关键词: Biological; Biological Models; Biology; Data; Development; Developmental Biology; Embryonic Pattern Specification; Genes; Genetic; Genetic Screening; Genome; Genotype; Goals; Human Genome; Insertional Mutagenesis; Laboratories; Methods; Modeling; Mus; Mutagenesis; Mutagens; Mutate; Pattern Formation; Phenotype; Process; Rana; Research Personnel; Series; Site; System; Xenopus; analytical tool; gene function; genetic analysis; human disease; interest; new technology; novel; public health relevance; research study; tool; tool development; xenopus genome

DESCRIPTION (provided by applicant): The main goal of this proposal is to develop transposon tools in order to mutagenize the genome of Xenopus tropicalis. Recently, we have developed genetic analysis in Xenopus which is an exciting new avenue for studying gene function in this model system that is uniquely manipulable as an experimental system. A critical next step is to develop efficient mutagenesis methods. We outline a series of experiments to develop tol2 and piggybac transposons as insertional mutagens in X. tropicalis. These experiments will demonstrate that insertional mutagenesis with transposons can be an effective way to move quickly from interesting phenotype to genotype facilitating in-depth characterization of gene function. Our major interest is in early embryonic pattern formation; however, the tools that we develop in this proposal will have a wide application in diverse fields of biology. PUBLIC HEALTH RELEVANCE: The function of most genes in our genome remains a mystery. In order to determine their function, we must use model systems such as frogs specifically Xenopus. In this proposal, we will create new technologies to determine gene function in Xenopus and therefore human disease.

描述（由申请人提供）：本提案的主要目标是开发转座子工具，以诱变热带爪蟾的基因组。最近，我们在爪蟾中开发了遗传分析，这是研究该模型系统中基因功能的令人兴奋的新途径，该模型系统作为实验系统具有独特的可操作性。下一步关键是开发有效的诱变方法。我们概述了一系列实验，以开发 tol2 和 Piggybac 转座子作为热带 X. 的插入诱变剂。这些实验将证明，使用转座子的插入诱变可以是快速从有趣的表型转变为基因型的有效方法，从而促进基因功能的深入表征。我们的主要兴趣是早期胚胎模式的形成；然而，我们在本提案中开发的工具将在生物学的各个领域得到广泛应用。公共卫生相关性：我们基因组中大多数基因的功能仍然是个谜。为了确定它们的功能，我们必须使用青蛙（特别是爪蟾）等模型系统。在这项提案中，我们将创造新技术来确定非洲爪蟾的基因功能，从而确定人类疾病。

项目成果

Breeding based remobilization of Tol2 transposon in Xenopus tropicalis.

• DOI: 10.1371/journal.pone.0076807
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Lane MA;Kimber M;Khokha MK
• 通讯作者: Khokha MK