Flow Cytometry Core

流式细胞术核心

• 批准号: 8871652
• 负责人: Steven A Porcelli
• 金额: $ 2.34万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8871652
• 关键词: Address; Animals; Area; Cancer Center; Cell Culture Techniques; Cells; Containment; Core Facility; Data Analyses; Development; Ensure; Environment; Equipment; Flow Cytometry; Funding; Genus Mycobacterium; Goals; Grant; Harvest; Housing; Human Resources; Immune response; Individual; Infectious Agent; Institution; Instruction; Laboratories; Maintenance; Methods; Molecular Analysis; Mus; Mycobacterium tuberculosis; Organism; Pathogenesis; Performance; Pharmaceutical Preparations; Phenotype; Play; Population; Procedures; Program Research Project Grants; Progress Reports; Request for Proposals; Research; Research Infrastructure; Research Personnel; Resources; Role; Safety; Sampling; Schedule; Sorting - Cell Movement; Speed; Techniques; Technology; Time; Tuberculosis; Update; Vaccines; Work; cost; cost effective; design and construction; innovation; instrument; instrumentation; interest; member; mutant; mycobacterial; operation; prevent; programs; research study; success; tuberculosis immunity

Flow cytometry is one of the most widely used and indispensable techniques for analysis of cell phenotypes and for isolation of specific cell populations. The Flow Cytometry Core resources supported by the P01 provide the Program's investigators with increased access in a cost effective way to instruments for analytical flow cytometry, which in some cases must be maintained in appropriate biosafety containment conditions. The core has three specific aims: 1) Provide and maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards; 2) Provide technical assistance in the performance and interpretation of established techniques involving flow cytometry; 3) Introduce new and update existing technologies to maintain cutting edge capabilities. The core facility supported by the P01 has full responsibility for maintaining one analytical flow cytometer, and contributes to the maintenance and operation of one high speed cell sorter. Access through this core to FACS analysis and sorting techniques under Biosafety Level (BSL)-2 and BSL-3 conditions has played an essential role in research funded by this P01 grant during the past 5 years, and will continue to do so into the next funding period. The high cost of the instrumentation and the need for skilled personnel makes the establishment of a core facility for flow cytometry the only practical approach to management of these resources. In addition, this core facility provides the only possibility for the P01 investigators to do flow cytometry in a BSL-3 environment within the institution, and thus is a crucial facility for some of the studies that are ongoing or planned within the context of the program. The core also provides an environment in which biosafety containment procedures can be strictly overseen and enforced, thus ensuring the safety of personnel using flow cytometry techniques in their work with potentially dangerous infectious agents. The analytical capabilities of the core allow the program investigators to identify and characterize cell populations of interest in a wide range of studies related to mycobacterial pathogenesis and the host immune response.

流式细胞术是分析细胞表型和分离特定细胞群的最广泛使用和不可或缺的技术之一。 P01 支持的流式细胞术核心资源为该计划的研究人员提供了以经济高效的方式更多地使用分析流式细胞术仪器的机会，在某些情况下，这些仪器必须保持在适当的生物安全控制条件下。该核心有三个具体目标：1）提供和维护流式细胞术研究所需的必要研究基础设施，并采取适当的生物安全遏制保障措施； 2) 为涉及流式细胞术的既定技术的执行和解释提供技术援助； 3) 引入新技术和更新现有技术，以保持尖端能力。 P01支持的核心设施全面负责维护一台分析流式细胞仪，并有助于维护和操作一台高速细胞分选仪。在过去 5 年中，通过该核心获得生物安全级别 (BSL)-2 和 BSL-3 条件下的 FACS 分析和分类技术在 P01 资助的研究中发挥了重要作用，并将在下一个资助期间继续发挥作用。仪器的高成本和对熟练人员的需求使得建立流式细胞术核心设施成为管理这些资源的唯一实用方法。此外，该核心设施为 P01 研究人员提供了在机构内 BSL-3 环境中进行流式细胞术的唯一可能性，因此对于该计划范围内正在进行或计划进行的一些研究来说是至关重要的设施。该核心还提供了一个可以严格监督和执行生物安全遏制程序的环境，从而确保使用流式细胞术技术处理潜在危险传染源的人员的安全。核心的分析能力使项目研究人员能够在与分枝杆菌发病机制和宿主免疫反应相关的广泛研究中识别和表征感兴趣的细胞群。