Flow Cytometry Core

流式细胞术核心

• 批准号: 8871652
• 负责人: Steven A Porcelli
• 金额: $ 2.34万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8871652
• 关键词: Address; Animals; Area; Cancer Center; Cell Culture Techniques; Cells; Containment; Core Facility; Data Analyses; Development; Ensure; Environment; Equipment; Flow Cytometry; Funding; Genus Mycobacterium; Goals; Grant; Harvest; Housing; Human Resources; Immune response; Individual; Infectious Agent; Institution; Instruction; Laboratories; Maintenance; Methods; Molecular Analysis; Mus; Mycobacterium tuberculosis; Organism; Pathogenesis; Performance; Pharmaceutical Preparations; Phenotype; Play; Population; Procedures; Program Research Project Grants; Progress Reports; Request for Proposals; Research; Research Infrastructure; Research Personnel; Resources; Role; Safety; Sampling; Schedule; Sorting - Cell Movement; Speed; Techniques; Technology; Time; Tuberculosis; Update; Vaccines; Work; cost; cost effective; design and construction; innovation; instrument; instrumentation; interest; member; mutant; mycobacterial; operation; prevent; programs; research study; success; tuberculosis immunity

Flow cytometry is one of the most widely used and indispensable techniques for analysis of cell phenotypes and for isolation of specific cell populations. The Flow Cytometry Core resources supported by the P01 provide the Program's investigators with increased access in a cost effective way to instruments for analytical flow cytometry, which in some cases must be maintained in appropriate biosafety containment conditions. The core has three specific aims: 1) Provide and maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards; 2) Provide technical assistance in the performance and interpretation of established techniques involving flow cytometry; 3) Introduce new and update existing technologies to maintain cutting edge capabilities. The core facility supported by the P01 has full responsibility for maintaining one analytical flow cytometer, and contributes to the maintenance and operation of one high speed cell sorter. Access through this core to FACS analysis and sorting techniques under Biosafety Level (BSL)-2 and BSL-3 conditions has played an essential role in research funded by this P01 grant during the past 5 years, and will continue to do so into the next funding period. The high cost of the instrumentation and the need for skilled personnel makes the establishment of a core facility for flow cytometry the only practical approach to management of these resources. In addition, this core facility provides the only possibility for the P01 investigators to do flow cytometry in a BSL-3 environment within the institution, and thus is a crucial facility for some of the studies that are ongoing or planned within the context of the program. The core also provides an environment in which biosafety containment procedures can be strictly overseen and enforced, thus ensuring the safety of personnel using flow cytometry techniques in their work with potentially dangerous infectious agents. The analytical capabilities of the core allow the program investigators to identify and characterize cell populations of interest in a wide range of studies related to mycobacterial pathogenesis and the host immune response.

流式细胞术是分析细胞表型和分离特定细胞群的最广泛使用和不可缺少的技术之一。由P01支持的流式细胞术核心资源以具有成本效益的方式为该计划的研究人员提供更多的流式细胞术分析仪器，在某些情况下，这些仪器必须保持在适当的生物安全防护条件下。该核心有三个具体目标：1）提供和维护流式细胞术研究所需的必要研究基础设施，并配备适当的生物安全防护措施; 2）在流式细胞术既定技术的性能和解释方面提供技术援助; 3）引进新技术并更新现有技术，以保持尖端能力。由P01支持的核心设施完全负责维护一台分析流式细胞仪，并负责维护和操作一台高速细胞分选机。在过去5年中，在生物安全等级（BSL）-2和BSL-3条件下，通过该核心获得FACS分析和分选技术在P01资助的研究中发挥了重要作用，并将在下一个资助期继续发挥作用。仪器的高成本和对熟练人员的需求使得建立流式细胞术的核心设施成为管理这些资源的唯一实用方法。此外，该核心设施为P01研究者在机构内的BSL-3环境中进行流式细胞术提供了唯一的可能性，因此是该项目背景下正在进行或计划进行的一些研究的关键设施。该核心还提供了一个环境，在其中可以严格监督和执行生物安全遏制程序，从而确保使用流式细胞术技术的人员在其工作中与潜在危险的传染性病原体的安全。核心的分析能力使项目研究人员能够在与分枝杆菌发病机制和宿主免疫反应相关的广泛研究中识别和表征感兴趣的细胞群。