FLOW CYTOMETRY SHARED RESOURCE

流式细胞术共享资源为AECC研究人员提供了方便和实惠的访问 流式细胞术中广泛的重要技术，并支持许多研究 其依赖于高速荧光激活细胞分选（FACS）和先进的多参数 荧光分析除了提供先进的流式细胞仪设备外， 提供技术专长和培训，指导AECC调查人员及其工作人员应用 基本和先进的流式细胞术技术。该设施还组织和支持一个计划， 关于新技术的研讨会，向AECC调查人员介绍新出现的流式细胞仪应用 可以促进他们的研究。该共享资源的主要用途是多参数分析， 基于用荧光染料缀合的抗体或其它标记的细胞群的高速分选 荧光蛋白或化学染料试剂。对许多调查人员进行了使用设施的培训， 分析仪器的使用已经形成了一个非常庞大和训练有素的用户群。2005-2006年期间，该设施 为超过275名注册用户提供了服务，其中包括教师、博士后研究员、学生和 来自78个不同实验室的技术人员。AECC成员是其中45个项目的主要研究人员 2005-2006两年期，所有设施服务使用总时数的三分之二是由于 AECC研究人员的实验室。这些调查人员每天七天， 该设施的分析仪，其中包括Becton-Dickinson FACScan（单激光，3色），FACSCalibur (two激光，4色）和LSR-II（4激光，12色）仪器。标准荧光显微镜和 具有用于处理FACS数据的广泛软件库的计算机工作站也是常规的 available.使用该设施的3激光、8色Dako-Cytomation MoFlo分选机进行高速细胞分选， 由经过培训的设施工作人员在工作日的正常时间内专门执行。此共享 资源具有许多增值功能，并得到大量的机构支持， 协助采购最先进的仪器和一流的流式细胞术专家。

The Flow Cytometry Shared Resource provides AECC investigators with convenient and affordable access to a broad spectrum of important technologies in flow cytometry, and supports numerous research studies that rely on high speed fluorescence activated cell sorting (FACS) and advanced multiparameter fluorescence analysis. In addition to providing advanced flow cytometry equipment, the shared resource provides technical expertise and training to guide AECC investigators and their staff in the application of basic and advanced flow cytometry techniques. The facility also organizes and supports a program of seminars on new technologies that inform AECC investigators about newly emerging applications of FACS that can enhance their research. The major usage of this shared resource is for multiparameter analysis and high speed sorting of cell populations based on labeling with fluorochrome-conjugated antibodies or other fluorescent protein or chemical dye reagents. Training of many investigators in the use of the facility's analytical instruments has led to a very large and well-trained user base. During 2005-2006, the facility provided services to more than 275 registered users, consisting of faculty, postdoctoral fellows, students and technicians from 78 different laboratories. AECC members were principal investigators of 45 of these laboratories, and two thirds of total hours of usage of all facility services in 2005-2006 was attributable to the laboratories of AECC investigators. These investigators have access around the clock and seven days per week to the facility's analyzers, which include Becton-Dickinson FACScan (one laser, 3-color), FACSCalibur (two laser, 4-color), and LSR-II (4 laser, 12 color) instruments. Standard fluorescent microscopes and computer work stations with an extensive software library for processing of FACS data are also routinely available. High speed cell sorting with the facility's 3-laser, 8-color Dako-Cytomation MoFlo sorter is performed exclusively by the trained facility staff during regularly scheduled weekday hours. This shared resource has numerous value-added features, and receives substantial institutional support that has greatly assisted the acquisition of state-of-the-art instrumentation and a superb staff of flow cytometry experts.