Flow Cytometery and Cell Sorting Core

核心B -流式细胞术和细胞分选核心（核心负责人：Porcelli）项目负责人：Backer，Jonathan M. 摘要 流式细胞术分析和细胞分选核心（核心B）将提供设备和专业知识 用于单细胞水平分析和分离的各种先进的基于流式细胞术的技术。的 核心将支持和维护一系列用于分析和分类应用的先进仪器， 积极指导项目研究者将其应用于癌症转移的研究。这些 方法学将用于鉴定、分离和详细分析感兴趣的细胞类型， 与计划项目目标的相关性。核心资源将广泛用于所有项目 包括该计划，以及他们在该结构下的组织和支持将提供该计划 调查人员以具有成本效益的方式获得访问和技术支持。核心的关键人员将 致力于直接协助项目PI开发和实施单一 细胞分析技术相关的项目，并协助他们在创新的新方法， 核心技术通过为基于流式细胞术的单细胞组合提供组织核， 分析和隔离方法，核心B将创建一个基础设施和环境， 不断发展的技术，以满足PPG研究人员的需求。所有必要的设备和专业知识 这一核心所需的资源已经到位，并可在我们的机构。关键人员配备必要的 背景和专业知识已经从现有的教师和工作人员中招募，这些人已经 已指定适当的工作级别，以确保核心B的正常运行。虽然一些 与核心有关的设备和人员实际上都安置在我们的 NCI CCSG（P30）资助的流式细胞仪核心设施，核心B的创建对于成功 实现PPG的目标，因为它将使这些技术的实施， 和创新的应用，专门解决癌症转移的研究，形成的主题， 当前的应用程序。 PHS 398/2590（Rev.11/07）

Core B – Flow Cytometry and Cell Sorting Core (Core Lead: Porcelli) Program Lead: Backer, Jonathan M. ABSTRACT The Flow Cytometry Analysis and Cell Sorting Core (Core B) will provide access to equipment and expertise for a variety of advanced flow cytometry-based technologies for single cell level analysis and isolation. The core will support and maintain a range of advanced instruments for analytic and sorting applications, and will actively guide the program investigators in applying these to their studies on cancer metastasis. These methodologies will be applied for the identification, isolation and detailed profiling of cell types of interest and relevance to the goals of the program project. The resources of the core will be used extensively in all projects comprising this program, and their organization and support under this structure will provide the Program investigators with access and technical support in a cost effective way. The key personnel of the core will have effort dedicated to directly assisting the program PIs in the development and implementation of single cell analysis techniques relevant to their projects, and assist them in innovating new approaches using the core technologies. By providing an organizing nucleus for a portfolio of flow cytometry-based single cell analysis and isolation methods, Core B will create an infrastructure and environment for adapting these evolving technologies to the needs of the PPG investigators. All of the essential equipment and expertise required for this core are already in place and available at our institution. Key personnel with the necessary background and expertise have been recruited from existing faculty and staff, and these individuals have been designated appropriate levels of effort to ensure proper functioning of Core B. While some of the equipment and personnel associated with the core are physically housed and partially supported within our NCI CCSG (P30) funded Flow Cytometry Core Facility, the creation of Core B is essential to the successful accomplishment of the goals of the PPG, as it will enable the implementation of these technologies for new and innovative applications that specifically address the studies of cancer metastasis that form the theme of the current program application. PHS 398/2590 (Rev. 11/07) Page