Digital Extra Parameter (DxP8)

描述(由申请人提供)：我们正在申请资金，为现有的BD Biosciences FACSCalibur分析型流式细胞仪进行Cytek Development DXP 8色升级。需要升级的仪器是微生物学和免疫学系于2005年购买的共享核心设备。它目前拥有标准的2激光和4色配置，因此允许对相对有限范围的荧光染料和荧光蛋白质进行最多4种彩色荧光分析。该仪器得到了该部许多实验室的广泛使用，并得到了良好的维护和良好的工作状态。然而，由于许多部门调查人员所做的FACS分析越来越复杂，这种相对简单的流式细胞仪已不能满足当前的需要。解决此问题的一个经济高效的解决方案是购买并安装此应用程序要求的Cytek Digital 8色彩升级。Cytek Development是一家成熟的公司，专门从事流式细胞仪的维修、维护、翻新和升级。我们在这家公司拥有超过5年的丰富经验，我们对他们的产品和服务的经验使我们相信，拟议中的8色升级将是一个具有成本效益的解决方案，可以满足我们目前对更先进的流式细胞仪的需求。NIH资助的微生物学和免疫学系至少有13名教员已被确定为该设备的使用者，很可能还会从该系16名初级教员和19名中级教员运营的实验室中确定其他用户。该设备的使用和维护将由该应用程序的PI Steven A.Porcelli博士监督，他是微生物学和免疫学系教授，也是爱因斯坦园区所有共享的流式细胞仪资源的总体科学总监。一名在维护和操作FACSCalibur流式细胞仪方面有经验的技术人员和一些专家顾问可以确保升级后的FACSCalibur的功能将得到充分利用。DxP8 8颜色升级将为NIH资助的一大批微生物学家和免疫学家提供先进的流式细胞仪技术，这些技术对广泛的基础和翻译研究项目至关重要。

DESCRIPTION (provided by applicant): We are requesting funds for a Cytek Development DxP 8-color upgrade for an existing BD Biosciences FACSCalibur analytical flow cytometer. The instrument to be upgraded is shared core equipment that was purchased by the Department of Microbiology and Immunology in 2005. It currently has the standard 2 laser and 4 color configurations, and thus allows up to 4 color fluorescence analyses with a relatively restricted range of fluorochromes and fluorescent proteins. This instrument is used extensively by many of the laboratories in the Department, and has been well maintained and is in excellent working order. However, because of the increasing complexity of FACS analyses done by many of the Department's investigators, this relatively simple flow cytometer is no longer able to meet current needs. A cost-effective solution to this problem is the purchase and installation of the Cytek digital 8 color upgrade requested by this application. Cytek Development is a well established company that specializes in the repair, maintenance, refurbishing and upgrading of flow cytometers. We have extensive experience with this company going back more than 5 years, and our experience with their products and services makes us confident that the proposed 8 color upgrade will be a cost- effective solution to our current need for a more advanced flow cytometer. At least 13 NIH funded faculty in the Department of Microbiology and Immunology have been identified as users for this equipment, and it is likely that additional users will be identified from laboratories run by the Department's 16 primary and 19 secondary faculty members. The use and maintenance of this equipment will be supervised by the PI of this application, Dr. Steven A. Porcelli, who is Professor in the Department of Microbiology and Immunology and overall Scientific Director of all of the shared flow cytometry resources on the Einstein campus. A technician with experience in maintaining and operating FACSCalibur flow cytometers and a number of expert consultants are available to ensure that the features of the upgraded FACSCalibur will be used to full advantage. The DxP8 8 color upgrade will provide a large group of NIH-funded microbiologists and immunologists with access to advanced flow cytometry techniques that are critically important to a wide range of basic and translational research projects.