DRILL: Droplet Desolvation and Ion Transmission Interface for Mass Spectrometry

DRILL：用于质谱分析的液滴去溶剂化和离子传输接口

• 批准号: 8927665
• 负责人: ANDREI G FEDOROV
• 金额: $ 54.56万
• 依托单位: GEORGIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-15 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8927665
• 关键词: Address; Atmospheric Pressure; Biochemical; Biological; Biological Markers; Biological Preservation; Biomedical Engineering; Cancer Model; Charge; Chickens; Cleaved cell; Clinical; Collection; Complex; Complex Mixtures; Coupled; Deposition; Detection; Development; Devices; Disease; Drug Design; Educational workshop; Effectiveness; Electrodes; Electrospray Ionization; Engineering; Evaluation; Gases; Generations; Geometry; Goals; Guidelines; Health; Health Sciences; Heating; Infusion procedures; Injection of therapeutic agent; Interdisciplinary Study; Ion Transport; Ions; Knowledge; Lasers; Link; Location; Locomotion; Malignant neoplasm of ovary; Maps; Mass Spectrum Analysis; Methodology; Methods; Microdissection; Modeling; Molecular; Noise; Ovarian Tissue; Paper; Performance; Physics; Plasma; Plasma Proteins; Polysaccharides; Production; Protein Analysis; Proteins; Proteomics; Reproducibility; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Science; Scientist; Sensitivity and Specificity; Side; Signal Transduction; Site; Solvents; Special Event; Stream; System; Technology; Thermometers; Tissue Sample; Tissues; Training; Width; Work; Yeasts; base; design; drug development; engineering design; hydrophilicity; improved; innovation; insight; interdisciplinary collaboration; ion source; ionization; ionization technique; mass analyzer; meetings; member; metabolomics; model design; neoplastic cell; novel; operation; particle; research study; sensor; simulation; sound; tool; transmission process

DESCRIPTION (provided by applicant): The overarching goal of this proposal is to develop a novel atmospheric pressure droplet desolvation/ ion collection interface for efficient delivery of softly ionized biomolecules to mass analyzers, which will dramatically improve sensitivity and dynamic range of mass spectrometry (MS) for complex biochemical samples. Such an interface is a critical enabling technology for raising MS capability to the next level, and to further advance this key tool in health related sciences, including applications to proteomics, metabolomics, biomarker discovery and drug development. An interdisciplinary research team comprised of a bioengineer, an analytical chemist, and a proteomics scientist will develop, demonstrate and optimize the novel interface system, DRILL (DRy Ion Localization and Locomotion), for ESI LC-MS workflows with a number of unique capabilities, using a multifaceted approach that combines innovative engineering design, supported by powerful electrohydrodynamics analysis, simulation and experiments, advanced DOE (design of experiment) analytical characterization methodology, and state-of-the-art applications to challenging proteomics problems. With an optimally-designed DRILL desolvation and ion transport interface, we aim to eliminate ion losses due to incomplete desolvation (including signal suppression) and inefficient ion collection, and to enable a dramatic improvement in sensitivity and dynamic range of detection as compared to the current state of the art. The research team members are well situated to meet the scientific and engineering challenges associated with addressing the need for an improved MS interface, with a track record of successful research on ion sources, bio-analytical devices and sensors, mass spectrometry, proteomics and interdisciplinary collaboration. As a result of this work, the research team will have completed all steps necessary to make the proposed DRILL system a widely applicable tool for biological and clinical researchers, aiming to improve current MS performance and to develop new MS applications for both top-down and bottom-up proteomics. Broad dissemination to enhance scientific and technological understanding will be achieved through several activities. In particular, the research results will be communicated through technical papers and presentations in scientific forums, developing an online resource summarizing the design and operation guidelines for DRILL device operation and enabled new LC-MS workflows, as well as special events (hands-on training workshops for clinical practitioners) that the research team plans to organize.

描述（由申请人提供）：该提案的总体目标是开发一种新型的大气压力液滴脱溶剂/离子收集界面，以有效地传递柔和的电离生物分子向大众分析仪，这将极大地提高质谱和动态范围的质谱范围（MS），以使复杂的生物化学样品的质谱范围（MS）的动态范围。这样的界面是将MS能力提高到下一个水平的重要促进技术，并进一步推进了与健康相关的科学的关键工具，包括应用于蛋白质组学，代谢组学，生物标志物发现和药物开发的应用。一个由生物工程师，分析化学家和蛋白质组学科学家组成的跨学科研究团队将使用多种功能来开发，演示和优化新型界面系统，钻探（干离子定位和运动），以使用多进程的功能来组合多个独特的实验性，以构建功能，以构建功能，以构建功能，以构建功能，以构建功能，以实现精力，并将其构建强大的expentim equartimimentimectimentimentimentimentimentimed Elementimed andimentimectimentimentimentimentimentimentimentimentimed Electimed Drying Drying Drownimed，该功能是强有力的设计，该设计均具有强大的设计，该设计均应设计，并具有功能。先进的DOE（实验设计）分析特征方法以及对挑战蛋白质组学问题的最新应用。借助最佳设计的钻凹和离子传输界面，我们旨在消除由于不完全脱溶液（包括信号抑制）和效率低下的离子收集而导致的离子损失，并与现有现状相比，敏感性和动态检测的敏感性和动态检测范围可显着改善。研究小组成员的位置良好，可以应对与改进MS接口的需求相关的科学和工程挑战，并具有成功研究有关离子源，生物分析设备和传感器，质谱，蛋白质组学和跨学科协作的记录。这项工作的结果是，研究团队将完成所有必要的步骤，以使所提出的钻探系统成为生物学和临床研究人员广泛适用的工具，旨在提高当前的MS性能并为自上而下和自下而上的蛋白质组学开发新的MS应用程序。通过多种活动将实现广泛的传播以增强科学和技术理解。特别是，研究结果将通过科学论坛的技术论文和演示进行传达，开发在线资源，总结了钻井设备操作的设计和操作指南，并使新的LC-MS工作流程以及研究团队计划组织的特殊活动（为临床实践者提供的动手培训研讨会）。