Role of two novel genes in IL-23 production and Th17-mediated pathogenesis in SLE

描述（由申请人提供）：在系统性红斑狼疮（SLE）中，CD4 T细胞通过提供共刺激信号和细胞因子是B细胞依赖性自身抗体反应的关键驱动因素。快速清除凋亡细胞（ACs）被认为是解决炎症和预防自身免疫性疾病的核心。目前的理论认为，吞噬细胞摄取ac后，通过识别ac特异性分子，诱导免疫调节细胞因子转化生长因子- β (TGF-b)的合成，导致免疫抑制状态的产生和预防。源自ACs的持续性自身抗原是SLE发病的主要原因。实验研究表明，树突状细胞（dc）可以在SLE中加工和呈递来自ACs的自身抗原。与此相反，我们观察到，在缺乏微生物刺激的情况下，人和小鼠髓系dc和吞噬ACs的巨噬细胞产生了大量的IL-23、IL-6和TGF-b，这些细胞因子是Th17细胞发育的必需细胞因子，Th17细胞与SLE的器官特异性发病密切相关。我们进一步发现了两个新的转录因子，LRRC16B和FLJ44967，对于暴露于ACs的吞噬细胞诱导IL-23至关重要和直接。我们推测，在sle易感个体/宿主中，吞噬细胞清除ACs的过程中存在IL-23/ th17生成通路的过度激活，这种激活依赖于LRRC16B和FLJ44967的表达和活性。我们建议：(1)阐明LRRC16B和FLJ44967在专业吞噬细胞/APCs中响应ACs诱导表达的细胞和分子机制；(2)研究LRRC16B和FLJ44967在狼疮和非自身免疫性DWF1小鼠BWF1模型中吞噬ACs的髓系dc在Th17发展和SLE发病中的作用。这项研究将使我们更深入地了解Th17在稳态生理学和自身免疫性疾病的病理生理学中的发展和活动的基础。

DESCRIPTION (provided by applicant): In systemic lupus erythematosus (SLE), CD4 T cells are critical drivers of the B cell- dependent autoantibody responses via provision of co-stimulatory signals and cytokines. Rapid removal of apoptotic cells (ACs) is considered central to the resolution of inflammation and in preventing autoimmune disease. Current dogma states that uptake of ACs by phagocytes induces synthesis of the immunoregulatory cytokine transforming growth factor- beta (TGF-b) through recognition of AC-specific molecules, resulting in the generation of an immunosuppressive state and prevention. Persistent self-antigens derived from ACs is a major causative event in the etiopathogenesis of SLE. Experimental studies have shown that dendritic cells (DCs) can process and present autoantigens from ACs in SLE. In contrast to the dogma, we observed that in the absence of microbial stimuli, human and mouse myeloid DCs and macrophages engulfing ACs produced substantial amounts of IL-23, IL-6, and TGF-b, the essential cytokines for the development of Th17 cells that have been strongly implicated in organ-specific pathogenesis of SLE. We further identified two novel transcription factors, LRRC16B and FLJ44967, as critically important and direct for the induction of IL-23 in phagocytes exposed to ACs. We hypothesize that in SLE-susceptible individuals/hosts, there is an overactivation of the IL-23/Th17-generating pathway in the process of clearance of ACs by phagocytes in a manner dependent on the expression and activities of LRRC16B and FLJ44967. We propose to: (1) Elucidate the cellular and molecular mechanisms whereby expression of LRRC16B and FLJ44967 is induced in professional phagocytes/APCs in response to ACs; (2) Investigate the role of LRRC16B and FLJ44967 in myeloid DCs engulfing ACs in Th17 development and SLE pathogenesis in the BWF1 model of lupus and non-autoimmune DWF1 mice. This investigation will lead to greater insights into the basis of Th17 development and activities in steady state physiology, and in the pathophysiology of autoimmune disorders.