A Bioengineerable Self-adjuvating Nanoparticle for Antigen Delivery

用于抗原递送的生物工程自辅助纳米颗粒

• 批准号: 8665391
• 负责人: SHILADITYA DASSARMA
• 金额: $ 7.68万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-25 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8665391
• 关键词: Acute; Address; Adjuvant; Animals; Antigens; Archaea; Area; Attenuated; Bacterial Antigens; Baltimore; Bioinformatics; Biological; Biomedical Engineering; Biotechnology; Bone Marrow; Caliber; Cells; Cessation of life; Child; Code; Collaborations; Communicable Diseases; Country; Cytolysis; Data; Developing Countries; Development; Disease; Disease Outbreaks; Drug Formulations; Effectiveness; Evaluation; Fever; Food; Foundations; Gallbladder; Gases; Gene Cluster; Genetic Engineering; Goals; Gut associated lymphoid tissue; Human; Immune response; Immunization; Immunology; Inbred BALB C Mice; Individual; Infant; Infection; Institutes; International; Lead; Length; Letters; Licensing; Life; Liver; Local Anti-Infective Agents; Love; Maryland; Membrane; Methods; Microbiology; Morbidity - disease rate; Mus; Oral; Oral Administration; Organism; Paratyphoid Fever; Plasmids; Prevention; Property; Proteins; Recombinants; Refrigeration; Route; Safety; Salmonella; Salmonella Vaccines; Salmonella enterica; Salmonella typhi; Sampling; Sanitation; Science; Scientist; Shapes; Sodium Chloride; Spleen; Surface; System; Temperature; Testing; Typhoid Fever; Universities; Vaccine Antigen; Vaccines; Vesicle; Viral; Virulent; Water; Work; base; comparative; efficacy testing; gas vesicle protein; immunogenic; improved; intraperitoneal; mortality; mouse model; nanoparticle; novel; novel strategies; oral vaccine; particle; pathogen; polypeptide; protective efficacy; public health relevance; response; vaccine candidate; vaccine delivery; vaccinology; wasting

DESCRIPTION (provided by applicant): A unique vaccine platform employs novel bioengineerable nanoparticles, Haloarchaeal gas vesicles (HGVs), as an adjuvant and antigen delivery system that is inexpensive, effective, and shelf-stable (requiring no refrigeration). When administered to animals, HGV nanoparticles with surface-displayed antigens produce strong long-lived immune responses. In previous work, antigens from bacterial and viral pathogens were expressed as a fusion to one of the HGV protein components and in all cases the recombinant HGVs were highly immunogenic in mice. We plan to test the effectiveness of HGVs as a vaccine vehicle against Salmonella enterica serovar Typhi, the causative agent of typhoid fever. These human pathogens remain important causes of morbidity and mortality in many countries as a result of poor sanitation, and water and food contaminated by human fecal waste. Our goal is to progress toward the development of a safe and inexpensive oral vaccine against invasive Salmonella disease that can be used in both the developing and developed world. We propose to: (a) produce multiple Salmonella protective antigens displayed on HGV nanoparticles; and (b) deliver the bioengineered HGV-vaccine antigen nanoparticles and determine their protective efficacy in a mouse model. We will also perform more in-depth immunological analyses and test protection using combinations of HGV formulations. These studies will lay the foundation for subsequent evaluation of this approach in humans as well as customize the system for prevention of other diseases. The development of a safe, oral adjuvant and vaccine delivery vehicle which is inexpensive, effective, and shelf-stable should lead to a global revolution in the prevention of infectious diseases.

描述（由申请人提供）：一种独特的疫苗平台采用新型生物工程纳米颗粒，盐古菌气体囊泡（HGVs）作为佐剂和抗原递送系统，价格低廉，有效且货架稳定（不需要冷藏）。当

项目成果

Gas Vesicle Nanoparticles for Antigen Display.

• DOI: 10.3390/vaccines3030686
• 发表时间: 2015-09-07
• 期刊: Vaccines
• 影响因子: 7.8
• 作者: DasSarma S;DasSarma P
• 通讯作者: DasSarma P

Halophiles and their enzymes: negativity put to good use.

• DOI: 10.1016/j.mib.2015.05.009
• 发表时间: 2015-06
• 期刊: Current opinion in microbiology
• 影响因子: 5.4
• 作者: DasSarma S;DasSarma P
• 通讯作者: DasSarma P

Haloarchaeal gas vesicle nanoparticles displaying Salmonella antigens as a novel approach to vaccine development.

• DOI: 10.1016/j.provac.2015.05.003
• 发表时间: 2015
• 期刊: Procedia in vaccinology
• 作者: DasSarma P;Negi VD;Balakrishnan A;Kim JM;Karan R;Chakravortty D;DasSarma S
• 通讯作者: DasSarma S

An improved genetic system for bioengineering buoyant gas vesicle nanoparticles from Haloarchaea.

• DOI: 10.1186/1472-6750-13-112
• 发表时间: 2013-12-21
• 期刊: BMC biotechnology
• 影响因子: 3.5
• 作者: DasSarma S;Karan R;DasSarma P;Barnes S;Ekulona F;Smith B
• 通讯作者: Smith B

Haloarchaeal gas vesicle nanoparticles displaying Salmonella SopB antigen reduce bacterial burden when administered with live attenuated bacteria.

• DOI: 10.1016/j.vaccine.2014.06.021
• 发表时间: 2014-07-31
• 期刊: Vaccine
• 影响因子: 5.5
• 作者: DasSarma P;Negi VD;Balakrishnan A;Karan R;Barnes S;Ekulona F;Chakravortty D;DasSarma S
• 通讯作者: DasSarma S