Diversity of Lactobacilli Associated with Severe Early Childhood Caries

与严重儿童早期龋齿相关的乳酸菌多样性

• 批准号: 8718793
• 负责人: PAGE W CAUFIELD
• 金额: $ 63.15万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-22 至 2016-07-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8718793
• 关键词: Adherence; Adult; Area; Bacteria; Benchmarking; Carbohydrates; Categories; Characteristics; Child; Code; Collection; Coupled; Databases; Dental; Dental Plaque; Dental caries; Diet; Elements; Environment; Functional RNA; Future; Genes; Genetic; Genetic Variation; Genome; Genomic Islands; Genomics; Genotype; Goals; Habitats; Health; High Prevalence; Individual; Lactobacillus; Lesion; Link; Measures; Methods; Microbe; Mobile Genetic Elements; Natural History; Oral cavity; Pathway interactions; Phylogeny; Plasmids; Play; Population; Probiotics; Process; Property; Publications; Risk; Risk Assessment; Role; Saliva; Sampling; Severities; Site; Source; Specificity; Streptococcus mutans; Surveys; Taxonomy; Techniques; Technology; Testing; Virulence; White Spots; base; cariogenic bacteria; cohort; comparative genomics; comparison group; early childhood; food environment; genetic element; genome sequencing; insight; meetings; member; next generation sequencing; rRNA Genes; reconstruction; trait

DESCRIPTION (provided by applicant): The long-term objective of this application is to uncover the characteristics of a group of bacteria called the lactobacilli (Lb) that play a key role in the progression and severity of dental caries in both children and adults. For almost a century, the link between caries and Lb was known, but further progress in delineating which of the more than 100 species of lactobacilli were responsible for dental caries remained elusive. Recent advances in genetic methods and the publication of a dozen Lb genomes now make it possible to gain important insight into the sub-group of Lb members and the cariogenic traits responsible for caries. These goals will be met by two specific aims. Aim 1 is to conduct a survey of the Lb populations in three groups of children, those with severe early childhood caries (S-ECC) and two comparison groups, a caries free (CF) and a moderately active caries (white spot lesion, WSL) group. We will accomplish these aims by both culture-independent and culture-dependent methods to obtain estimates of abundance and diversity of the Lb populations. We will also identify individual Lb genotypes found in the three groups by AP-PCR. Lb species identification of unique genotypes will be accomplished by 16S rRNA gene sequencing to obtain a collection of isolates that represent the different species/genotypes that characterize the S-ECC, WSL, and CF habitat or niche. Aim 2 is to identify genetic attributes associated with the caries-niche. A combination of whole-genome sequencing and comparative genomics applied to the isolates derived from Aim 1, coupled with non-oral Lb genome sequences available in public and private database, will result in the identification of genetic signatures unique to the caries/caries-free niche. With this information, clinicians will be able to analyze a bacterial sample from the mouth and determine whether the Lb present are cariogenic or not. This, in turn, will give clinicians an estimation of risk for, and severity of, dental caries. An important spin off to these findings will pave the way toward assessing the cariogenic potential of Lb proposed as health-associated probiotics.

描述（由申请人提供）：本申请的长期目标是揭示一组称为乳酸杆菌 (Lb) 的细菌的特征，这些细菌在儿童和成人龋齿的进展和严重程度中发挥着关键作用。近一个世纪以来，人们都知道龋齿和乳酸菌之间的联系，但在确定 100 多种乳酸杆菌中哪一种是导致龋齿的原因方面仍难以取得进一步进展。遗传方法的最新进展和十几个 Lb 基因组的公布现在使得人们有可能深入了解 Lb 成员的亚组和导致龋齿的致龋性状。这些目标将通过两个具体目标来实现。目标 1 是对三组儿童的 Lb 人群进行调查，即患有严重幼儿期龋齿 (S-ECC) 的儿童和两个对照组：无龋齿组 (CF) 和中度活动性龋齿组（白斑病变，WSL）。我们将通过独立于文化和文化依赖的方法来实现这些目标，以获得 Lb 种群丰度和多样性的估计。我们还将通过 AP-PCR 鉴定三组中发现的个体 Lb 基因型。独特基因型的 Lb 物种鉴定将通过 16S rRNA 基因测序来完成，以获得代表 S-ECC、WSL 和 CF 栖息地或生态位特征的不同物种/基因型的分离株集合。目标 2 是确定与龋齿生态位相关的遗传属性。将全基因组测序和比较基因组学应用于源自 Aim 1 的分离株，再加上公共和私人数据库中提供的非口腔 Lb 基因组序列，将导致识别龋齿/无龋齿生态位特有的遗传特征。有了这些信息，临床医生将能够分析口腔中的细菌样本，并确定其中的 Lb 是否致龋。反过来，这将使临床医生能够估计龋齿的风险和严重程度。这些发现的一个重要副产品将为评估 Lb 作为健康相关益生菌的致龋潜力铺平道路。

项目成果

Characterizing Diversity of Lactobacilli Associated with Severe Early Childhood Caries: A Study Protocol.

• DOI: 10.4236/aim.2015.51002
• 发表时间: 2015-01-01
• 期刊: Advances in microbiology
• 作者: Li Y;Argimón S;Schön CN;Saraithong P;Caufield PW
• 通讯作者: Caufield PW

Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera.

通过 213 种菌株和相关属的比较基因组学扩大乳酸杆菌的生物技术潜力

• DOI: 10.1038/ncomms9322
• 发表时间: 2015-09-29
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Sun Z;Harris HM;McCann A;Guo C;Argimón S;Zhang W;Yang X;Jeffery IB;Cooney JC;Kagawa TF;Liu W;Song Y;Salvetti E;Wrobel A;Rasinkangas P;Parkhill J;Rea MC;O'Sullivan O;Ritari J;Douillard FP;Paul Ross R;Yang R;Briner AE;Felis GE;de Vos WM;Barrangou R;Klaenhammer TR;Caufield PW;Cui Y;Zhang H;O'Toole PW
• 通讯作者: O'Toole PW