Microbial Diversity and Genetic Characterization of Cariogenic Biofilms

致龋生物膜的微生物多样性和遗传特征

• 批准号: 7372919
• 负责人: PAGE W CAUFIELD
• 金额: $ 36.68万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-15 至 2012-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7372919
• 关键词: Accounting; Acute; Address; Affect; Algorithms; Alleles; Artificial Intelligence; Biological Markers; Categories; Causations; Child; Childhood; Class; Classification; Coupling; DNA; Dental; Dental caries; Diagnostic; Diagnostic tests; Disease; Dot Immunoblotting; Effectiveness; Future; Genes; Genetic; Genetic Determinism; Genetic Markers; Genetic Variation; Genomic Islands; Genomics; Health; Hispanics; Infant; Knowledge; Lead; Literature; Machine Learning; Microbe; Microbial Biofilms; Modeling; Molecular; Mothers; Numbers; Pathogenesis; Personal Satisfaction; Polymerase Chain Reaction; Population; Population Heterogeneity; Predictive Value; Prevention strategy; Process; Programmed Learning; Public Health; Pulsed-Field Gel Electrophoresis; Purpose; Research; Research Personnel; Risk; Score; Series; Severity of illness; Streptococcus mutans; Surveys; Techniques; Testing; Thinking; Virulence; Virulence Factors; base; early childhood; experience; genetic element; microbial; programs; research study; tool

DESCRIPTION (provided by applicant): The proposed studies are anticipated to yield important new information as to the presence of specific genetic determinants found in Streptococcus mutans, a microbe strongly associated with a severe dental caries in affecting young children called severe early childhood caries or S-ECC. The long-term benefit of such information should lead researchers toward devising both diagnostic and preventive strategies against dental though better understanding its microbial causation. Using a powerful technique of suppressive subtraction DNA hybridization, the first specific aim is to test the hypothesis that strains of S. mutans found in S-ECC children differ in genetic composition compared to strains found in caries-free (CF) children. Unique genetic loci that are specific to S-ECC strains will be identified and characterized. Some of these genetic loci will prove informative as biomarkers for health or disease. Using the power of multiple test analyses derived artificial intelligence programming; biomarkers will be used as the basis for a modeling algorithm capable of classifying strains of S. mutans in one of two categories (S-ECC or CF). The second aim of this study is to survey strains of S. mutans associated with either S-ECC or CF status for known or suspected genetic elements thought to influence the pathogenesis or virulence of S. mutans. The third aim is to survey a larger, racial/ethnical diverse population of S-ECC or CF children for the presence or absence of the biomarkers identified in the first two aims. It is anticipated that this research will yield genetic markers for disease capable of providing clinicians with a diagnostic test that can assess risk for developing S-ECC. 7.

描述（由申请人提供）：拟议的研究预计将产生关于变形链球菌中是否存在特定遗传决定因素的重要新信息，变形链球菌是一种与影响幼儿的严重龋齿（称为严重儿童早期龋齿或 S-ECC）密切相关的微生物。这些信息的长期利益应该引导研究人员通过更好地了解其微生物原因来设计针对牙科的诊断和预防策略。使用强大的抑制消减 DNA 杂交技术，第一个具体目标是检验以下假设：S-ECC 儿童中发现的变形链球菌菌株与无龋齿 (CF) 儿童中发现的菌株在遗传组成上存在差异。 S-ECC 菌株特有的独特遗传位点将被鉴定和表征。其中一些基因位点将作为健康或疾病的生物标志物提供丰富的信息。利用多重测试分析的力量衍生人工智能编程；生物标志物将用作建模算法的基础，该算法能够将变形链球菌菌株分类为两个类别（S-ECC 或 CF）之一。本研究的第二个目的是调查与 S-ECC 或 CF 状态相关的变形链球菌菌株，以了解被认为影响变形链球菌发病机制或毒力的已知或可疑遗传元件。第三个目标是调查更大的、种族/民族多样化的 S-ECC 或 CF 儿童群体，以确定是否存在前两个目标中确定的生物标志物。预计这项研究将产生疾病的遗传标记，能够为临床医生提供诊断测试，以评估发生 S-ECC 的风险。 7.