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Microbial Diversity and Genetic Characterization of Cariogenic Biofilms

致龋生物膜的微生物多样性和遗传特征

基本信息

批准号：
8205040
负责人：
PAGE W CAUFIELD
金额：
$ 36.41万
依托单位：
NEW YORK UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-06-15 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8205040
关键词：
Accounting Acute Address Affect Algorithms Alleles Artificial Intelligence Biological Markers Categories Child Childhood Classification Coupling DNA Dental Dental caries Diagnostic Diagnostic tests Disease Dot Immunoblotting Effectiveness Epidemiology Etiology Future Genes Genetic Genetic Determinism Genetic Markers Genetic Variation Genomic Islands Genomics Health Hispanics Infant Knowledge Lead Literature Machine Learning Microbe Microbial Biofilms Modeling Molecular Mothers Pathogenesis Population Population Heterogeneity Predictive Value Prevention strategy Process Programmed Learning Public Health Pulsed-Field Gel Electrophoresis Research Research Personnel Risk SHH gene Series Severity of illness Streptococcus mutans Surveys Techniques Testing Virulence Virulence Factors base early childhood experience genetic element microbial programs research study tool

项目摘要

DESCRIPTION (provided by applicant): The proposed studies are anticipated to yield important new information as to the presence of specific genetic determinants found in Streptococcus mutans, a microbe strongly associated with a severe dental caries in affecting young children called severe early childhood caries or S-ECC. The long-term benefit of such information should lead researchers toward devising both diagnostic and preventive strategies against dental though better understanding its microbial causation. Using a powerful technique of suppressive subtraction DNA hybridization, the first specific aim is to test the hypothesis that strains of S. mutans found in S-ECC children differ in genetic composition compared to strains found in caries-free (CF) children. Unique genetic loci that are specific to S-ECC strains will be identified and characterized. Some of these genetic loci will prove informative as biomarkers for health or disease. Using the power of multiple test analyses derived artificial intelligence programming; biomarkers will be used as the basis for a modeling algorithm capable of classifying strains of S. mutans in one of two categories (S-ECC or CF). The second aim of this study is to survey strains of S. mutans associated with either S-ECC or CF status for known or suspected genetic elements thought to influence the pathogenesis or virulence of S. mutans. The third aim is to survey a larger, racial/ethnical diverse population of S-ECC or CF children for the presence or absence of the biomarkers identified in the first two aims. It is anticipated that this research will yield genetic markers for disease capable of providing clinicians with a diagnostic test that can assess risk for developing S-ECC. 7.

描述（由申请方提供）：预期拟定的研究将产生关于变形链球菌中存在特定遗传决定因素的重要新信息，变形链球菌是一种与影响幼儿的严重龋齿（称为严重幼儿龋齿或S-ECC）密切相关的微生物。这些信息的长期利益应该引导研究人员设计针对牙科的诊断和预防策略，尽管更好地了解其微生物原因。利用抑制性差减DNA杂交的强大技术，第一个具体的目标是测试假设，S。与无龋（CF）儿童中发现的菌株相比，S-ECC儿童中发现的变异株的遗传组成不同。将对S-ECC菌株特异性的独特遗传基因座进行鉴定和表征。这些基因位点中的一些将被证明是健康或疾病的生物标志物。使用多个测试分析的力量衍生人工智能编程;生物标志物将被用作能够对S.两种类型之一的变异（S-ECC或CF）。本研究的第二个目的是调查S.与S-ECC或CF状态相关的已知或疑似遗传元件被认为影响S.变异体第三个目的是调查更大的，种族/民族多样化的S-ECC或CF儿童人群中是否存在前两个目的中确定的生物标志物。预计这项研究将产生疾病的遗传标记，能够为临床医生提供诊断测试，可以评估发展S-ECC的风险。7.

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Streptococcus mutans and Streptococcus sobrinus colonization and caries experience in 3- and 5-year-old Thai children.

在3岁和5岁的泰国儿童中，链球菌和Sobrinus链球菌定植和龋齿经历。

DOI：
10.1007/s00784-015-1437-0
发表时间：
2015-11
期刊：
Clinical oral investigations
影响因子：
3.4
作者：
Saraithong P;Pattanaporn K;Chen Z;Khongkhunthian S;Laohapensang P;Chhun N;Pattanaporn W;Gaw HY;Li Y
通讯作者：
Li Y

Streptococcus mutans and Streptococcus sanguinis colonization correlated with caries experience in children.

DOI：
10.1159/000159608
发表时间：
2008
期刊：
Caries research
影响因子：
4.2
作者：
Ge Y;Caufield PW;Fisch GS;Li Y
通讯作者：
Li Y

Comparative genomics of oral isolates of Streptococcus mutans by in silico genome subtraction does not reveal accessory DNA associated with severe early childhood caries.

通过计算机基因组消减对变形链球菌口腔分离株进行的比较基因组学并未揭示与严重儿童早期龋齿相关的辅助 DNA。

DOI：
10.1016/j.meegid.2013.11.003
发表时间：
2014
期刊：
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
影响因子：
0
作者：
Argimón,Silvia;Konganti,Kranti;Chen,Hao;Alekseyenko,AlexanderV;Brown,Stuart;Caufield,PageW
通讯作者：
Caufield,PageW

Phylogenetic analysis of glucosyltransferases and implications for the coevolution of mutans streptococci with their mammalian hosts.

葡萄糖基转移酶的系统发育分析及其对肌肉链球菌与哺乳动物宿主的共同进化的影响。