Digital Microfluidics SAWN

数字微流控SAWN

• 批准号: 8834920
• 负责人: ERIK J NILSSON
• 金额: $ 14.68万
• 依托单位: DEURION, LLC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-12-12 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8834920
• 关键词: Acoustics; Adhesions; Affinity; Automation; Biomedical Research; Blood capillaries; Caseins; Chemistry; Clinical; Complex; Coupled; Data; Detection; Development; Devices; Diagnostic; Digestion; Electrodes; Frequencies; Knowledge; Laboratories; Lead; Liquid substance; Marketing; Mass Spectrum Analysis; Metals; Microfluidics; Modification; Noise; Oxidation-Reduction; Pattern; Peptides; Phase; Phosphopeptides; Preparation; Process; Protein Denaturation; Proteins; Proteolysis; Proteomics; Reagent; Research; Risk; Sampling; Signal Transduction; Structure; Surface; Technology; Time; Training; Transducers; Work; base; capillary; cost effective; digital; evaporation; flexibility; improved; ion source; ionization technique; magnetic beads; micro-total analysis system; nanolitre; operation; public health relevance; reconstitution; tool; voltage

 DESCRIPTION (provided by applicant): This proposal is for biomedical applications of DMF-SAWN (Digital Microfluidics - Surface Acoustic Wave Nebulization), a highly flexible lab-on-a-chip device that can be directly coupled to mass spectrometry (MS). DMF circumvents the need for time intensive labor to produce traditional micro valve networks essential in fine manipulation of fluids. Dead volume-free preparatory steps are easily integrated without the need for enclosed micro channels, which over time can clog from debris or surface adhesion build- up. In DMF, arrays of metal electrodes are patterned on a dielectric surface and then treated with a hydrophobic layer. DMF can manipulate a greater range of volumes (nL-mL) than micro channel-based fluidics and offers streamlined multi-step sample processing such as, but not limited to extraction, purification, reconstitution, and digestion. Because the mechanism of DMF relies on a dielectric substrate, this offers a unique opportunity to couple to MS using a recently developed planar ionization technique called surface acoustic wave nebulization (SAWN). The advantages SAWN over ESI: (1) like DMF it is planar allowing the two to be readily coupled without expert knowledge of capillary based fluidics, (2) absence of the high DC voltage used in ESI SAWN produces higher signal / noise due to the elimination of redox chemistry products, (3) droplet size is inversely proportional to applied frequency allowing droplet size to be tailore to suit the need, and (4) it may be operated in a discontinuous mode allowing nanoliter-volume samples to be analyzed. Completion of this proposal will results in an MS ion source product incorporating a lab-on-a-chip. The target market is mass spectrometry, primarily for biomedical research and clinical diagnostics.

 描述（由申请人提供）：本提案适用于DMF-SAWN（数字微流体-表面声波雾化）的生物医学应用，DMF-SAWN是一种高度灵活的芯片实验室设备，可直接与质谱仪（MS）耦合。DMF避免了需要时间密集的劳动力来生产传统的微阀网络在精细操作中必不可少的 液体。无死体积的制备步骤容易集成，而不需要封闭的微通道，其随着时间的推移可能因碎片或表面粘附积聚而堵塞。在DMF中，金属电极阵列在电介质表面上形成图案，然后用疏水层处理。与基于微通道的流体技术相比，DMF可以处理更大范围的体积（nL-mL），并提供简化的多步样品处理，例如但不限于提取、纯化、重构和消化。因为DMF的机制依赖于介电衬底，所以这提供了使用最近的电介质耦合到MS的独特机会。 开发了称为表面声波雾化（SAWN）的平面电离技术。SAWN相对于ESI的优势：（1）类似于DMF，它是平面的，允许两者容易地耦合，而无需基于毛细管的射流的专业知识，（2）由于消除了氧化还原化学产物，在ESI SAWN中使用的高DC电压的缺失产生更高的信号/噪声，（3）液滴尺寸与施加的频率成反比，允许液滴尺寸被定制以适合需要，以及（4）它可以以允许分析纳升体积样品的不连续模式操作。该提案的完成将导致MS离子源产品包含芯片实验室。目标市场是质谱仪，主要用于生物医学研究和临床诊断。