Malaria screening in resource-poor settings using a simple, power-free, cell phone-friendly device

使用简单、无电源、适合手机使用的设备在资源匮乏的环境中进行疟疾筛查

• 批准号: 8925940
• 负责人: IONITA Calin GHIRAN
• 金额: $ 19.93万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-10 至 2016-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8925940
• 关键词: Acute; Adult; African; Algorithms; Anemia; Antibodies; Area; Artemisinins; Back; Beds; Bite; Blood; Blood Cells; Blood Platelets; Blood capillaries; Blood specimen; Buffers; Burkitt Lymphoma; Cell Separation; Cell physiology; Cellular Phone; Cessation of life; Child; Child Mortality; Chronic; Code; Collaborations; Comorbidity; Complex; Complication; Culicidae; Custom; Data; Detection; Devices; Diagnosis; Diagnostic; Diagnostic Procedure; Diagnostic tests; Disease; Drops; Drug resistance; Emergency treatment; Equipment; Erythrocytes; Fatal Outcome; Fever; Geometry; Glass; Goals; Gold; Health; Health Services; Health system; Heating; Hematology; Hemoglobin; Hospitals; Hour; Human Resources; Image; Image Analysis; Immune; Immunity; Individual; Infection; Insecticides; Ions; Laboratories; Lead; Letters; Leukocytes; Life; Location; Magnetism; Malaria; Malignant Neoplasms; Malnutrition; Measures; Methods; Microscope; Microscopic; Microscopy; Nigeria; Outcome; Parasites; Pathology; Patients; Pharmaceutical Preparations; Plasmodium; Plasmodium falciparum; Plastics; Pregnancy; Prevalence; Process; Property; Provider; Public Health; Reaction; Reagent; Recurrence; Resources; Risk; Role; Sensitivity and Specificity; Shapes; Sickle Cell Anemia; Solutions; Splenomegaly; Staining method; Stains; Structure; Symptoms; System; Technology; Telephone; Temperature; Testing; Time; Training; Universities; Work; Writing; artemisinine; base; capillary; cell type; cloud based; cost; drug testing; early childhood; efficacy testing; feeding; hemozoin; image processing; improved; innovation; interest; lens; light microscopy; light weight; mortality; novel; pathogen; prevent; programs; prototype; research clinical testing; screening; stability testing; tool; vector mosquito

DESCRIPTION (provided by applicant): Malaria is a life-threatening disease caused by one the four species of Plasmodium parasites that are transmitted to people through the bites of infected mosquitoes. In 2012 alone, malaria caused over 625 000 deaths, mostly among African children. Due to its high child mortality and prevalence in Sub-Saharan regions, in 2000 a new program was launched in Nigeria, the Roll Back Malaria (RBM), aiming at reducing the burden of malaria by using presumptive treatment (PT) based, in many cases, only on fever as single sign of malaria infection. This approach, necessary due to limited laboratory support leading to long waiting times for laboratory diagnostic and consequently increased risks associated with delayed anti-malaria treatments, is often the only available option for local health service providers. Although studies have shown a significant decrease in malaria burden since the beginning of the RBM program in areas where the PT approach is employed, continuation of this practice already led to over-diagnosis of the malaria, under-diagnosis of other illness, increased drug wastage, increase adverse anti-malaria drug reactions, increased parasite-drug- resistance, and unnecessary financial burden to local health systems. We propose to develop an antibody- free diagnostic screening device that is based on the principles of magnetic levitation, allowing for separation of infected red blood cells from un-infected red blood cells. Our screening device will be light-weight, disposable, and cheap to manufacture with an indefinite shelf life at room temperature. The prototype system requires less than a drop of fingerprick blood (<1 �L) and a small volume of RBC-friendly buffer containing paramagnetic ions. Binary diagnostic results is obtained within a few minutes solely by using a set of permanent magnets immobilized in a plastic structure surrounding a glass/plastic capillary containing the blood. Results are recorded and stored using a standard camera phone. No additional imaging equipment, staining reagents, or consumables are required.

描述（由适用提供）：疟疾是一种威胁生命的疾病，是由四种疟原虫寄生虫通过感染蚊子传播给人们的四种。仅在2012年，疟疾就导致超过62.5万人死亡，主要是在非洲儿童中。由于其在撒哈拉以南地区的儿童死亡率很高和患病率，2000年在尼日利亚启动了一项新计划，后背疟疾（RBM）旨在通过许多情况下以一种基于自然的治疗（PT）来减少疟疾的燃烧（在许多情况下，仅用于发烧作为单一疟疾症状的迹象。由于实验室的支持有限，因此需要长期等待实验室诊断，因此这种方法通常是当地卫生服务提供者的唯一可用选择。尽管研究表明，自RBM计划开始以来，在采用PT方法的领域开始疟疾，这种做法的延续已经导致疟疾过度诊断，其他疾病的诊断不足，药物浪费增加，不良反恶性药物反应增加，寄生虫 - 寄生虫 - 寄生虫 - 贫民窟抗药性增加，并增加了本地卫生系统。我们建议开发一种基于磁悬浮原理的无抗体诊断筛查装置，从而使感染的红细胞与未感染的红细胞分离。我们的筛选设备将在室温下使用无限期的保质期生产轻巧，一次性和便宜。原型系统需要小于一滴指纹血液（<1-l）和少量含有顺磁离子的RBC友好缓冲液。仅使用一组固定在包含血液的玻璃/塑料毛细管周围的塑料结构中的永久磁体，仅在几分钟内获得二进制诊断结果。使用标准的照相手机记录并存储结果。不需要其他成像设备，染色试剂或消耗品。

项目成果

Sickle cell detection using a smartphone.

• DOI: 10.1038/srep15022
• 发表时间: 2015-10-22
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Knowlton SM;Sencan I;Aytar Y;Khoory J;Heeney MM;Ghiran IC;Tasoglu S
• 通讯作者: Tasoglu S

Smart-Phone Based Magnetic Levitation for Measuring Densities.

• DOI: 10.1371/journal.pone.0134400
• 发表时间: 2015
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Knowlton S;Yu CH;Jain N;Ghiran IC;Tasoglu S
• 通讯作者: Tasoglu S

Detection of membrane-bound and soluble antigens by magnetic levitation.

• DOI: 10.1039/c7lc00402h
• 发表时间: 2017-10-11
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Andersen MS;Howard E;Lu S;Richard M;Gregory M;Ogembo G;Mazor O;Gorelik P;Shapiro NI;Sharda AV;Ghiran I
• 通讯作者: Ghiran I