A new device to improve screening of corneal transplant tissue

詹姆斯·马鲁斯眼库内皮细胞计数器 摘要 获取供移植用的角膜组织是一项重要的社会服务。 主要由资金不足的眼科银行提供。组织不仅要被收获，而且必须进行筛选，以便 可能使组织不适合移植或对健康构成风险的无数问题 纸巾接受者。供体组织可能会因形态问题而被拒绝，包括先前的屈光不正 手术(LASIK)或低内皮细胞计数。接受过手术的供体组织 保留上皮瓣，如LASIK，不能用于移植，因为皮瓣会分离 在缝合过程中。低内皮细胞计数也是供体组织低排斥反应的基础 内皮细胞计数是严重角膜水肿的先兆。 目前，眼库在组织收获后对捐赠者组织进行筛查以发现这些问题 要么作为一个整体，要么作为一个角膜巩膜边缘。组织被送回眼库实验室 进行各种测试，包括裂隙灯检查，以确定组织有效性和 镜面显微镜测定内皮细胞计数。 在之前的一个资助项目中，VOL开发了一种便携式眼部扫描仪设备，用于筛选组织 在采集组织之前，在实地进行屈光手术。此设备不仅会生成一个 确定屈光手术存在与否的最终指标，但也降低了成本 如果组织被认为是不可用的，与组织采集相关。仅当前设备 扫描角膜前地形图以了解屈光诱导的形状变化和通过该区域的组织 在释放用于移植之前，检测仍然需要进一步检查内皮细胞计数。 因此，目前的提案寻求增加通过以下方式确定内皮细胞计数的能力 对现有设备进行改造，以生产一种既能扫描前角膜又能扫描角膜的新设备 检查角膜地形图是否有屈光手术的迹象，并进行内皮细胞计数。这样的装置 将允许在组织采集之前对这两个标准进行筛查，从而节省了显著的成本 还有时间。

Marous, James R. An Eye Bank Endothelial Cell Counter Abstract Procurement of donor corneal tissue for transplant use is an important social service undertaken by largely underfunded eye banks. Tissue is not only harvested, but must be screened for a myriad of issues that may make the tissue unsuitable for transplant or pose a health risk to the tissue recipient. Donor tissue may be rejected for morphological issues including prior refractive surgeries (LASIK) or low endothelial cell count. Donor tissue that has undergone a procedure leaving an epithelial flap, such as LASIK, cannot be use for transplant as the flap will separate during suturing. Low endothelial cell count is also a basis for rejection of donor tissue as low endothelial cell count is a precursor to substantial corneal edema. Currently, eye banks screen donor tissue for these issues after the tissue has been harvested either as a whole globe or a corneal-scleral rim. Tissue is returned to the eye bank laboratory and a variety of tests are performed including slit lamp examinations to determine tissue efficacy and specular microscopy to determine endothelial cell count. In a prior funded project, VOL has developed a portable eye scanner device to screen tissue for prior refractive surgeries in the field, prior to tissue harvesting. This device not only generates a definitive metric for the determination of refractive surgery presence, but also reduces the cost associated with tissue harvesting should the tissue be deemed unusable. The current device only scans anterior corneal topography for refractive induced shape changes and tissue that passes this test still requires further examination for endothelial cell count before release for transplant. The current proposal therefore seeks to add the ability to determine endothelial cell count by modifying the existing device to produce a new device that will both scan anterior corneal topography for signs of refractive surgery and perform an endothelial cell count. Such a device will allow screening of both criteria before tissue harvesting thereby saving both significant cost and time.