IGF::OT::IGF CBCSLC TASK ORDER FOR THE NCI CHEMICAL BIOLOGY CONSORTIUM, TO# 1 12 MONTH TASK ORDER BASE

NCI 化学生物联盟的 IGF::OT::IGF CBCSLC 任务单，至

• 批准号: 9154514
• 负责人: CHARLES JOHNSON
• 金额: $ 20.47万
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2020-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9154514
• 关键词: Biology; Businesses; Characteristics; Charge; Chemical Structure; Chemicals; Cherry - dietary; China; Collection; Contractor; Contracts; Custom; Data; Databases; Descriptor; Dimethyl Sulfoxide; Diversity Library; Documentation; Dry Ice; Ensure; Equipment; Equipment and supply inventories; Film; Government; High Pressure Liquid Chromatography; India; Individual; Informatics; Institutes; International; Ions; Libraries; Life; Maintenance; Methods; Molecular; Monitor; Powder dose form; Property; Quality Control; Recording of previous events; Regulation; Research Infrastructure; Research Personnel; Sampling; Shipping; Ships; Solid; Solutions; Solvents; Specific qualifier value; Structure; Tube; Update; Vial device; Work; base; cost; cost effective; design; falls; flexibility; high throughput analysis; instrument; liquid chromatography mass spectrometry; meetings; member; planetary Atmosphere; quality assurance; sample collection; screening; solid solution

The listed tasks will be performed on an on-going basis throughout the contract life. a. Storage- Storage of the approximately 135,000 compound CBCSLC library in a suitable facility under conditions that ensure optimal stability and purity, along with background tasks related to the maintenance of associated infrastructure. The library collection is maintained as a mix of: 1) 4 dram vialed neat solid/films, 2) working solution stores in Matrix tubes and 3) 384-well microtiter plates. The Contractor shall have the capability to store all samples long-term at -78, -20, and 0°C under inert atmosphere as appropriate for maintaining quality in a cost effective manner. The standard storage conditions are -20°C under an inert atmosphere employing DMSO as a solution solvent. There shall be sufficient storage space and flexibility to accommodate changes in the mix of storage types. The Contractor shall provide all the necessary expertise, equipment, and facilities to store, maintain, and track the inventory. Storage and handling shall comply with all applicable local, state and Federal regulations. – ANY AND ALL COMPOUNDS SUPPLIED OR PURCHASED UNDER THIS CONTRACT, STORED AS SOLIDS, SOLUTIONS OR PLATED, SHALL REMAIN THE PROPERTY OF THE GOVERNMENT THROUGHOUT THE LIFE OF THE CONTRACT. THE CONTRACTOR SHALL TRANSFER THESE COMPOUNDS TO THE FOLLOW-ON CONTRACT SHOULD ONE BE ISSUED OR TRANSFER THE COMPOUNDS TO THE APPROPRIATE FACILITY AS DIRECTED BY THE CONTRACTING OFFICER OR HIS REPRESENTATIVE. See section K of this SOW - b. Quality Control – The Contractor shall perform rapid, low throughput analyses of chemical purity (e.g., LC/MS, HPLC, and/or NMR) as requested by the COR. It is anticipated that this will involve 50-250 samples annually. The Contractor shall be required to have an on-going Quality Assurance plan for instruments and other equipment. The QC requirement (purity and identity) shall include but is not limited to ability to carry out high throughput analysis by GC-MS or LC-MS and interpret results as passing or failing to meet QC criteria by showing specified purity (as specified by the COR). The primary method of analysis shall be LC-MS and/or GC-MS. When these methods are not suitable the Contractor shall have the capability to routinely perform purity analysis by 1H-NMR. It is anticipated that <1% of samples will require 1H-NMR analysis. Structure not confirmed by lack of observed molecular ion by MS or NMR spectra inconsistent with structure is deemed as failing. c. Distribution – The Contractor shall have the capability to ship plated sets (e.g. diversity library, focused libraries and single-use sets) to the CBC screening centers and external investigators, as requested by the COR. The Contractor shall have capability to ship on a weekly basis using “next business day” for domestic shipments. The Contractor shall also have the capability to ship internationally including regions in India and China. It is estimated that international shipments will be minimal. It is anticipated that 10-15 institutes will comprise the CBC including 5-8 screening centers. Compound shipments shall be made in such a way as to ensure the integrity of the contents (e.g. packed in dry ice) and shall comply with all applicable federal, state, and local regulations and international shipping and custom regulations. It is anticipated that 15-20 individual shipments will be made annually that include existing libraries and cherry-picked sets. The direct shipping costs will be charged to the recipient through their Fedex number while the cost associated with packaging will be the responsibility of the Contractor. d. Inventory Monitoring and Repurchase- The Contractor shall monitor and maintain adequate compound inventory, both solution and dry store, to ensure that sufficient threshold quantities, as specified by the COR, of all compounds are available to support screening needs. As compounds fall below threshold inventory (e.g. 5 mg), either by depletion or degradation, the Contractor shall undertake all activities necessary to purchase sufficient quantities of the compound to replenish the inventory upon approval by the COR. It is anticipated that no more than 0.5% of the sample collection will need to be replenished annually. It is estimated that this would be ~300 compounds over the life of the contract. e. CBC Synthetic Compounds – The Contractor shall also have the capability to manage CBC member generated dry powder synthetic samples. It is anticipated that the number of compounds will be minimal over the life of the contract (i.e. <50). Management of samples will involve receipt, registration, inventory, storage, analysis and distribution activities. f. Disposal – The Contractor shall comply with all local, state and Federal regulations in the disposal of chemical samples, personal protective equipment, solvents and chemically contaminated vials, tubes and plates. g. Informatics and Documentation - The Contractor shall maintain and update the CBCSLC electronic database containing individual chemical structures, descriptors, associated physiochemical data along with distribution and inventory information. The Contractor’s database shall be functionally compatible with the internal Oracle-based NCI/CBC database. The Contractor shall be responsible for regularly synchronizing the CBC-related information between the databases. In addition, the Contactor shall produce archival documents that describe the design history, physiochemical characteristics and content of new libraries added to the collection over the life of the contract.

所列任务将在整个合同期内持续执行。 a. 储存-在确保最佳稳定性和纯度的条件下，将约135，000个化合物CBCSLC文库储存在合适的设施中，沿着与相关基础设施维护相关的后台任务。 文库收集物保持为以下物质的混合物：1）4个小瓶中的纯固体/膜，2）储存在Matrix管中的工作溶液和3）384孔微量滴定板。 承包商应能够在惰性气氛下将所有样品长期储存在-78、-20和0°C下，以保持质量，并具有成本效益。 标准储存条件是在惰性气氛下在-20 ° C下使用DMSO作为溶液溶剂。 应有足够的储存空间和灵活性，以适应储存类型组合的变化。 承包商应提供所有必要的专业知识、设备和设施，以储存、维护和跟踪库存。 储存和搬运应符合所有适用的地方、州和联邦法规。 - 根据本合同提供或购买的任何及所有化合物，以固体、溶液或电镀形式储存，在整个合同有效期内，应归政府所有。 承包商应将这些化合物转移到后续合同中，如果其中一种化合物被签发或转移到适当的工厂，由招标官员或其代表指示。 参见本工作说明书的第K节- B. 质量控制-承包商应进行快速、低通量的化学纯度分析（例如，LC/MS、HPLC和/或NMR）。 预计每年将采集50-250份样本。 应要求承包商制定仪器和其他设备的持续质量保证计划。 QC要求（纯度和鉴别）应包括但不限于通过GC-MS或LC-MS进行高通量分析的能力，并通过显示规定的纯度（如COR所规定）将结果解释为通过或不符合QC标准。主要分析方法应为LC-MS和/或GC-MS。当这些方法不适用时，承包商应能够通过1H-NMR进行常规纯度分析。 预计<1%的样品将需要1H-NMR分析。 通过MS或NMR光谱未观察到与结构不一致的分子离子而未确认结构，视为不合格。 C. 分发-承包商应能够按照COR的要求，将平板套件（例如多样性文库、重点文库和一次性套件）运送至CBC筛查中心和外部研究者。 承包商应能够每周使用“下一个工作日”进行国内装运。 承包商还应具备国际运输能力，包括印度和中国地区。 据估计，国际货运量将是最低限度的。 预计CBC将由10-15个机构组成，其中包括5-8个筛查中心。 化合物的运输应确保内容物的完整性（例如，用干冰包装），并应符合所有适用的联邦、州和地方法规以及国际运输和海关法规。 预计每年将进行15-20次单独装运，其中包括现有的图书馆和精选集。 直接运输费用将通过其联邦快递号码向收件人收取，而与包装相关的费用将由承包商负责。 D. 库存监控和回购-承包商应监控并保持充足的化合物库存，包括溶液和干燥储存，以确保COR规定的所有化合物的足够阈值数量可用于支持筛选需求。 当化合物因消耗或降解而低于阈值库存（例如5 mg）时，承包商应采取一切必要措施，购买足够数量的化合物，以在COR批准后补充库存。 预计每年需要补充的样本量不超过0.5%。 据估计，这将是约300化合物的合同寿命。 e. CBC合成化合物-承包商还应具有管理CBC成员生成的干粉合成样品的能力。 预计在合同有效期内，化合物的数量将是最少的（即<50）。 样品管理将涉及接收、登记、库存、储存、分析和分发活动。 F. 处置-承包商应遵守所有地方、州和联邦法规，处置化学样品、个人防护装备、溶剂和化学污染的小瓶、管子和平板。 G. 信息和文件-承包商应维护和更新CBCSLC电子数据库，其中包含单个化学结构、描述符、相关理化数据沿着分布和库存信息。 承包商的数据库应在功能上与基于Oracle的内部NCI/CBC数据库兼容。 承包商应负责定期同步数据库之间的CBC相关信息。 此外，承包商应编制档案文件，描述在合同有效期内增加的新图书馆的设计历史、理化特性和内容。