Animal Physiology Core

动物生理学核心(APC)使用复杂的研究方法来帮助爱因斯坦-芒特 西奈糖尿病研究中心(ES-DRC)研究人员在体内评估葡萄糖和脂肪酸 小鼠和大鼠的代谢、胰岛素敏感性和能量稳态。通过与 作为ES-DRC的其他核心，APC使调查人员能够彻底表征定义的 药物、饮食、环境和遗传对糖脂稳态、胰岛素的影响 行动和新陈代谢。核心执行脂肪分布的研究，并促进核磁共振波谱， 代谢研究糖尿病实验动物模型的fmri和microPET分析。这个 CORE还为研究实验室和几种心脏功能提供专门的啮齿动物手术 与糖尿病并发症相关的评估。为了实现这些目标，动物生理学核心将：1) 建议研究人员设计与控制葡萄糖稳态和代谢相关的研究 胰岛素在啮齿动物中的作用；2)向研究人员提供全身和 组织特异性葡萄糖敏感性和胰岛素作用，包括但不限于胰岛素、胰腺和 高血糖钳夹研究；3)为胰岛素敏感性、能量研究提供专门的手术模型 平衡、葡萄糖和脂肪酸代谢；4)向学生、博士后研究员、 研究人员和技术人员执行必要的外科和生理技术以评估 控制血糖稳态和胰岛素的作用；5)提供全身碳水化合物/脂肪酸的分析 氧化、能量消耗、摄食行为和利用特殊代谢的运动活动(间接 量热法)和行为鼠笼；6)提供自发或预定的影响的评估 对葡萄糖动态平衡和新陈代谢的锻炼；7)向专门研究人员提供 用显微CT测量啮齿动物脂肪组织分布及肝脏和肝脏糖原含量 肌肉、肝内脂肪和心肌细胞内脂肪使用核磁共振；8)向专门研究人员提供 通过功能磁共振成像(FMRI)和microPET扫描测量大脑能量和葡萄糖利用； 协调这些努力与爱因斯坦和西奈山的其他ES-DRC和机构核心设施。全 这些服务适用于糖尿病研究的新手以及从事糖尿病研究的研究人员 与糖尿病相关的项目，可以通过使用本组织的专门知识和设施来丰富和扩展 核心。

The Animal Physiology Core (APC) employs sophisticated research methodologies to assist Einstein-Mount Sinai Diabetes Research Center (ES-DRC) investigators in the in vivo assessment of glucose and fatty acid metabolism, insulin sensitivity and energy homeostasis in mice and rats. Through collaborative efforts with the other Cores of the ES-DRC, the APC enables investigators to thoroughly characterize the effects of defined pharmacological, dietary, environmental and genetic alterations on glucose and lipid homeostasis, insulin action, and metabolism. The Core performs studies of adiposity distribution and facilitates NMR spectroscopy, fMRI and microPET analysis of experimental animal models of diabetes undergoing metabolic studies. The Core also provides specialized rodent surgeries for investigator laboratories and several cardiac functional assessments related to diabetic complications. To accomplish these goals, the Animal Physiology Core will: 1) advise investigators in the design of metabolic studies relevant to the control of glucose homeostasis and insulin action in rodents; 2) make available to investigators specialized measurements of whole body and tissue-specific glucose sensitivity and insulin action including, but not limited to insulin, pancreatic and hyperglycemic clamp studies; 3) provide specialized surgical models for the study of insulin sensitivity, energy balance, and glucose and fatty acid metabolism; 4) offer instruction to students, postdoctoral fellows, investigators and technical staff in performing surgical and physiological techniques necessary to evaluate the controls of glucose homeostasis and insulin action; 5) provide analysis of whole body carbohydrate/fatty acid oxidation, energy expenditure, feeding behavior, and locomotor activity using specialized metabolic (indirect calorimetry) and behavioral rodent cages; 6) provide assessment of the effects of spontaneous or scheduled exercise on glucose homeostasis and metabolism; 7) make available to investigators specialized measurements of rodent adipose tissue distribution using microCT and measurements of glycogen in liver and muscle, intrahepatic lipids and intramyocellular lipids using NMR; 8) make available to investigators specialized measurements of brain energy and glucose utilization by functional MRI (fMRI) and microPET scanning; and 9) coordinate these efforts with other ES-DRC and Institutional Core facilities at Einstein and Mount Sinai. All these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this Core.