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Tissue Core

组织核心

基本信息

批准号：
8935036
负责人：
Mark J Ferris
金额：
$ 12.94万
依托单位：
WAKE FOREST UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8935036
关键词：
Acute Animals Behavioral Binding Biochemical Biopsy Blinded Blood Brain Cerebrospinal Fluid Chronic Cocaine Data Dopamine D2 Receptor Double-Blind Method Drug Combinations Drug or chemical Tissue Distribution Ensure Exposure to Future GTP-Binding Proteins Gene Expression Generations Genes Goals Image In Vitro Infusion procedures International Lentivirus Vector Mediating Neurobiology Pathway interactions Peripheral Pharmaceutical Preparations Plasma Preparation Procedures Pump Rattus Recording of previous events Research Personnel Rodent Sampling Second Messenger Systems Self Administration Tissue Banking Tissue Banks Tissue Sample Tissues Venous Viral addiction animal tissue brain tissue in vivo information organization nonhuman primate postsynaptic presynaptic radioligand receptor receptor function research study second messenger small hairpin RNA subcutaneous trafficking

项目摘要

Center for the Neurobiology of Addiction Treatment Animal and Tissue Core Summary Dr. Mark Ferris, Core Director; Dr. Paul Czoty, co-investigator The overall objective of the Animal and Tissue Core is to generate, track and distribute tissue from experimental animals to multiple in vivo and in vitro experiments. The Core will centralize and standardize the generation, storage and transfer of brain tissue from rodents to Projects 2 and 3. These studies include self- administration, viral mediated gene knockdown of dopaminergic D2 receptors and acute/chronic treatment with putative medications identified in Project 1. By taking responsibility for the generation of these animals for studies in Projects 2 and 3, the Core will ensure that all subjects will be treated and euthanized in a standardized manner. Brain and peripheral tissue will be organized and stored by the Core, which will ensure accurate recording of the subjects' behavioral and pharmacological histories. The Core will distribute this tissue under blinded conditions to investigators in Projects 2 and 3 for imaging and biochemical studies. Careful preparation and handling of these samples and meticulous organization of information are required to ensure that reliable conclusions can be obtained from experiments using these tissue samples, which makes the Animal and Tissue Core an essential component of the Center. The specific goals are: 1. To generate groups of rats with viral mediated gene knockdown targeted against D2 receptors for Projects 2 and 3. 2. To generate groups of rats with a history of cocaine self-administration (with and without medication treatment and viral mediated gene knockdown) for Projects 2 and 3. 3. To provide a central locus for detailed record-keeping for all tissues stored within the Core and to provide timely distribution of this tissue under double-blinded conditions to Projects 2 and 3. 4. To collect and bank rodent brains that will be generated by cocaine self-administration studies in Project 1 for biochemical analyses of putative treatment drugs and other potential future uses. 5. To collect and bank nonhuman primate blood and cerebrospinal fluid (CSF) for analysis of metabolites of putative treatment drugs and other potential future uses, and bank nonhuman primate brain samples as they become available after studies in Project 1 are completed. 6. To maintain the tissue bank and periodically biopsy tissue to ensure tissue integrity.

成瘾治疗神经生物学中心