Can direct pathway stimulation cause addiction-like changes in behavior?

直接通路刺激能否导致类似成瘾的行为变化？

• 批准号: 8939740
• 负责人: Alexxai V Kravitz
• 金额: $ 58.27万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8939740
• 关键词: Behavior; Behavioral; Biological Assay; Canned Foods; Corpus striatum structure; Disease; Drug Addiction; Environment; Food; Future; Learning; Motivation; Mus; Neurons; Pathway interactions; Phenotype; Process; Psychological reinforcement; Stimulus; Taste Perception; Testing; Time; Training; Work; addiction; cell type; experience; food addiction; novel; optogenetics; reinforced behavior; therapeutic target; willingness

In this project, we will train mice to self-stimulate their direct pathway medium spiny neurons (dMSNs) using optogenetics. In prior work, we have found that mice find this behavior highly reinforcing. However, prior work gave mice at most 90 minutes of total training time. We plan to expand this by a factor of 100, giving the mice much more experience with this highly reinforcing behavior. We will examine behavioral changes in these mice, including assaying their motivation to receive stimulation, willingness to work for stimulation, and willingness to endure negative consequences to receive stimulation.

在这个项目中，我们将使用光遗传学训练小鼠自我刺激它们的直接通路中棘神经元（dMSN）。 在之前的工作中，我们发现小鼠发现这种行为具有高度的强化作用。 然而，先前的工作给小鼠最多90分钟的总训练时间。 我们计划将其扩大到100倍，让小鼠对这种高度强化的行为有更多的经验。 我们将研究这些小鼠的行为变化，包括分析它们接受刺激的动机，为刺激而工作的意愿，以及接受刺激而忍受负面后果的意愿。