mRNA Degradation in Borrelia burgdorferi

伯氏疏螺旋体中 mRNA 的降解

• 批准号: 8870515
• 负责人: PAULA J SCHLAX
• 金额: $ 34.58万
• 依托单位: BATES COLLEGE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8870515
• 关键词: Accounting; Antibiotics; Arthropods; Bacteria; Binding; Biochemical; Borrelia burgdorferi; Centers for Disease Control and Prevention (U.S.); Characteristics; Cleaved cell; Consensus; Consensus Sequence; Data; Degradation Pathway; Development; Diagnostic; Disease; Elements; Gene Expression; Gene Expression Regulation; Genes; Genetic study; Human; Individual; Ixodes; Kinetics; Knowledge; Life Cycle Stages; Lyme Disease; Marketing; Membrane Proteins; Messenger RNA; Microbiology; Molecular; Nature; Order Spirochaetales; Organism; OspA protein; Pathogenesis; Pathway interactions; Play; Prevention; RNA; RNA Degradation; RNA Sequences; RNA-Binding Proteins; Reporting; Research Personnel; Ribonuclease III; Ribonucleases; Rodent; Role; Site; Staging; Structure; Ticks; Transcript; Translational Regulation; United States; Untranslated RNA; Vaccines; Vector-transmitted infectious disease; Virulence Factors; base; design; enzootic; feeding; improved; infected vector rodent; insight; mRNA Decay; mRNA Transcript Degradation; microorganism; novel; pathogen; protein B; public health relevance; research study; transcriptome sequencing; transmission process; treatment strategy; vector

 DESCRIPTION (provided by applicant): Borrelia burgdorferi, the causative agent of Lyme disease, requires shifts in gene expression to undergo its natural enzootic cycle between tick and vertebrate hosts. mRNA degradation is an important mechanism for gene regulation, but is not yet characterized in B. burgdorferi. We propose to characterize the roles of specific ribonucleases in mRNA turnover by comparing the decay rates and steady state abundances of transcripts in wild-type and ribonuclease deficient strains of B. burgdorferi. We will determine the consensus sequences and structures of mRNA recognized and cleaved by ribonucleases. In order to gain insight into the relationship between translational regulation and mRNA decay for genes important in the enzootic cycle, we will examine the rates of mRNA decay of transcripts in the presence and absence of their translational regulators. The data from the proposed experiments will provide researchers with , will be the first to provide insight into the roles of individual ribonucleases, characteristics of ribonuclease cleavage sites, and relationships between translational regulation and mRNA decay for selected genes in this important pathogen. Lyme disease infects approximately 300,000 U.S. citizens each year. A better understanding of the molecular mechanisms of gene regulation, including those involving mRNA decay, that are used by this pathogen to survive the transition between vector and host is essential for the design of improved diagnostic and treatment strategies.

 描述（由适用提供）：莱姆病的致病药物Borrelia Burgdorferi需要基因表达的转变，以在tick虫和脊椎动物宿主之间经历其自然循环。 mRNA降解是基因调节的重要机制，但尚未在B. burgdorferi中表征。我们建议通过比较野生型和核糖核酸酶中的转录本的衰减率和稳态丰富性来表征特异性色带菌群在mRNA周转中的作用。我们将确定通过核糖珠识别和清除的mRNA的共识序列和结构。为了深入了解在Enzootic循环中重要的基因的转化调节与mRNA衰减之间的关系，我们将在存在和不存在翻译调节剂的情况下检查转录物的mRNA衰减速率。提出的实验的数据将为研究人员提供，将是第一个洞悉单个色带裂解位点的作用，以及在这种重要病原体中所选基因的翻译调节与mRNA衰变之间的关系。莱姆病感染每年约30万美国公民。对基因调节的分子机制（包括涉及mRNA衰减的分子机制）的更好理解，这些病原体用于生存载体和宿主之间的过渡对于改善诊断和治疗策略的设计至关重要。

项目成果

Transcript decay mediated by RNase III in Borrelia burgdorferi.

伯氏疏螺旋体中由 RNase III 介导的转录本衰减。

• DOI: 10.1016/j.bbrc.2020.05.201
• 发表时间: 2020
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Snow,Santina;Bacon,Emily;Bergeron,Jennifer;Katzman,David;Wilhelm,Amelia;Lewis,Owen;Syangtan,Deepsing;Calkins,Andrew;Archambault,Linda;Anacker,MelissaL;Schlax,PaulaJean
• 通讯作者: Schlax,PaulaJean