TRANSLATIONAL REGULATION IN DIVERSE BACTERIAL SPECIES

不同细菌物种的翻译调控

• 批准号: 8360309
• 负责人: PAULA J SCHLAX
• 金额: $ 14.06万
• 依托单位: MOUNT DESERT ISLAND BIOLOGICAL LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360309
• 关键词: Bacteria; Bacteria sigma factor KatF protein; Biological Assay; Borrelia burgdorferi; Cells; DNA-Directed RNA Polymerase; Escherichia coli; Event; Functional RNA; Funding; Genes; Goals; Grant; Length; Lyme Disease; Maine; Maps; Messenger RNA; National Center for Research Resources; Operon; Principal Investigator; Prochlorococcus; Prokaryotic Cells; Proteins; Regulation; Research; Research Infrastructure; Resources; Ribosomal Proteins; Role; Sigma Factor; Source; Structure; Synechococcus; Trans-Activators; Translational Regulation; United States National Institutes of Health; Virulence; comparative; cost; environmental change; functional genomics; in vitro Assay; in vivo; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this proposed research is to explore similarities and differences in the translational regulation of a subunit of RNA polymerase and a ribosomal protein operon across diverse prokaryotes. The rpoS gene, encoding the alternative sigma factor sigma s, which influences bacterial virulence, is translationally regulated as a response to environmental changes in both Escherichia coli and Borrelia burgdorferi, the causative agent of Lyme disease. The roles of B. burgdorferi rpoS mRNA sequence, length and secondary structure, and the roles of trans-acting regulators, in particular a small non-coding RNA molecule, on translational initiation events will be characterized. In E. coli, the four ribosomal proteins of the alpha operon are translationally repressed by ribosomal protein S4, one of the proteins encoded in the operon. In Prochlorococcus marinus, Synechococcus sp. WH8102 and B. burgdorferi, rpsD, encoding S4, maps outside of the operon, suggesting a different mechanism of regulation. Both in vitro assays, using cell free extracts, and in vivo expression assays will be used to identify the cis-acting and trans-acting factors important in the translational regulation of the ¿ operon in these diverse bacteria and to elucidate their mechanism of translational regulation.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 这项拟议的研究的目的是探索RNA聚合酶亚基的转化调节和跨潜水员原核生物的核糖体蛋白歌剧的相似性和差异。 RPOS基因编码影响细菌病毒的替代Sigma因子Sigma S被翻译为对莱姆病的灾难性药物的大肠杆菌和Borrelia burgdorferi的响应。将表征Brgdorferi RPOS mRNA序列，长度和二次结构的作用，以及跨作用调节剂的作用，特别是一个小的非编码RNA分子在翻译起始事件中的作用。在大肠杆菌中，α操纵子的四个核糖体蛋白被核糖体蛋白S4抑制，核糖体蛋白S4是操纵子中编码的蛋白质之一。在核球菌中，Syechococcus sp。 WH8102和B. burgdorferi，RPSD，编码S4，在歌剧之外地图，提出了不同的调节机制。在体外测定中，使用无细胞提取物以及体内表达测定法都将用于确定在这些潜水细菌中– o operaon的转化调节中的顺式作用和反式作用因素，并阐明其翻译调节机制。