Effect of hypoxia on glucose metabolism in IUGR fetal liver

缺氧对IUGR胎肝糖代谢的影响

• 批准号: 8769415
• 负责人: Stephanie R Wesolowski
• 金额: $ 7.75万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8769415
• 关键词: Affect; Birth; Chronic; Clinical; Data; Development; Diabetes Mellitus; Disease; Elderly; Ensure; Exposure to; Fetal Growth; Fetal Growth Retardation; Fetal Liver; Fetal Mortality Statistics; Fetus; Figs - dietary; Gene Expression; Gene Silencing; Gene Targeting; Gluconeogenesis; Glucose; Glycolysis; Goals; Hepatic; Hepatocyte; Hypoglycemia; Hypoxia; Hypoxia Inducible Factor; IGFBP1 gene; Insulin; Insulin Resistance; Lead; Life; Link; Liver; Longevity; Measurement; Measures; Mediating; Metabolic; Metabolic Diseases; Metabolism; Molecular; Morbidity - disease rate; Neonatal Mortality; Non-Insulin-Dependent Diabetes Mellitus; Nuclear; Nutrient; Pathway interactions; Phosphorylation; Placenta; Placental Insufficiency; Play; Post-Translational Protein Processing; Predisposition; Pregnancy; Public Health; Regulation; Research; Resistance; Risk; Role; Sheep; Small Interfering RNA; Testing; VEGFA gene; Work; adverse outcome; fetal; fetal programming; fetus hypoxia; glucose metabolism; glucose production; hepatic gluconeogenesis; in utero; insulin sensitivity; liver metabolism; novel; novel strategies; offspring; oxidation; public health relevance; response

DESCRIPTION (provided by applicant): The fetal liver in intrauterine growth restricted pregnancies caused by placental insufficiency (PI-IUGR) develops metabolic adaptations to hypoxia and decreased placenta-to-fetus nutrient transfer. These adaptations include an early activation of gluconeogenesis and resistance to insulin's suppression of hepatic glucose production. This is important because while these metabolic adaptations may be necessary to ensure fetal survival, they also can lead to pathological conditions later in life, including type diabetes (T2DM). Importantly, the early mechanisms responsible for these metabolic reprogramming adaptations in the PI-IUGR fetal liver are largely unknown. Our working hypothesis is that hypoxia from placental insufficiency induces metabolic reprogramming, defined by increased glycolysis and decreased glucose oxidation, via a HIF and FOXO1 mechanism that also results in impaired insulin suppression of glucose production in the PI-IUGR fetal liver. The goal of this proposal is to determine the effect of hypoxia via a HIF-FOXO1 pathway that produces metabolic reprogramming and development of insulin resistance in fetal sheep hepatocytes. To test this proposed pathway, isolated hepatocytes from normal and PI-IUGR fetal sheep will be exposed to normoxic and hypoxic conditions. The expression of genes in gluconeogenesis, glycolysis, and glucose oxidation and other HIF and FOXO1 target genes will be measured to assess the effect of hypoxia on the induction of metabolic reprogramming. Glucose production rates with and without insulin will be measured to assess insulin sensitivity. FOXO1 activation and localization will be measured to determine the effect of hypoxia and insulin sensitivity. Gene silencing will be used to test the role of FOXO1. Overall, these results will demonstrate whether a novel mechanism between HIF and FOXO1 contributes to changes in fetal hepatic glucose metabolism and insulin resistance resulting from chronic hypoxia during PI-IUGR. These results will have significant scientific and potential clinical impact because IUGR affects up to 6-10% of pregnancies, yet it remains unclear how IUGR offspring develop increased risk for metabolic disease in later life. A link between early exposure to hypoxia, along with other nutrient deficiencies, during PI-IUGR and increased hepatocellular nuclear FOXO1 activity during fetal life may hold the key to understanding how PI-IUGR promotes the development of hepatic insulin resistance and dysregulated hepatic glucose production, which underlie later life development of T2DM. Overall, these new R03 studies combined with our K01 results will provide a detailed framework describing the coordinated changes in hepatic metabolism that develop in utero during PI- IUGR and predispose the liver to metabolic problems after birth.

描述（由申请人提供）：胎盘功能不全引起的宫内生长受限妊娠（PI-IUGR）的胎肝对缺氧和胎盘至胎儿营养转移减少产生代谢适应。这些适应包括肝细胞生成的早期激活和对胰岛素抑制肝葡萄糖产生的抵抗。这很重要，因为虽然这些代谢适应可能是确保胎儿存活所必需的，但它们也可能导致生命后期的病理状况，包括2型糖尿病（T2 DM）。重要的是，PI-IUGR胎儿肝脏中这些代谢重编程适应的早期机制在很大程度上是未知的。我们的工作假设是胎盘功能不全引起的缺氧通过HIF和FOXO 1机制诱导代谢重编程，其定义为糖酵解增加和葡萄糖氧化减少，该机制也导致PI-IUGR胎肝中葡萄糖产生的胰岛素抑制受损。本提案的目的是通过HIF-FOXO 1途径确定缺氧的影响，该途径在胎羊肝细胞中产生代谢重编程和胰岛素抵抗的发展。为了测试这一拟议的途径，将来自正常和PI-IUGR胎羊的分离肝细胞暴露于常氧和缺氧条件下。将测量胚胎发生、糖酵解和葡萄糖氧化中的基因以及其他HIF和FOXO 1靶基因的表达，以评估缺氧对诱导代谢重编程的影响。将测量加和不加胰岛素的葡萄糖生成率，以评估胰岛素敏感性。将测量FOXO 1活化和定位以确定缺氧和胰岛素敏感性的影响。基因沉默将用于测试FOXO 1的作用。总之，这些结果将证明HIF和FOXO 1之间的新机制是否有助于PI-IUGR期间慢性缺氧引起的胎儿肝脏葡萄糖代谢和胰岛素抵抗的变化。这些结果将具有重大的科学和潜在的临床影响，因为IUGR影响高达6-10%的妊娠，但目前尚不清楚IUGR后代如何在以后的生活中增加代谢疾病的风险。早期暴露于缺氧，沿着 在PI-IUGR期间与其他营养缺乏的关系以及胎儿期肝细胞核FOXO 1活性增加可能是理解PI-IUGR如何促进肝脏胰岛素抵抗和肝脏葡萄糖产生失调的关键，这是T2 DM后期发展的基础。总的来说，这些新的R 03研究结合我们的K 01结果将提供一个详细的框架，描述PI-IUGR期间子宫内发生的肝脏代谢的协调变化，并使肝脏在出生后易发生代谢问题。