Core B Flow Cytometry & Cell Sorting

核心 B 流式细胞术

• 批准号: 8874796
• 负责人: Qizhi Tang
• 金额: $ 20.95万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8874796
• 关键词: Adoptive Transfer; Animals; Back; Cell Culture System; Cell Separation; Cells; Communities; Computer software; Cytometry; Development; Diabetes Mellitus; Direct Costs; Disease; Disease Progression; Economics; Ensure; Equipment; Event; Experimental Designs; Failure; Flow Cytometry; Fluorescent Dyes; Funding; Funding Agency; Hour; Human Resources; Individual; Institution; Knowledge; Laboratories; Lasers; Learning; Life; Maintenance; Methods; Molecular Analysis; Molecular Profiling; Physiological; Population; Price; Protocols documentation; Quality Control; Reagent; Relative (related person); Research; Research Personnel; Research Project Grants; Resources; Services; Sorting - Cell Movement; Speed; Staining method; Stains; Techniques; Technology; Time; Training; United States National Institutes of Health; Work; abstracting; base; cell type; cost; experience; fluorescence activated cell sorter device; innovation; instrument; instrumentation; investigator training; meetings; member; molecular marker; novel; programs; protein profiling; repository; research study; response; sound; technology development; type I and type II diabetes

Abstract/Summary Core B: Flow Cytometry & Cell Sorting Core. The initiation and progression of events leading to type 1 and type 2 diabetes is accompanied by alterations in multiple cell types within the subject. Characterization, tracking and isolation of those cell populations by DRC investigators are enabled by the instruments and expertise available in the Flow Cytometry & Cell Sorting Core. Purpose: The Flow Cytometry & Cell Sorting Core assists investigators whose research requires the characterization of molecular markers in dispersed cells and/or the isolation of cells based on those markers. The Core provides the following services that is currently averaging 5501 accesses and 9419 cytometry/sorting hours by 143 researchers in 20 DRC laboratories: 1. Flow Cytometry. Five high-speed, multi-laser flow cytometers and analysis programs with highly complementary capabilities are available to meet DRC demand for cell characterization. 2. Cell Sorting. Six high-speed, multi-laser fluorescence activated cell sorters enable DRC investigators to isolate cell populations that can then be extensively characterized molecularly or that may be re-introduced to host animals to examine the effect of those cells on disease progression. 3. Information and Training. The Core trains investigators on the proper use of equipment and provides advice on the application of the technology. Benefits to DRC Community: The Core accelerates diabetes research by providing DRC investigators with access to advanced technologies and operational expertise that are beyond the abilities of individual laboratories to maintain. 39 NIH-funded and 31 other diabetes-related projects totaling $13,640,397 annual direct costs have benefited from the practical and economic access to this highly needed technology. Technology Development: Few laboratories have the resources to develop the most advanced capabilities to realize the full potential of the instruments. Support from the DRC enables one Core staff member to spend up to one day a week developing advanced capabilities that the DRC Executive Committee finds to be required by large numbers of DRC investigators. For example, new spectral analysis capabilities on advanced instruments not yet publicly available are now being developed in the Core to increase the accuracy and number of channels available for the analysis of specific cellular populations during disease progression. In the past three years, this Core has been successfully consolidated with other UCSF cytometry and cell sorting facilities to coordinate equipment procurement, expand instrument access and enhance staff availability. A robust co-pay mechanism ensures that DRC support is channeled to diabetes research in an institution-wide Core that provides access to a much greater degree of complementary instrumentation at lowered costs through the defraying of Core maintenance and personnel costs over a larger user base..

摘要/摘要 核心B：流式细胞仪和细胞排序核心。导致1型和类型的事件的启动和发展 2型糖尿病伴随着受试者中多种细胞类型的改变。特征， 刚果民主共和国调查人员对这些细胞群体的跟踪和隔离是由工具和 流式细胞仪和细胞排序核心中可用的专业知识。 目的：流式细胞仪和细胞分类核心核心协助研究人员的研究需要 根据这些标记物的分散细胞和/或细胞分离的分子标记表征。 核心提供以下服务，当前平均访问5501访问和9419个细胞仪/排序 143位研究人员在20个刚果民主共和国实验室中的小时： 1。流式细胞仪。具有高度的高速，多激光流动细胞仪和分析程序具有高度 可以使用互补功能来满足DRC对细胞表征的需求。 2。细胞分类。六个高速，多激荧光激活的细胞分类器使DRC研究者能够到达 分离的细胞群，然后可以广泛表征分子或可以重新引入的细胞群 宿主动物检查这些细胞对疾病进展的影响。 3。信息和培训。核心培训调查人员适当使用设备并提供 有关应用技术的建议。 刚果民主共和国社区的好处：核心通过为刚果民主共和国调查人员提供 访问超出个人能力的高级技术和运营专业知识 维护实验室。 39名NIH资助和其他31个与糖尿病相关的项目总计$ 13,640,397年 直接成本受益于对这项急需的技术的实际和经济获取。 技术开发：很少有实验室能够为开发最先进的功能提供资源 意识到乐器的全部潜力。刚果民主共和国的支持使一名核心工作人员能够支出 每周有一天，开发了刚果民主共和国执行委员会认为要求的高级功能 大量的刚果民主共和国调查员。例如，高级仪器的新光谱分析功能 现在尚未公开开发以提高准确性和数量的核心 可用于分析疾病进展过程中特定细胞种群的通道。 在过去的三年中，该核心已与其他UCSF细胞术和细胞成功合并 分类设施以协调设备采购，扩展工具访问并增强员工 可用性。强大的共付款机制可确保将DRC支持引导到糖尿病研究中 范围内的机构核心，可访问更大程度的互补仪器 通过在更大的用户群上支付核心维护和人员成本来降低成本。