Cytometry and Cell Sorting

细胞计数和细胞分选

• 批准号: 7925412
• 负责人: Qizhi Tang
• 金额: $ 12.92万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7925412
• 关键词: Calibration; Cell Separation; Cells; Color; Communities; Cytometry; Data; Detection; Disease Progression; Education; Ensure; Equipment; Experimental Designs; Flow Cytometry; Hand; Hour; Human Resources; Individual; Intervention; Laboratories; Life; Link; Maintenance; Population; Property; Protocols documentation; Quality Control; Reproducibility; Research; Research Personnel; Resources; Scientist; Services; Sorting - Cell Movement; Standardization; Training; Work; base; cost; improved; instrument; instrumentation; molecular marker; response; sound; technology development

Purpose: The DERC Cytometry & Cell Sorting Core assists investigators whose research requires the characterization of molecular markers in dispersed cells and/or the isolation of cells based on those markers Components: 1. Flow Cytometers. The LSRII enables simultaneous detection of 9 fluorescent colors on a single cell for thorough, quantitative characterization of large populations of cells. 2. Cell Sorters. Up to 25,000 cells per minute can be collected on the MoFlo and FACSAria cell sorters within the Core. The MoFlo is exclusively service-oriented whereas users can be trained to operate the FACSAria independently. 3. Assistance and Training. Core personnel are experts who can assist novice and more routine investigators either through direct, hands-on assistance or through proper training in equipment use and data interpretation. Benefits to DERC Community: The highly specialized flow cytometry and cell sorting equipment requires dedicated scientists to maintain and operate. Those same Core personnel are experts who can assist novice and more routine investigators. The availability of this expertise ensures sound experimental designs, expert advice and proper analysis. Technology Development: The instrumentations are highly sophisticated. In order to reach their full potential, they must be accurately and quantitatively calibrated. Improved capabilities will be implemented within the next DERC cycle in order to provide improved service that individual laboratories would struggle to achieve and maintain. Plans to expand service also include plans to increase capacity through the upgrade of existing instruments and the purchase of new ones.

目的：DERC细胞仪和细胞分类核心协助研究者的研究需要根据这些标记成分的分子分散细胞中分子标记和/或细胞分离的表征： 1。流式细胞仪。 LSRII可以同时检测单个细胞上的9种荧光颜色，以彻底，定量表征大量细胞。 2。细胞分类器。每分钟最多可在核心内的MOFLO和FACSARIA细胞分类器上收集多达25,000个细胞。 MOFLO专门以服务为导向，而可以培训用户独立操作Facsaria。 3。协助和培训。核心人员是专家，可以通过直接，动手帮助或通过对设备使用和数据解释进行适当培训来帮助新手和更多常规调查员。 对DERC社区的好处： 高度专业的流式细胞仪和细胞分选设备需要专门的科学家维护和运行。那些相同的核心人员是可以协助新手和更多常规调查人员的专家。这种专业知识的可用性可确保合理的实验设计，专家建议和适当的分析。 技术发展： 仪器非常复杂。为了发挥全部潜力，它们必须进行准确和定量校准。将在下一个DERC周期内实施改进的功能，以提供各个实验室将难以实现和维护的改进服务。扩展服务的计划还包括通过升级现有工具和购买新工具来提高产能的计划。