Cytometry and Cell Sorting

目的：DERC细胞测量和细胞分选核心帮助研究人员，他们的研究需要描述分散细胞中的分子标记和/或根据这些标记成分分离细胞： 1.流式细胞仪。LSRII能够在单个细胞上同时检测9种荧光颜色，以对大量细胞进行全面、定量的表征。 2.细胞分类器。在Core内的MoFlo和FACSAria细胞分选机上，每分钟可以收集多达25,000个细胞。MoFlo完全是面向服务的，而用户可以接受培训，独立操作FACSAria。 3.援助和培训。核心人员是能够通过直接、实际协助或通过在设备使用和数据解释方面的适当培训来协助新手和更常规的调查人员的专家。 给DERC社区带来的好处： 高度专业化的流式细胞仪和细胞分选设备需要专门的科学家进行维护和操作。这些核心人员都是专家，可以帮助新手和更常规的调查人员。这些专业知识的提供确保了合理的实验设计、专家建议和适当的分析。 技术开发： 这些仪器是非常精密的。为了充分发挥它们的潜力，必须对它们进行准确和定量的校准。将在下一个DERC周期内实施改进的能力，以便提供个别实验室难以实现和维持的改进服务。扩大服务的计划还包括通过升级现有仪器和购买新仪器来提高能力的计划。

Purpose: The DERC Cytometry & Cell Sorting Core assists investigators whose research requires the characterization of molecular markers in dispersed cells and/or the isolation of cells based on those markers Components: 1. Flow Cytometers. The LSRII enables simultaneous detection of 9 fluorescent colors on a single cell for thorough, quantitative characterization of large populations of cells. 2. Cell Sorters. Up to 25,000 cells per minute can be collected on the MoFlo and FACSAria cell sorters within the Core. The MoFlo is exclusively service-oriented whereas users can be trained to operate the FACSAria independently. 3. Assistance and Training. Core personnel are experts who can assist novice and more routine investigators either through direct, hands-on assistance or through proper training in equipment use and data interpretation. Benefits to DERC Community: The highly specialized flow cytometry and cell sorting equipment requires dedicated scientists to maintain and operate. Those same Core personnel are experts who can assist novice and more routine investigators. The availability of this expertise ensures sound experimental designs, expert advice and proper analysis. Technology Development: The instrumentations are highly sophisticated. In order to reach their full potential, they must be accurately and quantitatively calibrated. Improved capabilities will be implemented within the next DERC cycle in order to provide improved service that individual laboratories would struggle to achieve and maintain. Plans to expand service also include plans to increase capacity through the upgrade of existing instruments and the purchase of new ones.