Cytometry and Cell Sorting

细胞计数和细胞分选

• 批准号: 7925412
• 负责人: Qizhi Tang
• 金额: $ 12.92万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7925412
• 关键词: Calibration; Cell Separation; Cells; Color; Communities; Cytometry; Data; Detection; Disease Progression; Education; Ensure; Equipment; Experimental Designs; Flow Cytometry; Hand; Hour; Human Resources; Individual; Intervention; Laboratories; Life; Link; Maintenance; Population; Property; Protocols documentation; Quality Control; Reproducibility; Research; Research Personnel; Resources; Scientist; Services; Sorting - Cell Movement; Standardization; Training; Work; base; cost; improved; instrument; instrumentation; molecular marker; response; sound; technology development

Purpose: The DERC Cytometry & Cell Sorting Core assists investigators whose research requires the characterization of molecular markers in dispersed cells and/or the isolation of cells based on those markers Components: 1. Flow Cytometers. The LSRII enables simultaneous detection of 9 fluorescent colors on a single cell for thorough, quantitative characterization of large populations of cells. 2. Cell Sorters. Up to 25,000 cells per minute can be collected on the MoFlo and FACSAria cell sorters within the Core. The MoFlo is exclusively service-oriented whereas users can be trained to operate the FACSAria independently. 3. Assistance and Training. Core personnel are experts who can assist novice and more routine investigators either through direct, hands-on assistance or through proper training in equipment use and data interpretation. Benefits to DERC Community: The highly specialized flow cytometry and cell sorting equipment requires dedicated scientists to maintain and operate. Those same Core personnel are experts who can assist novice and more routine investigators. The availability of this expertise ensures sound experimental designs, expert advice and proper analysis. Technology Development: The instrumentations are highly sophisticated. In order to reach their full potential, they must be accurately and quantitatively calibrated. Improved capabilities will be implemented within the next DERC cycle in order to provide improved service that individual laboratories would struggle to achieve and maintain. Plans to expand service also include plans to increase capacity through the upgrade of existing instruments and the purchase of new ones.

目的：DERC 细胞计数和细胞分选核心为研究人员提供帮助，其研究需要表征分散细胞中的分子标记和/或基于这些标记分离细胞。 1.流式细胞仪。 LSRII 能够同时检测单个细胞上的 9 种荧光颜色，从而对大量细胞进行彻底的定量表征。 2. 细胞分选仪。 Core 内的 MoFlo 和 FACSAria 细胞分选仪每分钟可收集多达 25,000 个细胞。 MoFlo 完全以服务为导向，而用户可以接受培训以独立操作 FACSAria。 3. 协助和培训。核心人员是专家，他们可以通过直接的实践协助或通过设备使用和数据解释方面的适当培训来帮助新手和更常规的调查人员。 对 DERC 社区的好处： 高度专业化的流式细胞术和细胞分选设备需要专门的科学家来维护和操作。这些核心人员都是专家，可以帮助新手和更常规的调查人员。这种专业知识的可用性确保了良好的实验设计、专家建议和正确的分析。 技术开发： 仪器仪表非常精密。为了充分发挥其潜力，必须对它们进行准确和定量的校准。改进的能力将在下一个 DERC 周期内实施，以提供单个实验室难以实现和维持的改进服务。扩大服务的计划还包括通过升级现有仪器和购买新仪器来增加容量的计划。