BD FACS ARIA II CELL SORTER

BD FACS ARIA II 细胞分选仪

• 批准号: 7794706
• 负责人: Qizhi Tang
• 金额: $ 49.51万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794706
• 关键词: Aerosols; Animal Model; Applications Grants; Asthma; California; Cancer Immunology Science; Cell Separation; Cells; Clinical; Communities; Containment; Custom; Development; Devices; Diabetes Mellitus; Ensure; Equipment; Flow Cytometry; Fluorescence; Funding; Hour; Housing; Human; Laboratories; Laboratory Research; Lasers; Maintenance; Molecular; Process; Research; Research Personnel; Research Project Grants; Research Support; Safety; Sampling; San Francisco; Speed; Transplantation; Tube; United States National Institutes of Health; Universities; advanced system; base; career; design; experience; flexibility; fluorescence activated cell sorter device; improved; instrument; meetings; pathogen; photomultiplier; programs; public health relevance; research and development; response; user-friendly

DESCRIPTION (provided by applicant): This Shared Instrument Grant application is for the purchase of a high-speed fluorescence-activated cell sorter, FACSAria II by Becton Dickinson. This instrument is peerless in its user friendly design and offers maximal flexibility to adjust to various experimental needs; thus it is an ideal choice for user-operated shared equipment. We have custom configured the FACSAria II to have four lasers at 405 nm, 488 nm, 561 nm, and 640 nm and twelve photomultiplier tubes for simultaneous analysis of ten fluorescent parameters. The custom FACSAria II also comes with a fully enclosed fluidic system and an advanced aerosol management option that meets the safety standard for processing samples contaminated with human pathogens. The impetus for this application is insufficient cell sorting capacity currently available, increased cell sorting demand, and the lack of appropriately configured devices for new research developments at the University of California, San Francisco (UCSF). The new instrument has a projected usage of over 2500 hours per year by fifteen defined investigators, demonstrating a real need for this instrument. Six of the investigators are new and early-career investigators, two are new to UCSF Parnassus campus, and three are new users of cell sorters due to new project development; together these new activities represent over 70% of the projected use of this instrument. These new activities cannot be fully accommodated by existing, already oversubscribed sorters at UCSF. Furthermore, clinical and human sample processing represent over 40% of the estimated usage of this machine. Lastly, in addition to the general shortage of the sorters, there is very limited capacity to sort cells based on red fluorescence due to the lack of green lasers on existing sorters. In response to these needs, we have configured the new sorter with improved aerosol containment for human sample processing and an additional yellow-green laser for sorting cells with red fluorescent tags. Thus, the new sorter is tailored to maximally meet new research demands at UCSF. Researchers directly benefiting from this instrument are from five departments in diverse fields ranging from molecular and cellular research in animal models to translational and clinical projects. This instrument will directly support research projects by 9 investigators with active NIH funding and 6 new early career investigators. The new sorter will be jointly managed by the Laboratory of Cell Analysis (LCA) and the Transplantation Research Laboratory (TRL). The LCA is a campus-wide core program that has over sixty years of experience in flow cytometry support. It will be responsible for technical and administrative support of the instrument. The TRL will house the instrument and provide backup onsite technical support. The new instrument will be integrated into the existing network of sorters managed by LCA to ensure expert maintenance of the instrument. Coordinated management of similar equipment will also ensure consistency in sorter recharge rate and access across the campus, which will likely benefit the UCSF research community at large. PUBLIC HEALTH RELEVANCE: We are requesting a high-speed Sorter to be shared by more than 15 researchers at UCSF Parnassus campus. This instrument will support research in a wide range of research fields such as Transplantation, Diabetes, Asthma, Cancer, and Immunology.

描述（由申请人提供）：此共享仪器拨款申请用于购买 Becton Dickinson 生产的高速荧光激活细胞分选仪 FACSAria II。该仪器具有无与伦比的用户友好设计，并提供最大的灵活性来适应各种实验需求；因此它是用户操作共享设备的理想选择。我们定制配置了 FACSAria II，使其具有 405 nm、488 nm、561 nm 和 640 nm 的四个激光器以及十二个光电倍增管，用于同时分析十个荧光参数。定制的 FACSAria II 还配备了全封闭流体系统和先进的气溶胶管理选项，满足处理受人类病原体污染的样品的安全标准。 该应用的推动力是当前可用的细胞分选能力不足、细胞分选需求增加以及加州大学旧金山分校 (UCSF) 缺乏用于新研究开发的适当配置的设备。预计 15 名指定研究人员每年会使用该新仪器超过 2500 小时，这表明了对该仪器的真正需求。其中六名研究人员是新的和早期职业研究人员，两名是加州大学旧金山分校帕纳苏斯校区的新人，三名是由于新项目开发而使用细胞分选仪的新用户；这些新活动合计占该工具预计使用量的 70% 以上。加州大学旧金山分校现有的、已经超额认购的分拣机无法完全满足这些新活动。此外，临床和人体样本处理占该机器预计使用量的 40% 以上。最后，除了分选机普遍短缺之外，由于现有分选机缺乏绿色激光器，基于红色荧光的细胞分选能力也非常有限。为了满足这些需求，我们为新型分选机配置了改进的气溶胶遏制能力，用于人体样本处理，并配备了额外的黄绿色激光器，用于分选带有红色荧光标签的细胞。因此，新的分拣机是为了最大限度地满足加州大学旧金山分校的新研究需求而量身定制的。直接受益于该仪器的研究人员来自不同领域的五个部门，从动物模型的分子和细胞研究到转化和临床项目。该工具将直接支持 9 名获得 NIH 资助的研究人员和 6 名新的早期职业研究人员的研究项目。 新的分选机将由细胞分析实验室（LCA）和移植研究实验室（TRL）共同管理。 LCA 是一个全校范围的核心计划，在流式细胞术支持方面拥有六十多年的经验。它将负责该文书的技术和行政支持。 TRL 将安置该仪器并提供备用现场技术支持。新仪器将集成到 LCA 管理的现有分拣机网络中，以确保仪器的专家维护。对类似设备的协调管理还将确保整个校园内分拣机充电率和访问的一致性，这可能会让整个加州大学旧金山分校的研究界受益。 公共健康相关性：我们请求 UCSF Parnassus 校区超过 15 名研究人员共享一台高速分拣机。该仪器将支持移植、糖尿病、哮喘、癌症和免疫学等广泛研究领域的研究。