Core B Flow Cytometry & Cell Sorting

摘要/摘要 核心B：流式细胞术和细胞分选核心。导致类型1和类型1的事件的开始和发展 2型糖尿病伴有受试者体内多种细胞类型的改变。塑造人物形象， 由DRC调查人员跟踪和分离这些细胞群是通过这些仪器和 在流式细胞术和细胞分选核心中提供专业知识。 目的：流式细胞术和细胞分选核心帮助研究需要 分散细胞中分子标记的表征和/或基于这些标记的细胞分离。 Core提供以下服务，目前平均有5501次访问和9419次细胞分析/分类 20个刚果民主共和国实验室的143名研究人员的工作时间： 1.流式细胞术。五台高速、多激光流式细胞仪和分析程序 可提供互补的功能，以满足DRC对细胞特性的需求。 2.细胞分选。六台高速、多激光荧光激活的细胞分选仪使DRC调查人员能够 分离细胞群体，然后可以广泛的分子特征或可能重新引入 以检测这些细胞对疾病进展的影响。 3.信息和培训。CORE培训调查人员正确使用设备，并提供 关于这项技术的应用的建议。 对DRC社区的好处：Core通过为DRC调查人员提供 获得超出个人能力的先进技术和运营专业知识 实验室需要维护。美国国立卫生研究院资助的39个糖尿病相关项目和31个其他糖尿病相关项目，每年总计13,640,397美元 直接成本得益于以实际和经济的方式获得这种迫切需要的技术。 技术开发：很少有实验室有资源来开发最先进的能力 充分发挥这些工具的潜力。来自刚果民主共和国的支持使一名核心员工能够 到每周一天，开发刚果民主共和国执行委员会认为需要的高级能力 大批刚果(金)调查人员。例如，先进仪器上的新光谱分析功能 现在正在开发核心，以提高准确性和数量 在疾病发展过程中可用于分析特定细胞群体的通道。 在过去的三年里，这一核心已经成功地与其他UCSF细胞仪和细胞 分类设施，以协调设备采购，扩大仪器通道，并加强员工 可用性。强大的自付机制确保DRC的支持在 机构范围的核心，在以下方面提供更大程度的补充工具 通过在更大的用户群中支付核心维护和人员成本来降低成本。

Abstract/Summary Core B: Flow Cytometry & Cell Sorting Core. The initiation and progression of events leading to type 1 and type 2 diabetes is accompanied by alterations in multiple cell types within the subject. Characterization, tracking and isolation of those cell populations by DRC investigators are enabled by the instruments and expertise available in the Flow Cytometry & Cell Sorting Core. Purpose: The Flow Cytometry & Cell Sorting Core assists investigators whose research requires the characterization of molecular markers in dispersed cells and/or the isolation of cells based on those markers. The Core provides the following services that is currently averaging 5501 accesses and 9419 cytometry/sorting hours by 143 researchers in 20 DRC laboratories: 1. Flow Cytometry. Five high-speed, multi-laser flow cytometers and analysis programs with highly complementary capabilities are available to meet DRC demand for cell characterization. 2. Cell Sorting. Six high-speed, multi-laser fluorescence activated cell sorters enable DRC investigators to isolate cell populations that can then be extensively characterized molecularly or that may be re-introduced to host animals to examine the effect of those cells on disease progression. 3. Information and Training. The Core trains investigators on the proper use of equipment and provides advice on the application of the technology. Benefits to DRC Community: The Core accelerates diabetes research by providing DRC investigators with access to advanced technologies and operational expertise that are beyond the abilities of individual laboratories to maintain. 39 NIH-funded and 31 other diabetes-related projects totaling $13,640,397 annual direct costs have benefited from the practical and economic access to this highly needed technology. Technology Development: Few laboratories have the resources to develop the most advanced capabilities to realize the full potential of the instruments. Support from the DRC enables one Core staff member to spend up to one day a week developing advanced capabilities that the DRC Executive Committee finds to be required by large numbers of DRC investigators. For example, new spectral analysis capabilities on advanced instruments not yet publicly available are now being developed in the Core to increase the accuracy and number of channels available for the analysis of specific cellular populations during disease progression. In the past three years, this Core has been successfully consolidated with other UCSF cytometry and cell sorting facilities to coordinate equipment procurement, expand instrument access and enhance staff availability. A robust co-pay mechanism ensures that DRC support is channeled to diabetes research in an institution-wide Core that provides access to a much greater degree of complementary instrumentation at lowered costs through the defraying of Core maintenance and personnel costs over a larger user base..