Use of Bacteriophages to Prevent, Diagnose, and Treat Diseases

靶向抗癌药物递送一种双展示噬菌体(T4)系统，其特点是外部有蛋白质标签，噬菌体内部有包装蛋白质，将用于向细胞运送治疗性蛋白质。外部标签将通过ICAM途径诱导内吞作用。内化的病毒将在溶酶体内运送蛋白质，允许通过预先存在的蛋白质相互作用(形成毛孔的毒素)或多赖氨酸残基(质子海绵效应)逃逸。从今年夏天开始，我们开始构建这样的噬菌体。噬菌体在癌症检测中的应用。我们之前已经开发了一种方法，通过使用量子点标记的噬菌体来检测临床样本中特定细菌的存在，这种噬菌体是针对给定细菌的。我们目前正在开发在噬菌体波长上展示单链抗体的方法，这种单链抗体是与给定的癌细胞结合的。我们将从可用于毛细胞白血病的单变抗体克隆开始。一旦构建完成，我们将使用我们之前开发的生物素化多肽来用量子点装饰相同的噬菌体。当这些噬菌体与癌细胞结合时，会使后者发出荧光。

Targeted cancer drug delivery a dual display phage (T4) system, featuring protein tags externally and packaged protein inside the phage, will be used to deliver therapeutic proteins to cells. The external tags will induce endocytosis through the ICAM pathway. The internalized virus will deliver proteins inside a lysosome, allowing escape through pre-existing protein interactions (toxins that form pores) or poly-lysine residues (the proton sponge effect). We have started constructing such phages beginning this summer. Use of bacteriophage in cancer detection. We have previously developed a method for detecting specific bacterial presence in clinical samples by the use of quantum dot labeled bacteriophage, which is specific for a given bacteria. We are currently developing ways to display on phage wavelength single chain antibody which is specific for binding on a given cancer cell. We will start with single change antibody clone available for Hairy Cell leaukemia. Once constructed we will decorate the same phage with quantum dots using biotinylated peptide that we have developed previously. These phages when bound to cancer cells will make the latter fluorescence.