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Instrumentation grant application for forteBio Octet Red96 Biolayer Interferometry System

forteBio Octet Red96 生物层干涉测量系统的仪器拨款申请

基本信息

批准号：
8826236
负责人：
Markus A Seeliger
金额：
$ 20.45万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-01 至 2016-01-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a forteBio OctetRed96 biolayer interferometer (BLI) for the study of molecular interactions. The BLI instrument uses a method for detection that is similar to surface plasmon resonance spectroscopy (SPR). The BLI instrument detects binding of ligands to immobilized receptors on a detector surface via an optical signal. Key features of the OctetRed96 are the ability to use unlabeled ligands, low sample consumption, a 96 well format, 8-channel parallel detection and the sensitivity to detect and analyze binding rates and affinities for small molecules to their receptors. The key advantages of BLI over SPR are the higher tolerance for solvents, the absence of maintenance- prone microfluidics and the lower cost of purchase and operation. Currently, there is no instrument available at Stony Brook University or another nearby institution to quantify binding affinity and rates of unlabeled small molecules. The OctetRed96 will enable ligand binding screens for drug-lead development efforts with a throughput of about 100 compounds per day. Furthermore, the instrument will allow the participating researchers to quantify the affinities and association rates of protein-protein, protein-peptide, protein-nucleic acid and protein-lipid interactions to explore fundamental biological problems related to human disease. Management and operation of the system will be provided by a technical specialist, who will also assist users in developing binding assays, as well as helping investigators in the analysis of the binding data.

描述（由申请人提供）：本申请申请购买forteBio OctetRed96生物层干涉仪（BLI）的资金，用于分子相互作用的研究。BLI仪器使用一种类似于表面等离子体共振光谱（SPR）的检测方法。BLI仪器通过光信号检测探测器表面上固定受体与配体的结合。OctetRed96的主要特点是能够使用未标记的配体，低样品消耗，96孔格式，8通道平行检测以及检测和分析小分子与其受体的结合率和亲和力的灵敏度。与SPR相比，BLI的主要优点是对溶剂的耐受性更高，没有易于维护的微流体，购买和操作成本更低。目前，石溪大学或附近的其他机构没有可用的仪器来量化未标记小分子的结合亲和力和速率。OctetRed96将为药物先导开发工作提供配体结合筛选，每天可处理约100种化合物。此外，该仪器将允许参与研究人员量化蛋白质-蛋白质、蛋白质-肽、蛋白质-核酸和蛋白质-脂质相互作用的亲和力和关联率，以探索与人类疾病相关的基本生物学问题。该系统的管理和操作将由一名技术专家提供，该专家还将协助用户开发结合分析方法，并帮助调查人员分析结合数据。