Instrumentation grant application for forteBio Octet Red96 Biolayer Interferometry System

forteBio Octet Red96 生物层干涉测量系统的仪器拨款申请

基本信息

项目摘要

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a forteBio OctetRed96 biolayer interferometer (BLI) for the study of molecular interactions. The BLI instrument uses a method for detection that is similar to surface plasmon resonance spectroscopy (SPR). The BLI instrument detects binding of ligands to immobilized receptors on a detector surface via an optical signal. Key features of the OctetRed96 are the ability to use unlabeled ligands, low sample consumption, a 96 well format, 8-channel parallel detection and the sensitivity to detect and analyze binding rates and affinities for small molecules to their receptors. The key advantages of BLI over SPR are the higher tolerance for solvents, the absence of maintenance- prone microfluidics and the lower cost of purchase and operation. Currently, there is no instrument available at Stony Brook University or another nearby institution to quantify binding affinity and rates of unlabeled small molecules. The OctetRed96 will enable ligand binding screens for drug-lead development efforts with a throughput of about 100 compounds per day. Furthermore, the instrument will allow the participating researchers to quantify the affinities and association rates of protein-protein, protein-peptide, protein-nucleic acid and protein-lipid interactions to explore fundamental biological problems related to human disease. Management and operation of the system will be provided by a technical specialist, who will also assist users in developing binding assays, as well as helping investigators in the analysis of the binding data.
描述(由申请人提供):该提案要求提供资金,用于购买forteBio OctetRed 96生物层干涉仪(BLI),用于分子相互作用的研究。BLI仪器使用类似于表面等离子体共振光谱(SPR)的检测方法。BLI仪器通过光学信号检测配体与检测器表面上固定的受体的结合。OctetRed 96的主要特点是能够使用未标记的配体、低样品消耗、96孔格式、8通道平行检测以及检测和分析小分子与其受体的结合率和亲和力的灵敏度。与SPR相比,BLI的关键优势在于对溶剂的耐受性更高,无需易于维护的微流体,以及购买和操作成本更低。目前,在斯托尼布鲁克大学或另一个附近的机构没有可用的仪器来量化未标记的小分子的结合亲和力和比率。OctetRed 96将使配体结合筛选用于药物先导开发工作,每天的吞吐量约为100种化合物。此外,该仪器将允许参与研究的研究人员量化蛋白质-蛋白质,蛋白质-肽,蛋白质-核酸和蛋白质-脂质相互作用的亲和力和缔合率,以探索与人类疾病相关的基本生物学问题。该系统的管理和操作将由一名技术专家提供,他还将协助用户开发结合测定法,并帮助研究人员分析结合数据。

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Elastase Digestion of Fibronectin Releases an Epiviosamine Peptide with Fibroblast Growth and Survival Activity.
纤维连接蛋白的弹性蛋白酶消化释放出具有成纤维细胞生长和存活活性的表维维胺肽。
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Markus A Seeliger其他文献

An allosteric add-on
变构附加物
  • DOI:
    10.1038/nchembio.1630
  • 发表时间:
    2014-09-07
  • 期刊:
  • 影响因子:
    13.700
  • 作者:
    Zachariah H Foda;Markus A Seeliger
  • 通讯作者:
    Markus A Seeliger

Markus A Seeliger的其他文献

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{{ truncateString('Markus A Seeliger', 18)}}的其他基金

Dynamics of inhibitor binding and regulation of protein tyrosine kinases
抑制剂结合动力学和蛋白酪氨酸激酶调节
  • 批准号:
    10385978
  • 财政年份:
    2016
  • 资助金额:
    $ 20.45万
  • 项目类别:
Dynamics of inhibitor binding and regulation of protein tyrosine kinases
抑制剂结合动力学和蛋白酪氨酸激酶调节
  • 批准号:
    9313905
  • 财政年份:
    2016
  • 资助金额:
    $ 20.45万
  • 项目类别:
Dynamics of inhibitor binding and regulation of protein tyrosine kinases
抑制剂结合动力学和蛋白酪氨酸激酶调节
  • 批准号:
    9925795
  • 财政年份:
    2016
  • 资助金额:
    $ 20.45万
  • 项目类别:
Dynamics of Ligand Binding and Protein Kinase Regulation
配体结合动力学和蛋白激酶调节
  • 批准号:
    10625416
  • 财政年份:
    2016
  • 资助金额:
    $ 20.45万
  • 项目类别:
Dynamics of Ligand Binding and Protein Kinase Regulation
配体结合动力学和蛋白激酶调节
  • 批准号:
    10204514
  • 财政年份:
    2016
  • 资助金额:
    $ 20.45万
  • 项目类别:
Dynamics of Ligand Binding and Protein Kinase Regulation
配体结合动力学和蛋白激酶调节
  • 批准号:
    10414000
  • 财政年份:
    2016
  • 资助金额:
    $ 20.45万
  • 项目类别:
SRC, A PROTEIN KINASE ACTIVE IN CHRONIC MYELOID LEUKEMIA
SRC,一种在慢性粒细胞白血病中活跃的蛋白激酶
  • 批准号:
    8363361
  • 财政年份:
    2011
  • 资助金额:
    $ 20.45万
  • 项目类别:
Conformational Dynamics of Protein Tyrosine Kinases Src and Abl
蛋白酪氨酸激酶 Src 和 Abl 的构象动力学
  • 批准号:
    8197751
  • 财政年份:
    2007
  • 资助金额:
    $ 20.45万
  • 项目类别:
Conformational Dynamics of Protein Tyrosine Kinases Src and Abl
蛋白酪氨酸激酶 Src 和 Abl 的构象动力学
  • 批准号:
    7319435
  • 财政年份:
    2007
  • 资助金额:
    $ 20.45万
  • 项目类别:
Conformational Dynamics of Protein Tyrosine Kinases Src and Abl
蛋白酪氨酸激酶 Src 和 Abl 的构象动力学
  • 批准号:
    7469423
  • 财政年份:
    2007
  • 资助金额:
    $ 20.45万
  • 项目类别:
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