权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Instrumentation grant application for forteBio Octet Red96 Biolayer Interferometry System

forteBio Octet Red96 生物层干涉测量系统的仪器拨款申请

基本信息

批准号：
8826236
负责人：
Markus A Seeliger
金额：
$ 20.45万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-01 至 2016-01-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a forteBio OctetRed96 biolayer interferometer (BLI) for the study of molecular interactions. The BLI instrument uses a method for detection that is similar to surface plasmon resonance spectroscopy (SPR). The BLI instrument detects binding of ligands to immobilized receptors on a detector surface via an optical signal. Key features of the OctetRed96 are the ability to use unlabeled ligands, low sample consumption, a 96 well format, 8-channel parallel detection and the sensitivity to detect and analyze binding rates and affinities for small molecules to their receptors. The key advantages of BLI over SPR are the higher tolerance for solvents, the absence of maintenance- prone microfluidics and the lower cost of purchase and operation. Currently, there is no instrument available at Stony Brook University or another nearby institution to quantify binding affinity and rates of unlabeled small molecules. The OctetRed96 will enable ligand binding screens for drug-lead development efforts with a throughput of about 100 compounds per day. Furthermore, the instrument will allow the participating researchers to quantify the affinities and association rates of protein-protein, protein-peptide, protein-nucleic acid and protein-lipid interactions to explore fundamental biological problems related to human disease. Management and operation of the system will be provided by a technical specialist, who will also assist users in developing binding assays, as well as helping investigators in the analysis of the binding data.

描述(由申请人提供)：本提案要求提供资金，用于购买ForteBio OcteRed96生物层干涉仪(BLI)，用于研究分子相互作用。BLI仪器使用一种类似于表面等离子体共振光谱(SPR)的检测方法。BLI仪器通过光学信号检测配体与检测器表面固定的受体的结合。OcteRed96的主要功能是能够使用未标记的配体、低样本消耗、96孔格式、8通道并行检测以及检测和分析小分子与其受体的结合率和亲和力的灵敏度。与SPR相比，BLI的主要优势是对溶剂的耐受性更高，不存在易于维护的微流体，并且购买和运行成本较低。目前，石溪大学或附近的其他机构还没有可用的仪器来量化结合亲和力和未标记小分子的速率。OctyRed96将为药物先导开发工作启用配体结合筛查，每天可处理约100种化合物。此外，该仪器将使参与研究人员能够量化蛋白质-蛋白质、蛋白质-多肽、蛋白质-核酸和蛋白质-脂肪相互作用的亲和力和关联率，以探索与人类疾病有关的基本生物学问题。该系统的管理和操作将由一名技术专家提供，他还将协助用户开发具有约束力的分析，并帮助调查人员分析具有约束力的数据。