Synaptic Vesicle Dynamics at Hippocampal Synapses

海马突触的突触小泡动力学

• 批准号: 9170987
• 负责人: THOMAS SCHIKORSKI
• 金额: $ 45.41万
• 依托单位: UNIVERSIDAD CENTRAL DEL CARIBE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2020-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9170987
• 关键词: Affect; Brain; Cell membrane; Cells; Characteristics; Clathrin; Coated vesicle; Communication; Complement; Electron Microscope; Electron Microscopy; Electrons; Endocytic Vesicle; Endocytosis; Endosomes; Equilibrium; Event; Exocytosis; Freezing; Heart; Hippocampus (Brain); Hispanic-serving Institution; Horseradish Peroxidase; Knowledge; Label; Lead; Mediating; Membrane; Mental Depression; Microscopic; Molecular; Morphology; Nature; Neurons; Neurotransmitters; Pathway interactions; Peripheral; Physiological; Play; Proteins; Reporter Genes; Reporting; Research; Retrieval; Role; Running; Shapes; Site; Speed; Synapses; Synaptic Vesicles; Techniques; Testing; Time; Vesicle; abstracting; base; coated pit; design; editorial; graduate student; in vivo; innovation; microwave electromagnetic radiation; minority undergraduate; molecular size; neurotransmitter release; new technology; postsynaptic; pressure; research study; restoration; sample fixation; synaptic function; tool; vesicular release

Abstract Regulated neurotransmitter release is the basis for neuronal communication. Neurotransmitter is stored in synaptic vesicles and released via fusion with the plasma membrane. Consequently, the dynamics that govern synaptic vesicle morphology and function are deterministic for synaptic function. Synaptic vesicle dynamics can be divided into three segments: fusion, retrieval, and restoration. This application proposes to characterize these segments by capturing synaptic vesicles at accurate times after synaptic vesicle fusion and visualizing their dynamic states in the electron microscope. This approach will be complemented with diverse labeling techniques and genetically encoded markers for electron microscopy.

摘要 神经递质的调节释放是神经元通讯的基础。神经递质 储存在突触小泡中，并通过与质膜融合释放。因此， 支配突触小泡形态和功能的动力学对 突触功能。突触小泡动力学可分为三个部分：融合， 检索和修复。该应用程序建议通过以下方式来描述这些细分市场 在突触囊泡融合后的准确时间捕获突触小泡并将其可视化 电子显微镜中的动态。这一方法将与不同的 电子显微镜的标记技术和遗传编码标记。