ORGANIZATION OF ACTIVE ZONE WITHIN PRESYNAPTIC TERMINALS ON HIPPOCAMPAL NEURONS
ORGANIZATION OF ACTIVE ZONE WITHIN PRESYNAPTIC TERMINALS ON HIPPOCAMPAL NEURONS
批准号:
6121825
负责人:
THOMAS SCHIKORSKI
金额:
$2.78万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-05-15 至 2000-04-30
中文摘要
虽然突触结构和功能之间的相关性
已经在神经肌肉接头处进行了详细研究,我们面临着许多
开放式问题。 中枢突触的了解甚至更少。
最近的研究表明,单个突触接触的大小
与其功能相关。 特别是囊泡的数量
停靠在活动区域似乎可以准确预测释放
该接触的概率。 然而,尚不清楚是否每个
囊泡对释放概率的贡献相同。 一个精确的
对接囊泡位置的绘图可能揭示功能
各个囊泡之间的差异。 因此,我们重构了
使用高压电子显微镜观察海马突触
断层扫描 3D 重建。 使用这个方法我们可以解决
每个囊泡的位置总共误差小于10 nm
尺寸,而连续切片的重建是有限的
z 轴分辨率降低 6 倍。 到目前为止,10个突触
已被重建。 对接的中心到中心距离
使用 NCMIR 开发的程序 xdsphere 计算囊泡
由 Stephan Lamont 编写,并使用配对相关函数进行分析。
该函数显示出复杂但有序的一致模式
活动区域之间。 这项工作在冬季大脑上发表
今年的会议在犹他州帕克城举行。
英文摘要
Although the correlation between synaptic structure and function
has been studied in detail at the neuromuscular junction, we face many
open questions. Central synapses are even less well understood.
Recent studies have shown that the size of a single synaptic contact
is correlated to its function. In particular the number of vesicles
docked at the active zone seems to accurately predict the release
probability of that contact. It is not clear, however, whether every
vesicle contributes equally to the release probability. An exact
mapping of the position of docked vesicles might reveal functional
differences between individual vesicles. We, therefore, reconstructed
hippocampal synapses by using high voltage electron microscopy and
tomographic 3-D reconstructions. Using this method we can resolve the
position of every vesicle with an error of less than 10 nm in all
dimensions, whereas reconstructions from serial sections are limited
to a 6 times lower resolution in the z-axis. Thus far, 10 synapses
have been reconstructed. The center to center distances of the docked
vesicles were calculated using the program xdsphere developed at NCMIR
by Stephan Lamont and analyzed with the pair correlation function.
The function shows a complex but orderly pattern that is consistent
between active zones. This work was presented at the Winter Brain
Conference this year in Park City, Utah.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Synaptic Vesicle Dynamics at Hippocampal Synapses
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批准号:9170987
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项目类别:
-
资助金额:$45.41万
-
财政年份:2016
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负责人:THOMAS SCHIKORSKI
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依托单位:
Synaptic Vesicle Dynamics at Excitatory Hippocampal Synapses
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批准号:8079279
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项目类别:
-
资助金额:$28.87万
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财政年份:2011
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负责人:THOMAS SCHIKORSKI
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依托单位:
Synaptic Vesicle Dynamics at Excitatory Hippocampal Synapses
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批准号:8469588
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项目类别:
-
资助金额:$28.23万
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财政年份:2011
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负责人:THOMAS SCHIKORSKI
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依托单位:
Synaptic Vesicle Dynamics at Excitatory Hippocampal Synapses
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批准号:8320864
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项目类别:
-
资助金额:$29.06万
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财政年份:2011
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负责人:THOMAS SCHIKORSKI
-
依托单位:
HRP cDNA expression: a label for modern electron microscopy on neuroscience
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批准号:7845476
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项目类别:
-
资助金额:$14.8万
-
财政年份:2009
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负责人:THOMAS SCHIKORSKI
-
依托单位:
ORGANIZATION OF ACTIVE ZONE WITHIN PRESYNAPTIC TERMINALS ON HIPPOCAMPAL NEURONS
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批准号:6469034
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项目类别:
-
资助金额:$10.66万
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财政年份:2001
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负责人:THOMAS SCHIKORSKI
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依托单位:
ORGANIZATION OF ACTIVE ZONE WITHIN PRESYNAPTIC TERMINALS ON HIPPOCAMPAL NEURONS
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批准号:6354285
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项目类别:
-
资助金额:$3.85万
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财政年份:2000
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负责人:THOMAS SCHIKORSKI
-
依托单位:
ORGANIZATION OF ACTIVE ZONE WITHIN PRESYNAPTIC TERMINALS ON HIPPOCAMPAL NEURONS
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批准号:6220673
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项目类别:
-
资助金额:$3.85万
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财政年份:1999
-
负责人:THOMAS SCHIKORSKI
-
依托单位:
ORGANIZATION OF ACTIVE ZONE WITHIN PRESYNAPTIC TERMINALS ON HIPPOCAMPAL NEURONS
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批准号:6282138
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项目类别:
-
资助金额:$2.95万
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财政年份:1998
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负责人:THOMAS SCHIKORSKI
-
依托单位:
ORGANIZATION OF ACTIVE ZONE WITHIN PRESYNAPTIC TERMINALS ON HIPPOCAMPAL NEURONS
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批准号:6252933
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项目类别:
-
资助金额:$1.9万
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财政年份:1997
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负责人:THOMAS SCHIKORSKI
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依托单位:
Electron Microscopy Research Core
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批准号:8585194
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项目类别:
-
资助金额:$13.43万
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财政年份:--
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负责人:THOMAS SCHIKORSKI
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依托单位:
Electron Microscopy Research Core
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批准号:8711578
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项目类别:
-
资助金额:$13.29万
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财政年份:--
-
负责人:THOMAS SCHIKORSKI
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依托单位:
海外基金