Neurolucida BrainMaker Imaging System

Neurolucida BrainMaker 成像系统

• 批准号: 9075950
• 负责人: Z JOSH HUANG
• 金额: $ 20.34万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075950
• 关键词: Behavior; Biological Sciences; Brain imaging; Cells; Communities; Development; Electrophysiology (science); Fluorescence Microscopy; Funding; Genetic Engineering; Laboratories; Modeling; Morphology; Mus; Neurons; Research Personnel; Research Support; Resolution; Rodent; System; Techniques; United States National Institutes of Health; Work; brain volume; image reconstruction; imaging system; neural circuit; neuroimaging; neuropsychiatric disorder; reconstruction

 DESCRIPTION (provided by applicant): Multiple NIH supported neuroscientists at Cold Spring Harbor Laboratory form a highly integrated community studying rodent neural circuit development and function and models of neuropsychiatric disorders. We integrate multiple approaches and techniques from mouse genetic engineering to state of the art neuroimaging, electrophysiology, and behavior analysis. A key component of neural circuit study is to visualize, reconstruct, and quantify neuronal morphology and connectivity at cellular and subcellular resolution and in large brain volume. Semi-automated fluorescence microscopy combined with Neurolucida serial reconstruction, quantification, and display has proven to be an effective system. Our current work station was acquired over 12 years ago, with very limited functionality and capacity. We are requesting funds to purchase a Neurolucida BrainMaker Imaging System from MBF Bioscience for cell resolution whole brain imaging and reconstruction. This will allow us to continue to carry out cutting edge neural circuit research and support multiple NIH funded researchers.

 描述(由申请人提供)：美国国立卫生研究院支持的多名神经学家在冷泉港实验室组成一个高度整合的社区，研究啮齿动物神经回路的发育和功能以及神经精神障碍的模型。我们集成了多种方法和技术，从小鼠基因工程到最先进的神经成像、电生理学和行为分析。神经回路研究的一个关键组成部分是在细胞和亚细胞分辨率以及大脑体积上可视化、重建和量化神经元的形态和连接。半自动荧光显微镜结合NeuroLucida序列重建、量化和显示已被证明是一种有效的系统。我们目前的工作站是12年前收购的，功能和容量非常有限。我们正在申请资金，从MBF Bioscience购买一台NeuroLucida BrainMaker成像系统，用于细胞分辨率、全脑成像和重建。这将使我们能够继续开展尖端神经电路研究，并支持NIH资助的多名研究人员。