CryoEM Data Collection Facility Consortium at NCMI

NCMI 的 CryoEM 数据收集设施联盟

• 批准号: 9000209
• 负责人: ESTHER BULLITT
• 金额: $ 105.48万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-15 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9000209
• 关键词: Academic Training; Accounting; Advisory Committees; Arbitration; Biological; Boston; Cells; Conflict (Psychology); Conflict of Interest; Critiques; Cryoelectron Microscopy; Data; Data Collection; Data Storage and Retrieval; Department chair; Detection; Development; Devices; Distant; Educational workshop; Electron Microscope; Electrons; Environment; Funding; Guns; Image; Imaging technology; Institution; Laboratories; Lead; Logistics; Mammalian Cell; Medical; Medical center; Medicine; Microscope; Mission; Missouri; Molecular Machines; Noise; Outcome; Participant; Persons; Phase; Policies; Productivity; Proteins; Protocols documentation; Qualifying; Readiness; Recommendation; Request for Applications; Research; Research Infrastructure; Research Personnel; Resolution; Resource Allocation; Resources; Sampling; Schedule; Scientist; Services; Signal Transduction; Specialist; Specimen; Strategic Planning; Structure; Texas; Time; Training; Training Activity; Training Programs; Travel; United States National Institutes of Health; Universities; Virginia; Virus; Visit; base; chaperonin; college; contrast imaging; cost; data acquisition; data management; detector; electron tomography; experience; flexibility; image processing; improved; instrument; instrumentation; macromolecule; meetings; member; microscopic imaging; movie; operation; particle; public health relevance; structural biology; symposium; technology development; tomography

 DESCRIPTION (provided by applicant): The National Center for Macromolecular Imaging (NCMI) at Baylor College of Medicine is the host laboratory offering to provide a portion of the time on their JEM3200FSC and JEM2200FS electron microscopes for a cryo-electron microscopy (cryoEM) and tomography (cryoET) data acquisition facility Consortium. Both instruments are equipped with a field emission gun and an in-column energy filter, while the JEM2200FS also has a Zernike phase plate attachment. Both instruments are currently equipped with DE20 and DE12 cameras operated in integrating mode. These microscopes have been used productively for a variety of biological specimens, including macromolecules, molecular machines and cells, at state-of-the-art resolution. Our facilities will be allocated for he proposed Consortium while taking into account the ongoing research in the host institution. Our lead PI, Wah Chiu is a recognized expert in pushing cryoEM and cryoET beyond current boundaries and has decades of experience in leading several NIH funded research centers and academic training programs. There are 11 participating institutions across the USA, led by investigators who have a research track record in cryoEM and/or cryoET. Day-to-day operation will be carried out by a part-time cryoEM/ET scientist and IT staff. Both in person and remote data collection protocols will be used for distant users. The Consortium will provide travel funds for the distant users. We request funding for a Direct Detection Device (DDD) operated in counting mode to meet the needs of all types of specimens. The host Institution will provide an administrative assistant to support user visits and various Consortium activities, and matching funds to purchase the DDD and adequate hard drives for short-term data storage. The participating PIs will be involved in formulating the specific policies for Consortium governance and developing training activities. Scheduling will be allocated evenly in the first year among participants with some flexibility based on specimen readiness. Allocation of microscope times and priorities will be assigned in the second year and beyond based on recommendations of an external microscope allocation committee appointed by all the PIs to avoid conflict of interest. Al PIs will meet quarterly on WebEx to discuss technical and administrative issues, and we will hold an annual user meeting. An annual review of all aspects of the Consortium operation and the data outcomes of the users will be conducted by an advisory committee to assure the highest productivity and usage of the proposed facility. The membership of the participating institutions will be dynamically reviewed by all the PIs with the inputs from the external advisory committee after the second year of our operation.

 描述（由适用提供）：贝勒医学院的国家大分子成像中心（NCMI）是主机实验室，旨在提供部分时间上的JEM3200FSC和JEM2200FS电子显微镜，用于冷冻电子显微镜（Cryo-Electron Microscopy（Cryoem）和Sometography（Cryoet）和Cryoet）（Cryoet）的数据可获得的功能。这两种仪器均配备了野外发射枪和列内能滤波器，而JEM2200FS也具有Zernike相板的附件。目前，这两种仪器都配备了DE20和DE12摄像机以集成模式运行。这些显微镜已在最先进的分辨率下有效地用于各种生物标本，包括大分子，分子机和细胞。我们的设施将分配给他提议的财团，同时考虑到接待机构正在进行的研究。我们的首席PI Wah Chiu是将冷冻和冷冻材料推向当前界限的公认专家，并且在领导几个NIH资助的研究中心和学术培训计划方面拥有数十年的经验。美国有11个参与机构，由在Cryoem和/或Cryoet中具有研究记录的调查人员领导。日常操作将由兼职冷冻/ET科学家和IT人员进行。亲自和远程数据收集协议都将用于遥远的用户。该财团将为遥远的用户提供旅行资金。我们要求用于以计数模式运行的直接检测设备（DDD）的资金，以满足所有类型的标本的需求。主机机构将提供一名行政助理，以支持用户访问和各种财团活动，并匹配资金以购买DDD和足够的硬盘驱动器以进行短期数据存储。参与的PI将参与制定财团治理和开发培训活动的特定政策。根据标本准备就绪，安排将在第一年的第一年平均分配，并具有一定的灵活性。显微镜时间和优先级的分配将在第二年及以后分配，以所有PIS任命的外部显微镜分配委员会的建议，以避免利益冲突。 Al PIS将在WebEX上季刊，讨论技术和行政问题，我们将举行年度用户会议。咨询委员会将对财团运营的各个方面以及用户的数据结果进行年度审查，以承担拟议设施的最高生产率和使用。参与机构的成员资格将由所有PI和外部咨询的投入进行动态审查 运营第二年后的委员会。