CryoEM Data Collection Facility Consortium at NCM

NCM 的 CryoEM 数据收集设施联盟

• 批准号: 9975875
• 负责人: ESTHER BULLITT
• 金额: $ 51.56万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9975875
• 关键词: Academic Training; Advisory Committees; Arbitration; Biological; Boston; Cells; Conflict (Psychology); Conflict of Interest; Critiques; Cryo-electron tomography; Cryoelectron Microscopy; Data; Data Collection; Data Storage and Retrieval; Department chair; Detection; Development; Devices; Distant; Educational workshop; Electron Microscope; Electrons; Environment; Funding; Image; Imaging technology; Infrastructure; Institution; Laboratories; Lead; Logistics; Mammalian Cell; Medical; Medical center; Medicine; Microscope; Mission; Missouri; Molecular Machines; Noise; Outcome; Participant; Persons; Phase; Policies; Productivity; Proteins; Protocols documentation; Readiness; Recommendation; Request for Applications; Research; Research Personnel; Resolution; Resource Allocation; Resources; Sampling; Schedule; Scientist; Services; Signal Transduction; Specialist; Specimen; Strategic Planning; Structure; Texas; Time; Training; Training Activity; Training Programs; Travel; United States National Institutes of Health; Universities; Virginia; Virus; Visit; base; cellular imaging; chaperonin; college; contrast imaging; cost; data acquisition; data exchange; data management; detector; electron tomography; experience; field emission gun; flexibility; image processing; improved; instrument; instrumentation; macromolecule; meetings; member; movie; operation; particle; public health relevance; structural biology; symposium; technology development

 DESCRIPTION (provided by applicant): The National Center for Macromolecular Imaging (NCMI) at Baylor College of Medicine is the host laboratory offering to provide a portion of the time on their JEM3200FSC and JEM2200FS electron microscopes for a cryo-electron microscopy (cryoEM) and tomography (cryoET) data acquisition facility Consortium. Both instruments are equipped with a field emission gun and an in-column energy filter, while the JEM2200FS also has a Zernike phase plate attachment. Both instruments are currently equipped with DE20 and DE12 cameras operated in integrating mode. These microscopes have been used productively for a variety of biological specimens, including macromolecules, molecular machines and cells, at state-of-the-art resolution. Our facilities will be allocated for he proposed Consortium while taking into account the ongoing research in the host institution. Our lead PI, Wah Chiu is a recognized expert in pushing cryoEM and cryoET beyond current boundaries and has decades of experience in leading several NIH funded research centers and academic training programs. There are 11 participating institutions across the USA, led by investigators who have a research track record in cryoEM and/or cryoET. Day-to-day operation will be carried out by a part-time cryoEM/ET scientist and IT staff. Both in person and remote data collection protocols will be used for distant users. The Consortium will provide travel funds for the distant users. We request funding for a Direct Detection Device (DDD) operated in counting mode to meet the needs of all types of specimens. The host Institution will provide an administrative assistant to support user visits and various Consortium activities, and matching funds to purchase the DDD and adequate hard drives for short-term data storage. The participating PIs will be involved in formulating the specific policies for Consortium governance and developing training activities. Scheduling will be allocated evenly in the first year among participants with some flexibility based on specimen readiness. Allocation of microscope times and priorities will be assigned in the second year and beyond based on recommendations of an external microscope allocation committee appointed by all the PIs to avoid conflict of interest. Al PIs will meet quarterly on WebEx to discuss technical and administrative issues, and we will hold an annual user meeting. An annual review of all aspects of the Consortium operation and the data outcomes of the users will be conducted by an advisory committee to assure the highest productivity and usage of the proposed facility. The membership of the participating institutions will be dynamically reviewed by all the PIs with the inputs from the external advisory committee after the second year of our operation.

 描述(由申请人提供)：位于贝勒医学院的国家大分子成像中心(NCMI)是东道主实验室，提供他们的JEM3200FSC和JEM2200FS电子显微镜的部分时间，用于冷冻电子显微镜(CryoEM)和断层扫描(CryoET)数据采集设施联合体。这两种仪器都配备了场发射枪和柱内能量过滤器，而JEM2200FS也配备了Zernike相位板附件。这两台仪器目前都配备了以集成模式运行的DE20和DE12摄像机。这些显微镜已经以最先进的分辨率有效地用于各种生物标本，包括大分子、分子机器和细胞。我们的设施将分配给拟议的财团，同时考虑到主办机构正在进行的研究。我们的首席PI Wah Chiu是一位在推动低温EM和低温ET突破现有界限方面的公认专家，他在领导多个NIH资助的研究中心和学术培训项目方面拥有数十年的经验。美国有11个参与机构，由研究人员领导，他们在低温EM和/或低温ET方面有研究记录。日常操作将由一名兼职的低温EM/ET科学家和IT人员进行。对于远程用户，将使用面对面和远程数据收集协议。该联盟将为远程用户提供旅行资金。我们要求提供资金，以计数模式运行的直接检测设备(DDD)，以满足所有类型的标本的需要。主办机构将提供一名行政助理，以支持用户访问和各种联合体活动，并为购买DDD和用于短期数据存储的足够硬盘提供配套资金。参与的私人投资机构将参与制定联合体治理的具体政策和开展培训活动。日程安排将在第一年在参与者之间平均分配，并根据样本准备情况有一定的灵活性。显微镜时间和优先次序的分配将在第二年及以后根据所有私人投资机构任命的外部显微镜分配委员会的建议进行分配，以避免利益冲突。AI将在WebEx上每季度开会一次，讨论技术和管理问题，我们将举行年度用户会议。咨询委员会将对联合体业务的所有方面和用户的数据结果进行年度审查，以确保拟议设施的最高生产率和使用率。参与机构的成员资格将由所有私人投资机构根据外部咨询的意见进行动态审查 在我们运作的第二年之后。

项目成果

Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis.

• DOI: 10.1093/nar/gkx785
• 发表时间: 2017-10-13
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Yang K;Chang JY;Cui Z;Li X;Meng R;Duan L;Thongchol J;Jakana J;Huwe CM;Sacchettini JC;Zhang J
• 通讯作者: Zhang J