The Southeastern Consortium for Microscopy of MacroMolecular Machines

摘要 东南大分子机器显微镜中心（SECM 4）是一个财团， 15所大学/医疗中心，共有19名研究者，遍布美国东部 国家研究了广泛的重要生物医学项目，如高分辨率 病毒结构，膜蛋白结构，各种类型的大分子复合物，一些 以活性形式从细胞、细菌超微结构、肌丝、剪接体 核糖体复合物，所有这些都将受益于随时获得高分辨率电子， 使用配备有直接电子检测器（DED）的Titan Krios等显微镜。人类健康 其影响从病毒和细菌病原体扩展到对疾病的理解 导致基因突变。癌症和心脏病的基础生物学正在研究中 在几个实验室。佛罗里达州立大学的泰坦克里奥斯已经在 自2009年开始运营，最近已将其图像记录设备从CCD升级为 直接电子有限责任公司，DE-20直接电子探测器定位在现有的 去除非弹性散射电子从而改善图像的成像滤波器 质量.虽然我们提出了一个强有力的计划，使成员来到佛罗里达州， 大学，我们建议建立一个设施的基础上，同步加速器模板目前在使用 全国各地的多个站点X射线晶体学光束线， 标本给我们，并观看数据收集，因为它来自显微镜， 熟悉自己实验室的范围。我们将提供充分的预处理， 联合体成员可以评估获得最终高分辨率结构的前景 从损坏和运动校正的“电影”图像的样品。结果将是一个模型 对于利用高端仪器的高通量结构测定， 复杂的大分子和亚细胞结构的内部运作。

Abstract The Southeastern Center for Microscopy of MacroMolecular Machines (SECM4) is a consortium of 15 Universities/Medical Centers with a total of 19 investigators throughout the Eastern United States studying a wide range of important biomedical projects as variable as high resolution virus structure, membrane protein structure, macromolecular complexes of various types, some isolated in active form from cells, bacterial ultrastructure, muscle filaments, spliceosomes, ribosomes complexes all of which will benefit from ready access to a high resolution electron microscope such as a Titan Krios equipped with a direct electron detector (DED). Human health implications extend from virus and bacterial pathogens to the understanding of diseases resulting form genetic mutations. The basic biology of cancer and heart disease is being studied in several member laboratories. The Titan Krios at Florida State University has been in operation since 2009 and recently has had its image recording device upgraded from CCD camera to a Direct Electron LLC, DE-20 direct electron detector positioned ahead of an existing imaging filter which removes inelastically scattered electrons thereby improving the image quality. Although we propose a robust plan to enable members to come to Florida State University, we propose creating a facility based on the synchrotron template currently in use at multiple sites X-ray crystallography beam lines around the country whereby users ship specimens to us and watch the data being collected as it comes off the microscope from the familiar confines of their own laboratories. We will provide sufficient preprocessing that consortium members can evaluate the prospects for obtaining a final high resolution structure from damage and motion corrected “movie” images of their samples. The result will be a model for high throughput structure determination utilizing high-end instrumentation that can reveal the inner workings of complex macromolecules and subcellular structures.