Morphological and Molecular Imaging System for in vivo Atherosclerosis Research
用于体内动脉粥样硬化研究的形态和分子成像系统
基本信息
- 批准号:9039653
- 负责人:
- 金额:$ 36.09万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-04-01 至 2019-03-31
- 项目状态:已结题
- 来源:
- 关键词:AlgorithmsApoptosisAreaArterial Fatty StreakArteriesAtherosclerosisBiochemicalBiological MarkersBlood VesselsCardiac Catheterization ProceduresCardiologyCardiovascular PathologyCathetersClinicalCollagenCoronaryDatabasesDevelopmentDyslipidemiasFamily suidaeFiberFluorescenceFluorescence SpectroscopyFutureHistopathologyImageImageryImaging technologyIn VitroInflammationInflammation ProcessLabelLeadLipidsLocal TherapyMacrophage ActivationModelingMolecularMolecular TargetMonitorMorbidity - disease rateMorphologic artifactsMorphologyMyocardial InfarctionNecrosisOptical Coherence TomographyOxidative StressPerformanceProcessProgressive DiseaseResearchResolutionRuptureSamplingSpeedStagingStatistical Data InterpretationStrokeSystemSystemic TherapyTestingThickThrombosisTimeUnited StatesWorkbasecalcificationdesignfluorescence imagingimaging modalityimaging systemimprovedin vivoin vivo imaginginstrumentlimb injurymolecular imagingmolecular markermortalitynoveloptical imagingoptical spectraprototypetreatment response
项目摘要
DESCRIPTION (provided by applicant): Atherosclerosis is the leading cause of morbidity and mortality in the United States and is characterized as a systemic, progressive disease process in which the arterial wall thickens through a process of inflammation, oxidative stress, and dyslipidemia. This process leads to plaque formation and flow limitation in the vessel lumen. The sudden rupture of this arterial plaques lead to thrombosis and sudden occlusion of the vessel and ultimately, in myocardial infarction, stroke, or limb injury. Future development of systemic or localized therapies for atherosclerosis will likely depend upon a more detailed understanding of plaque development. Improving the understanding of plaque development will require in-vivo monitoring of morphological, biochemical and functional/molecular changes accompanying plaque formation and/or response to treatments. Unfortunately, there is no current imaging modality (neither non-invasive nor intravascular) that can provide such level of plaque characterization. We propose to develop a novel imaging technology that will enable high-speed in vivo intravascular imaging of plaque morphology, biochemical composition and molecular activity, by combining optical coherence tomography (OCT) with endogenous and exogenous fluorescence lifetime imaging (FLIM). Intravascular OCT offers high-resolution intravascular imaging of atherosclerotic plaque. FLIM has been shown to be far less susceptible to artifacts endemic to in vivo imaging than steady-state fluorescence. FLIM imaging of endogenous plaque fluorescence allows quantifying plaque biochemical content. FLIM imaging of exogenous fluorescent tags labeling multiple molecular targets will allow monitoring plaque molecular activity. To develop and validate this novel intravascular imaging modality, the following three specific aims are proposed. Aim 1: To integrate OCT with endogenous FLIM imaging for non-destructive identification of the different types of atherosclerotic plaques. Aim 2: To design and build a high-speed optical imaging system, including a dual-mode fiber catheter, suitable for in vivo intravascular simultaneous and coregistered OCT and endogenous/exogenous FLIM imaging of atherosclerotic plaques. Aim 3: To quantify the capacity of the OCT/FLIM intravascular imaging system to monitor over time plaque morphology, biochemical composition and molecular activity in-vivo. We believe that the resulting intravascular imaging technology will enable comprehensive understanding of plaque development and may ultimately help facilitate the development of a cure for atherosclerosis.
描述(由申请方提供):动脉粥样硬化是美国发病率和死亡率的主要原因,其特征为一种全身性、进行性疾病过程,其中动脉壁通过炎症、氧化应激和血脂异常过程增厚。该过程导致斑块形成和血管腔中的流动限制。这种动脉斑块的突然破裂导致血栓形成和血管的突然闭塞,并最终导致心肌梗死、中风或肢体损伤。动脉粥样硬化的全身或局部治疗的未来发展可能取决于对斑块发展的更详细的了解。提高对斑块发展的理解将需要对伴随斑块形成和/或对治疗的反应的形态学、生物化学和功能/分子变化进行体内监测。不幸的是,目前还没有能够提供这种水平的斑块表征的成像模式(非侵入性的或血管内的)。我们建议开发一种新的成像技术,使高速在体内血管内成像的斑块形态,生化成分和分子活性,通过结合光学相干断层扫描(OCT)与内源性和外源性荧光寿命成像(FLIM)。血管内OCT提供动脉粥样硬化斑块的高分辨率血管内成像。FLIM已被证明比稳态荧光更不易受体内成像特有的伪影的影响。内源性斑块荧光的FLIM成像允许量化斑块生化含量。标记多个分子靶标的外源性荧光标签的FLIM成像将允许监测斑块分子活性。为了开发和验证这种新的血管内成像模式,提出了以下三个具体目标。目的1:将OCT与内源性FLIM成像相结合,用于非破坏性识别不同类型的动脉粥样硬化斑块。目标二:设计并构建一个高速光学成像系统,包括一个双模光纤导管,适用于动脉粥样硬化斑块的体内血管内同步和共配准OCT和内源性/外源性FLIM成像。目标三:量化OCT/FLIM血管内成像系统随时间监测斑块形态、生化成分和体内分子活性的能力。我们相信,由此产生的血管内成像技术将能够全面了解斑块的发展,并可能最终有助于促进治疗动脉粥样硬化的发展。
项目成果
期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Application of non-negative matrix factorization to multispectral FLIM data analysis.
- DOI:10.1364/boe.3.002244
- 发表时间:2012-09-01
- 期刊:
- 影响因子:3.4
- 作者:Pande P;Applegate BE;Jo JA
- 通讯作者:Jo JA
Multimodal optical coherence tomography and fluorescence lifetime imaging with interleaved excitation sources for simultaneous endogenous and exogenous fluorescence.
多模态光学相干断层扫描和荧光寿命成像,具有交错激发源,可同时进行内源性和外源性荧光。
- DOI:10.1364/boe.7.003184
- 发表时间:2016
- 期刊:
- 影响因子:3.4
- 作者:Shrestha,Sebina;Serafino,MichaelJ;Rico-Jimenez,Jesus;Park,Jesung;Chen,Xi;Zhaorigetu,Siqin;Walton,BrianL;Jo,JavierA;Applegate,BrianE
- 通讯作者:Applegate,BrianE
Quadratic blind linear unmixing: A graphical user interface for tissue characterization.
- DOI:10.1016/j.cmpb.2015.10.016
- 发表时间:2016-02
- 期刊:
- 影响因子:6.1
- 作者:Gutierrez-Navarro O;Campos-Delgado DU;Arce-Santana ER;Jo JA
- 通讯作者:Jo JA
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Brian E. Applegate其他文献
Analysis of ear symmetry as a diagnostic tool enabled by optical coherence tomography
通过光学相干断层扫描分析耳朵对称性作为诊断工具
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:0
- 作者:
Zihan Yang;Wihan Kim;Marcela A. Morán;Ryan Long;J. Oghalai;Brian E. Applegate - 通讯作者:
Brian E. Applegate
Visualizing motions within the cochlea's organ of Corti and illuminating cochlear mechanics with optical coherence tomography
- DOI:
10.1016/j.heares.2024.109154 - 发表时间:
2025-01-01 - 期刊:
- 影响因子:
- 作者:
Elizabeth S. Olson;Wei Dong;Brian E. Applegate;Karolina K. Charaziak;James B. Dewey;Brian L. Frost;Sebastiaan W.F. Meenderink;Jong-Hoon Nam;John S. Oghalai;Sunil Puria;Tianying Ren;C. Elliott Strimbu;Marcel van der Heijden - 通讯作者:
Marcel van der Heijden
Ex-vivo imaging of the human cochlea using 1.7μm SS-OCT
使用 1.7μm SS-OCT 对人类耳蜗进行离体成像
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:0
- 作者:
Jack C. Tang;Dorothy W. Pan;J. Oghalai;Brian E. Applegate - 通讯作者:
Brian E. Applegate
Morphological and biochemical imaging of coronary atherosclerotic plaques using optical coherence tomography and fluorescence lifetime imaging
- DOI:
10.1016/j.carrev.2009.04.053 - 发表时间:
2009-07-01 - 期刊:
- 影响因子:
- 作者:
Javier A. Jo;Brian E. Applegate;Fred Clubb - 通讯作者:
Fred Clubb
Brian E. Applegate的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Brian E. Applegate', 18)}}的其他基金
Ultra-stable, phase sensitive, snapshot OCT system enabled by 2-Photon additive manufacturing
通过 2 光子增材制造实现超稳定、相敏、快照 OCT 系统
- 批准号:
10607853 - 财政年份:2023
- 资助金额:
$ 36.09万 - 项目类别:
High-Speed, Low-Cost, Image Remapping Spectral Domain Full-Field Optical Coherence Tomography for Retinal Imaging
用于视网膜成像的高速、低成本图像重映射谱域全场光学相干断层扫描
- 批准号:
10670648 - 财政年份:2023
- 资助金额:
$ 36.09万 - 项目类别:
Comb Light Source/Imaging spectrometer for advanced Spectral Domain Optical Coherence Tomography
用于先进谱域光学相干断层扫描的梳状光源/成像光谱仪
- 批准号:
10058117 - 财政年份:2020
- 资助金额:
$ 36.09万 - 项目类别:
Comb Light Source/Imaging spectrometer for advanced Spectral Domain Optical Coherence Tomography
用于先进谱域光学相干断层扫描的梳状光源/成像光谱仪
- 批准号:
10242951 - 财政年份:2020
- 资助金额:
$ 36.09万 - 项目类别:
Optical imaging technologies to identify residual cholesteatoma and improve ossiculoplasty outcomes
光学成像技术可识别残余胆脂瘤并改善骨成形术结果
- 批准号:
9899243 - 财政年份:2019
- 资助金额:
$ 36.09万 - 项目类别:
Optical imaging technologies to identify residual cholesteatoma and improve ossiculoplasty outcomes
光学成像技术可识别残余胆脂瘤并改善骨成形术结果
- 批准号:
10373930 - 财政年份:2019
- 资助金额:
$ 36.09万 - 项目类别:
Morphological and Molecular Imaging System for in vivo Atherosclerosis Research
用于体内动脉粥样硬化研究的形态和分子成像系统
- 批准号:
8450783 - 财政年份:2012
- 资助金额:
$ 36.09万 - 项目类别:
Morphological and Molecular Imaging System for in vivo Atherosclerosis Research
用于体内动脉粥样硬化研究的形态和分子成像系统
- 批准号:
8645722 - 财政年份:2012
- 资助金额:
$ 36.09万 - 项目类别:
Morphological and Molecular Imaging System for in vivo Atherosclerosis Research
用于体内动脉粥样硬化研究的形态和分子成像系统
- 批准号:
8222478 - 财政年份:2012
- 资助金额:
$ 36.09万 - 项目类别:
Development of high-resolution molecular imaging of endogenous chromophores with
内源性发色团高分辨率分子成像的发展
- 批准号:
7845631 - 财政年份:2009
- 资助金额:
$ 36.09万 - 项目类别:
相似国自然基金
间充质干细胞凋亡小体来源的PRKAR2A蛋白调控Adrβ2+巨噬细胞M2极化促成骨的机制研究
- 批准号:
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
蛋白磷酸酶PP2Cm缺失加剧肝纤维化的作用机制研究
- 批准号:
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
半胱氨酸蛋白酶抑制剂B在缺血性脑卒中的功能及机制研究
- 批准号:JCZRLH202500240
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
“生地-天冬”配伍通过调控DPP4/CASP3抑制角质细胞凋亡从而防治皮肤光老化的作用机制
- 批准号:JCZRLH202500496
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
基于糖代谢重编程探究人参提取物RH2治疗黑色素瘤的机制
- 批准号:JCZRLH202500808
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
3D细胞球来源的凋亡小体修饰间充质干细胞的制备及“内外兼修”策略的构建用于脊髓损伤修复的作用机制研究
- 批准号:QN25H060008
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
鹰嘴豆芽素A靶向线粒体防控肺癌发生发展的机制研究
- 批准号:QN25H160055
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
间充质干细胞源性凋亡小体调控成纤维细胞NAD代谢治疗瘢痕疙瘩的机制研究
- 批准号:MS25H150038
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
CD53+中性粒细胞调节奶牛乳腺上皮凋亡的作用机制研究
- 批准号:R25C170001
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
吲哚-3-乙酸通过miR-9-5p-SIRT1-FOXO1通路调控糖尿病心肌病自噬凋亡失衡的机制研究
- 批准号:
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
相似海外基金
Milk fat globule-EGF factor 8 and hepatocyte apoptosis-induced liver wound healing response
乳脂肪球-EGF因子8与肝细胞凋亡诱导的肝脏创面愈合反应
- 批准号:
10585802 - 财政年份:2023
- 资助金额:
$ 36.09万 - 项目类别:
Development of an apoptosis biosensor for monitoring of breast cancer
开发用于监测乳腺癌的细胞凋亡生物传感器
- 批准号:
10719415 - 财政年份:2023
- 资助金额:
$ 36.09万 - 项目类别:
Interrogating the Fgl2-FcγRIIB axis on CD8+ T cells: A novel mechanism mediating apoptosis of tumor-specific memory CD8+ T cells
询问 CD8 T 细胞上的 Fgl2-FcγRIIB 轴:介导肿瘤特异性记忆 CD8 T 细胞凋亡的新机制
- 批准号:
10605856 - 财政年份:2023
- 资助金额:
$ 36.09万 - 项目类别:
Novel targeted therapy for FGFR inhibitor-resistant urothelial cancer and apoptosis based therapy for urothelial cancer
FGFR抑制剂耐药性尿路上皮癌的新型靶向治疗和基于细胞凋亡的尿路上皮癌治疗
- 批准号:
23K08773 - 财政年份:2023
- 资助金额:
$ 36.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanistic analysis of apoptosis induction by HDAC inhibitors in head and neck cancer
HDAC抑制剂诱导头颈癌凋亡的机制分析
- 批准号:
23K15866 - 财政年份:2023
- 资助金额:
$ 36.09万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Interrogating the Fgl2-FcgRIIB axis: A novel mechanism mediating apoptosis of tumor-specific memory CD8+ T cells
探究 Fgl2-FcgRIIB 轴:介导肿瘤特异性记忆 CD8 T 细胞凋亡的新机制
- 批准号:
10743485 - 财政年份:2023
- 资助金额:
$ 36.09万 - 项目类别:
Investigating the role of apoptosis-resistance and the tumor environment on development and maintenance of sacrococcygeal teratomas
研究细胞凋亡抗性和肿瘤环境对骶尾部畸胎瘤发生和维持的作用
- 批准号:
10749797 - 财政年份:2023
- 资助金额:
$ 36.09万 - 项目类别:
The effects of glucose on immune cell apoptosis and mitochondrial membrane potential and the analysis of its mechanism by which glucose might modulate the immune functions.
葡萄糖对免疫细胞凋亡和线粒体膜电位的影响及其调节免疫功能的机制分析。
- 批准号:
22K09076 - 财政年份:2022
- 资助金额:
$ 36.09万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
XAF1 IN P53 SIGNALING, APOPTOSIS AND TUMOR SUPPRESSION
P53 信号传导、细胞凋亡和肿瘤抑制中的 XAF1
- 批准号:
10583516 - 财政年份:2022
- 资助金额:
$ 36.09万 - 项目类别:
Role of Thioredoxin system in regulation of autophagy-apoptosis cross talk in neurons: Uncovering Novel Molecular Interactions.
硫氧还蛋白系统在神经元自噬-凋亡串扰调节中的作用:揭示新的分子相互作用。
- 批准号:
RGPIN-2019-05371 - 财政年份:2022
- 资助金额:
$ 36.09万 - 项目类别:
Discovery Grants Program - Individual