CORE B - Stem Cell and Trophoblast Analysis Core

项目摘要/摘要(核心B) 干细胞和滋养层细胞分析核心单元将促进小鼠和人类所有方面的研究 胚胎干细胞(ES)和啮齿动物滋养层干细胞(TS)。干细胞与滋养层细胞分析 核心单位将加强研究项目的实验能力。每一项研究项目 建议在他们的研究中使用干细胞。核心将通过以下方式为研究工作做出贡献 下列活动：一)细胞培养液和相关试剂的获取和制备；二)维护 干细胞储存和细胞培养方案的标准化；三)细胞培养的支原体检测；四)细胞 用于大规模实验的操作和收获；v)监督和计划共享的使用 设备；六)协调干细胞的全基因组测序和生物信息学分析；七) 研究滋养细胞谱系发育的新策略的发展。获得干细胞和 滋养层细胞分析核心单元将是执行基本方面的最具成本效益的方法 拟议的实验。标准化试剂、程序、样品采集和操作 必要的仪器设备将显著提高实验的重复性。这将生成一个 极大地节省了试剂成本和人力时间。这样的做法也将大大改善 实验设计、故障排除实验和结果解释。

PROJECT SUMMARY/ABSTRACT (CORE B) The Stem Cell and Trophoblast Analysis Core Unit will facilitate all aspects of research with mouse and human embryonic stem (ES) cells and rodent trophoblast stem (TS) cells. The Stem Cell and Trophoblast Analysis Core Unit will enhance the experimental capabilities of the research projects. Each of the research projects proposes to use stem cells in their research. The Core will contribute to the research effort through the following activities: i) acquisition and preparation of cell culture medium and related reagents; ii) maintaining stem cell stocks and standardization of cell culture protocols; iii) mycoplasma testing of cell cultures; iv) cell manipulation and harvesting for large-scale experimentation; v) oversee and schedule use of shared equipment; vi) coordination of genome-wide sequencing and bioinformatic analyses of stem cells; vii) development of new strategies for studying trophoblast lineage development. Access to the Stem Cell and Trophoblast Analysis Core Unit will be the most cost effective method to carry out essential aspects of the proposed experimentation. Standardizing reagents, procedures, collection of samples, and operation of essential instrumentation will significantly improve the reproducibility of the experiments. This will generate a tremendous savings in reagent costs and manpower hours. Such practices will also greatly improve experimental design, troubleshooting experiments, and interpretation of results.