CORE B - Stem Cell and Trophoblast Analysis Core

CORE B - 干细胞和滋养层分析核心

• 批准号: 8897427
• 负责人: MICHAEL W WOLFE
• 金额: $ 18.17万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8897427
• 关键词: Bioinformatics; Cell Culture Techniques; Cells; Culture Media; Development; Epigenetic Process; Equipment; Experimental Designs; Harvest; Hour; Human Resources; Methods; Mus; Mycoplasma; Preparation; Procedures; Productivity; Protocols documentation; Reagent; Reproducibility; Research; Research Personnel; Research Project Grants; Rodent; Savings; Schedule; Services; Standardization; Stem cells; Testing; abstracting; cost; cost effective; genome-wide; human embryonic stem cell; improved; instrumentation; operation; repository; research study; sample collection; stem cell population; trophoblast

PROJECT SUMMARY/ABSTRACT (CORE B) The Stem Cell and Trophoblast Analysis Core Unit will facilitate all aspects of research with mouse and human embryonic stem (ES) cells and rodent trophoblast stem (TS) cells. The Stem Cell and Trophoblast Analysis Core Unit will enhance the experimental capabilities of the research projects. Each of the research projects proposes to use stem cells in their research. The Core will contribute to the research effort through the following activities: i) acquisition and preparation of cell culture medium and related reagents; ii) maintaining stem cell stocks and standardization of cell culture protocols; iii) mycoplasma testing of cell cultures; iv) cell manipulation and harvesting for large-scale experimentation; v) oversee and schedule use of shared equipment; vi) coordination of genome-wide sequencing and bioinformatic analyses of stem cells; vii) development of new strategies for studying trophoblast lineage development. Access to the Stem Cell and Trophoblast Analysis Core Unit will be the most cost effective method to carry out essential aspects of the proposed experimentation. Standardizing reagents, procedures, collection of samples, and operation of essential instrumentation will significantly improve the reproducibility of the experiments. This will generate a tremendous savings in reagent costs and manpower hours. Such practices will also greatly improve experimental design, troubleshooting experiments, and interpretation of results.

项目摘要/摘要（核心b）